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The map label for this gene is cobB [H]

Identifier: 124485892

GI number: 124485892

Start: 1089011

End: 1090312

Strand: Reverse

Name: cobB [H]

Synonym: Mlab_1072

Alternate gene names: 124485892

Gene position: 1090312-1089011 (Counterclockwise)

Preceding gene: 124485904

Following gene: 124485891

Centisome position: 60.41

GC content: 52.76

Gene sequence:

>1302_bases
ATGATTAGACAAAGCACCAGCATCCCCCGTATCGTGATCGCCGGCACTCACAGCGGATGCGGAAAAACAACCACTGCGTC
CGGGATCATGGCGGCACTTCGGGCGAGGGGTCTCGTGGTCCAGCCGTTCAAAGTCGGACCGGATTTTATCGACCCGTCCC
ATCATTCGGTCGTTTGCGGGAGAAGTTCGCGCAATCTCGATCCGTTCATGATGGGTGAAGAGTCGGTCCGCCGTTCGTTT
GTTTCTGCATGCGAGGGGGCAGACATTGCTGTAATCGAGGGTGTGATGGGGTTGTATGACGGCGTGGATGGGGGGGACTG
CGCGAGCACGGCGCATGTCGCACGCATTCTCGATGCTCCTGTTGTTTTGGTTACAGACATCAAAGGCATGAGCCGGAGTG
TGGCCGCTTTGATCAAGGGGTACACGGAATTTGATTCCAGAGTCCGGTTTGCCGGCGTAATTATGACGAAAGGGGGCAGT
GAGAAGCATAAAGCGATGACGACCACGGATATTTCCGTCCCTCTTCTCGGATGGATCCCGCGAAGCGATGCACTCGCCGT
TGAAAGCCGGCACTTGGGTTTGAAGATGGCGGATGAGGATGCTCGGATGAAAGATACCGGAGCATTCATCGAAGAACACT
GCCTGCTTGACGCCCTTTTGGCCGCTGCTGCCGATACAGCAGTGATCACGGCAGAGACGAACATGCCCCCTGCAGAAAAC
AAAATCAGAATAGGTATCGCGCTGGACGAGGCGTTCTGTTTTTATTATCAGGACAACTTCGATCATCTCAGAAAGAAAGG
AGCCGAACTGATTTTCTTTTCTCCCATTCACGACACTCTTCCTGAAGCAGATGCCTATTACTTCGGCGGGGGTTATCCGG
AACTCTATGCCGAAGAACTGGCGAAATCCCGGTGCAGGTCTGAGCTGAAATGGGCTGCCGATTCAGGTAAATCGATTTTT
GGCGAGTGCGGCGGTCTGATGTGGCTGAATCGTTCGATAACTACACTGGAAGGGCGTAATTATAAAATGACTGGGCTTCT
TGATGCTGACTGTCAGATGGAAAAACGGTTCGTCGCTTTGAATTATGTTGTTGGTAAAGTGACGGCCCCCTCATTTTTCC
CTGAGGGGATGGATATTCGCGGTCATGAGTTCCACTACTCGCGAACCATACCCGATTCAGACGTACGATATGCGCTCACC
CTGACCCGCGGTGAAGGGGTCGGTAATGGAAAAGACGGGATTATGGCTGGTTCAGTGATCGGTGCATATACACACATGTA
CTTCGGCGCAGTCAATAAGTAA

Upstream 100 bases:

>100_bases
TTGGCAGAAACCCTTCCATAATATGCATAAAACACCTAGTATCTATCGATAAGATATAATTCTATAAAATTAAACTCAAA
CTGATATAAGGTAAACGATT

Downstream 100 bases:

>100_bases
AATTGATTCTTGGCGAAGAACGAAAATAGTATAGATCTTAATATCTGGTGTTATTTTGCATCCCGGAGAAGAACGAATAT
CATTTAAAAAAACCCTGTTG

Product: cobyrinate a,c-diamide synthase / hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIRQSTSIPRIVIAGTHSGCGKTTTASGIMAALRARGLVVQPFKVGPDFIDPSHHSVVCGRSSRNLDPFMMGEESVRRSF
VSACEGADIAVIEGVMGLYDGVDGGDCASTAHVARILDAPVVLVTDIKGMSRSVAALIKGYTEFDSRVRFAGVIMTKGGS
EKHKAMTTTDISVPLLGWIPRSDALAVESRHLGLKMADEDARMKDTGAFIEEHCLLDALLAAAADTAVITAETNMPPAEN
KIRIGIALDEAFCFYYQDNFDHLRKKGAELIFFSPIHDTLPEADAYYFGGGYPELYAEELAKSRCRSELKWAADSGKSIF
GECGGLMWLNRSITTLEGRNYKMTGLLDADCQMEKRFVALNYVVGKVTAPSFFPEGMDIRGHEFHYSRTIPDSDVRYALT
LTRGEGVGNGKDGIMAGSVIGAYTHMYFGAVNK

