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The map label for this gene is lcfB [H]

Identifier: 124486051

GI number: 124486051

Start: 1259806

End: 1261494

Strand: Reverse

Name: lcfB [H]

Synonym: Mlab_1231

Alternate gene names: 124486051

Gene position: 1261494-1259806 (Counterclockwise)

Preceding gene: 124486052

Following gene: 124486050

Centisome position: 69.89

GC content: 54.41

Gene sequence:

>1689_bases
ATGTCACGTCCTGTGTTTAGAGACGACGCGAAGACCGAACTGGAATACACCGACGAGACGAAACAGTCTCTCTATTCCAT
GTTCAACTACGGCGTGCAGCATGCAGGCCGCGAGGCCGTGGCCATTCAATACTTCGACAACCGGATCACGTACGGGGAAC
TGTATGATCTCATCGACACCTGTGCGGCGGGTTTTCTCGATCGCGGCGTAAAGAAAGGCGATTTCGTCACGCTCTTTCTG
CCGAACATCCCGCAGTGCGTGATTGCGGTCTATGCCCTGAACCGGATCGGTGCAATATGCAATATGGTCCACCCGCTCTC
GACCCTGTCGGAGCTGGAGAATGCGGTCAAACTCACGGACAGCAAACTCATCCTCACGTTTGAACTGAACGAAGGTCTTG
CGGCCAACTTCGATGTGGATATCATCCGGTGCAGAACGCCGGGCTACTTCCCGCCGGGTCCAAAGGGTTTCGTGATGAAA
TCCGTGTATTCCTACACGGTCCGAAAATCGCCGAAGGCTTCGTCCCGCGTCACCGAGTGGGCCGATCTTCTGGCGGCCGG
GAAAAAGCGGTTCCAGACAACGCCTCTCCCGCCTTTAGAAGGAAAAGCAGACGATACGGCCGTCATTATGTACACCGGCG
GCACGACCGGACCCGCGAAAGGGGTCATGATCTCGAACTGGTCGGTGAATTACGTCACGACGCGTCTTCTTCTGGAGAAC
GTCACGCACACCGCCCATATCGGCGACGGATTCCTCGCGATCCTTCCGCTCTTCCATGCGTTTGGGCTTGCGGTCTGTAT
CCAGGCCCCGCTTTCCTCCGGGATGCGGGTCATGCTTTCCCCCCGGTTCAATGATAAAGAGTGCAGCAGTCTTCTTCTGA
AAGAGAAGGTCGCCTATGTGATCGGCGTTCCCGCGATGTACGAACGGATGTACCCGTATCTGAAAGATCACGACCTTTCA
TTCATGAAGCACGTTGTCTGCGGAGGAGACTGGGTAAGCCATGACCTCGCCTACCGGTATAATGACATCTTAGGAAAGGA
CAAGGGAGGTGCGGAGTTCCGTCCGGGTTACGGTCTGACCGAGGCGTGCGGAACCTGCTCTCTTACGCGGAATCAGTATA
AGGCATTTCCCGAAGGATGCGTGGGTATCCCTGTGGAAGGAACTGACATCTGCCTTGTGAACCCCGGAACCTTTGATGAG
GTTCCCGAGGGTGAAGAAGGGGAGCTTTGCATCTCCAGTCCCTCGGTGATGAAAGGATATTATAAAAATCCCGAGGCAAC
AGACGATGTCTTAAAGATTCATCCCGACGGAAAACTCTGGCTCCATACGGGAGACATATTCGCGATCGGCGAGGAGAACA
ATCTCTGCTTTAGATCCCGCATCAAGCGTCTGGTCAAGGTGAACGGATATAACGTCTATCCGCCCTTAATCGAAGCGGCA
ATGGAAGGCTGTCCGATCGTTGCCAAAGCCTGCGCCGTAGGCTTCAAGTGGCGGGACGACCGGCGGATCAAACTGTATGT
GACCCTGAAGCAGAAGATGGATCATGCCGAAGCGGAGAAACATCTGCTTGCCTTTGCGACCGAACATCTGAATCACTGGA
GTGTTCCAAAAGCGTTTGTCATCCTTGATGAGATGCCGATGACGAAGATGAACAAGCTCGATTATATGGCGCTCCAGGAT
AGGGCCTAA

Upstream 100 bases:

>100_bases
GGCCGATCTTCCCATGACCAAGTTCAACAAGATAGATTACCGTCTGCTGGAAAAGCAGGAACTTTCACGGGCAAACGATA
AAAAGCAGGCTGAGTAACGT

Downstream 100 bases:

>100_bases
CCTTTTTTTTCATACCGTCCCCCAACGAGGGGCCAATTTCGGGCTGAAGTATAAATACTCTCTGGTCATTGCCTCTGACA
TTATTTTTTGCAAAATTTGT

Product: precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSRPVFRDDAKTELEYTDETKQSLYSMFNYGVQHAGREAVAIQYFDNRITYGELYDLIDTCAAGFLDRGVKKGDFVTLFL
PNIPQCVIAVYALNRIGAICNMVHPLSTLSELENAVKLTDSKLILTFELNEGLAANFDVDIIRCRTPGYFPPGPKGFVMK
SVYSYTVRKSPKASSRVTEWADLLAAGKKRFQTTPLPPLEGKADDTAVIMYTGGTTGPAKGVMISNWSVNYVTTRLLLEN
VTHTAHIGDGFLAILPLFHAFGLAVCIQAPLSSGMRVMLSPRFNDKECSSLLLKEKVAYVIGVPAMYERMYPYLKDHDLS
FMKHVVCGGDWVSHDLAYRYNDILGKDKGGAEFRPGYGLTEACGTCSLTRNQYKAFPEGCVGIPVEGTDICLVNPGTFDE
VPEGEEGELCISSPSVMKGYYKNPEATDDVLKIHPDGKLWLHTGDIFAIGEENNLCFRSRIKRLVKVNGYNVYPPLIEAA
MEGCPIVAKACAVGFKWRDDRRIKLYVTLKQKMDHAEAEKHLLAFATEHLNHWSVPKAFVILDEMPMTKMNKLDYMALQD
RA

Sequences:

>Translated_562_residues
MSRPVFRDDAKTELEYTDETKQSLYSMFNYGVQHAGREAVAIQYFDNRITYGELYDLIDTCAAGFLDRGVKKGDFVTLFL
PNIPQCVIAVYALNRIGAICNMVHPLSTLSELENAVKLTDSKLILTFELNEGLAANFDVDIIRCRTPGYFPPGPKGFVMK
SVYSYTVRKSPKASSRVTEWADLLAAGKKRFQTTPLPPLEGKADDTAVIMYTGGTTGPAKGVMISNWSVNYVTTRLLLEN
VTHTAHIGDGFLAILPLFHAFGLAVCIQAPLSSGMRVMLSPRFNDKECSSLLLKEKVAYVIGVPAMYERMYPYLKDHDLS
FMKHVVCGGDWVSHDLAYRYNDILGKDKGGAEFRPGYGLTEACGTCSLTRNQYKAFPEGCVGIPVEGTDICLVNPGTFDE
VPEGEEGELCISSPSVMKGYYKNPEATDDVLKIHPDGKLWLHTGDIFAIGEENNLCFRSRIKRLVKVNGYNVYPPLIEAA
MEGCPIVAKACAVGFKWRDDRRIKLYVTLKQKMDHAEAEKHLLAFATEHLNHWSVPKAFVILDEMPMTKMNKLDYMALQD
RA
>Mature_561_residues
SRPVFRDDAKTELEYTDETKQSLYSMFNYGVQHAGREAVAIQYFDNRITYGELYDLIDTCAAGFLDRGVKKGDFVTLFLP
NIPQCVIAVYALNRIGAICNMVHPLSTLSELENAVKLTDSKLILTFELNEGLAANFDVDIIRCRTPGYFPPGPKGFVMKS
VYSYTVRKSPKASSRVTEWADLLAAGKKRFQTTPLPPLEGKADDTAVIMYTGGTTGPAKGVMISNWSVNYVTTRLLLENV
THTAHIGDGFLAILPLFHAFGLAVCIQAPLSSGMRVMLSPRFNDKECSSLLLKEKVAYVIGVPAMYERMYPYLKDHDLSF
MKHVVCGGDWVSHDLAYRYNDILGKDKGGAEFRPGYGLTEACGTCSLTRNQYKAFPEGCVGIPVEGTDICLVNPGTFDEV
PEGEEGELCISSPSVMKGYYKNPEATDDVLKIHPDGKLWLHTGDIFAIGEENNLCFRSRIKRLVKVNGYNVYPPLIEAAM
EGCPIVAKACAVGFKWRDDRRIKLYVTLKQKMDHAEAEKHLLAFATEHLNHWSVPKAFVILDEMPMTKMNKLDYMALQDR
A

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=25.5474452554745, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI187761345, Length=400, Percent_Identity=24.75, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI187761343, Length=400, Percent_Identity=24.75, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4758332, Length=373, Percent_Identity=25.7372654155496, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI12669909, Length=373, Percent_Identity=25.7372654155496, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788107, Length=576, Percent_Identity=29.8611111111111, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789201, Length=557, Percent_Identity=24.9551166965889, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693145, Length=557, Percent_Identity=22.8007181328546, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=25.6214149139579, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790505, Length=579, Percent_Identity=22.9706390328152, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=397, Percent_Identity=25.9445843828715, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=26.3157894736842, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=527, Percent_Identity=23.3396584440228, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=544, Percent_Identity=24.8161764705882, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=528, Percent_Identity=23.4848484848485, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=556, Percent_Identity=23.2014388489209, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=468, Percent_Identity=24.1452991452991, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=21.9924812030075, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=21.9924812030075, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=21.9924812030075, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=523, Percent_Identity=23.9005736137667, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17553312, Length=373, Percent_Identity=26.5415549597855, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=306, Percent_Identity=24.8366013071895, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=539, Percent_Identity=25.417439703154, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=557, Percent_Identity=23.6983842010772, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=22.8650137741047, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI18859661, Length=550, Percent_Identity=23.8181818181818, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581924, Length=581, Percent_Identity=23.9242685025818, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355181, Length=490, Percent_Identity=25.3061224489796, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356441, Length=396, Percent_Identity=24.4949494949495, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922652, Length=376, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281365686, Length=342, Percent_Identity=26.6081871345029, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648676, Length=379, Percent_Identity=24.0105540897098, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24656500, Length=386, Percent_Identity=25.3886010362694, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356947, Length=315, Percent_Identity=25.3968253968254, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21358303, Length=446, Percent_Identity=24.6636771300448, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648260, Length=415, Percent_Identity=24.578313253012, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653035, Length=299, Percent_Identity=25.0836120401338, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648253, Length=457, Percent_Identity=23.1947483588621, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355225, Length=183, Percent_Identity=30.0546448087432, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648255, Length=457, Percent_Identity=23.4135667396061, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161076582, Length=322, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 62658; Mature: 62527

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPVFRDDAKTELEYTDETKQSLYSMFNYGVQHAGREAVAIQYFDNRITYGELYDLIDT
CCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
CAAGFLDRGVKKGDFVTLFLPNIPQCVIAVYALNRIGAICNMVHPLSTLSELENAVKLTD
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
SKLILTFELNEGLAANFDVDIIRCRTPGYFPPGPKGFVMKSVYSYTVRKSPKASSRVTEW
CEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADLLAAGKKRFQTTPLPPLEGKADDTAVIMYTGGTTGPAKGVMISNWSVNYVTTRLLLEN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHH
VTHTAHIGDGFLAILPLFHAFGLAVCIQAPLSSGMRVMLSPRFNDKECSSLLLKEKVAYV
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
IGVPAMYERMYPYLKDHDLSFMKHVVCGGDWVSHDLAYRYNDILGKDKGGAEFRPGYGLT
CCCHHHHHHHCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EACGTCSLTRNQYKAFPEGCVGIPVEGTDICLVNPGTFDEVPEGEEGELCISSPSVMKGY
HHHCCCCCCHHHHHCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHCC
YKNPEATDDVLKIHPDGKLWLHTGDIFAIGEENNLCFRSRIKRLVKVNGYNVYPPLIEAA
CCCCCCCCCEEEECCCCEEEEEECCEEEEECCCCCHHHHHHHHHHEECCCEECHHHHHHH
MEGCPIVAKACAVGFKWRDDRRIKLYVTLKQKMDHAEAEKHLLAFATEHLNHWSVPKAFV
HCCCCHHHHHHHCCCEECCCCEEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILDEMPMTKMNKLDYMALQDRA
EEECCCCHHHCCCCEEEECCCC
>Mature Secondary Structure 
SRPVFRDDAKTELEYTDETKQSLYSMFNYGVQHAGREAVAIQYFDNRITYGELYDLIDT
CCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
CAAGFLDRGVKKGDFVTLFLPNIPQCVIAVYALNRIGAICNMVHPLSTLSELENAVKLTD
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
SKLILTFELNEGLAANFDVDIIRCRTPGYFPPGPKGFVMKSVYSYTVRKSPKASSRVTEW
CEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADLLAAGKKRFQTTPLPPLEGKADDTAVIMYTGGTTGPAKGVMISNWSVNYVTTRLLLEN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHH
VTHTAHIGDGFLAILPLFHAFGLAVCIQAPLSSGMRVMLSPRFNDKECSSLLLKEKVAYV
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
IGVPAMYERMYPYLKDHDLSFMKHVVCGGDWVSHDLAYRYNDILGKDKGGAEFRPGYGLT
CCCHHHHHHHCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
EACGTCSLTRNQYKAFPEGCVGIPVEGTDICLVNPGTFDEVPEGEEGELCISSPSVMKGY
HHHCCCCCCHHHHHCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHCC
YKNPEATDDVLKIHPDGKLWLHTGDIFAIGEENNLCFRSRIKRLVKVNGYNVYPPLIEAA
CCCCCCCCCEEEECCCCEEEEEECCEEEEECCCCCHHHHHHHHHHEECCCEECHHHHHHH
MEGCPIVAKACAVGFKWRDDRRIKLYVTLKQKMDHAEAEKHLLAFATEHLNHWSVPKAFV
HCCCCHHHHHHHCCCEECCCCEEEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILDEMPMTKMNKLDYMALQDRA
EEECCCCHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]