Sequences:

>Translated_433_residues
MIRQSTSIPRIVIAGTHSGCGKTTTASGIMAALRARGLVVQPFKVGPDFIDPSHHSVVCGRSSRNLDPFMMGEESVRRSF
VSACEGADIAVIEGVMGLYDGVDGGDCASTAHVARILDAPVVLVTDIKGMSRSVAALIKGYTEFDSRVRFAGVIMTKGGS
EKHKAMTTTDISVPLLGWIPRSDALAVESRHLGLKMADEDARMKDTGAFIEEHCLLDALLAAAADTAVITAETNMPPAEN
KIRIGIALDEAFCFYYQDNFDHLRKKGAELIFFSPIHDTLPEADAYYFGGGYPELYAEELAKSRCRSELKWAADSGKSIF
GECGGLMWLNRSITTLEGRNYKMTGLLDADCQMEKRFVALNYVVGKVTAPSFFPEGMDIRGHEFHYSRTIPDSDVRYALT
LTRGEGVGNGKDGIMAGSVIGAYTHMYFGAVNK
>Mature_433_residues
MIRQSTSIPRIVIAGTHSGCGKTTTASGIMAALRARGLVVQPFKVGPDFIDPSHHSVVCGRSSRNLDPFMMGEESVRRSF
VSACEGADIAVIEGVMGLYDGVDGGDCASTAHVARILDAPVVLVTDIKGMSRSVAALIKGYTEFDSRVRFAGVIMTKGGS
EKHKAMTTTDISVPLLGWIPRSDALAVESRHLGLKMADEDARMKDTGAFIEEHCLLDALLAAAADTAVITAETNMPPAEN
KIRIGIALDEAFCFYYQDNFDHLRKKGAELIFFSPIHDTLPEADAYYFGGGYPELYAEELAKSRCRSELKWAADSGKSIF
GECGGLMWLNRSITTLEGRNYKMTGLLDADCQMEKRFVALNYVVGKVTAPSFFPEGMDIRGHEFHYSRTIPDSDVRYALT
LTRGEGVGNGKDGIMAGSVIGAYTHMYFGAVNK

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: NA

Molecular weight: Translated: 46945; Mature: 46945

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQSTSIPRIVIAGTHSGCGKTTTASGIMAALRARGLVVQPFKVGPDFIDPSHHSVVCG
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEC
RSSRNLDPFMMGEESVRRSFVSACEGADIAVIEGVMGLYDGVDGGDCASTAHVARILDAP
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
VVLVTDIKGMSRSVAALIKGYTEFDSRVRFAGVIMTKGGSEKHKAMTTTDISVPLLGWIP
EEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHCEEEEECCCEEEEEECC
RSDALAVESRHLGLKMADEDARMKDTGAFIEEHCLLDALLAAAADTAVITAETNMPPAEN
CCCCEEEEHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KIRIGIALDEAFCFYYQDNFDHLRKKGAELIFFSPIHDTLPEADAYYFGGGYPELYAEEL
EEEEEEEEECEEEEEEECCHHHHHHCCCCEEEECCHHHHCCCCCEEEECCCCHHHHHHHH
AKSRCRSELKWAADSGKSIFGECGGLMWLNRSITTLEGRNYKMTGLLDADCQMEKRFVAL
HHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCEEEECCCCEEEEEEECCCCHHHHHEEEE
NYVVGKVTAPSFFPEGMDIRGHEFHYSRTIPDSDVRYALTLTRGEGVGNGKDGIMAGSVI
EEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEECHHH
GAYTHMYFGAVNK
HHHHHHHHCCCCC
>Mature Secondary Structure
MIRQSTSIPRIVIAGTHSGCGKTTTASGIMAALRARGLVVQPFKVGPDFIDPSHHSVVCG
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEC
RSSRNLDPFMMGEESVRRSFVSACEGADIAVIEGVMGLYDGVDGGDCASTAHVARILDAP
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
VVLVTDIKGMSRSVAALIKGYTEFDSRVRFAGVIMTKGGSEKHKAMTTTDISVPLLGWIP
EEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHCEEEEECCCEEEEEECC
RSDALAVESRHLGLKMADEDARMKDTGAFIEEHCLLDALLAAAADTAVITAETNMPPAEN
CCCCEEEEHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KIRIGIALDEAFCFYYQDNFDHLRKKGAELIFFSPIHDTLPEADAYYFGGGYPELYAEEL
EEEEEEEEECEEEEEEECCHHHHHHCCCCEEEECCHHHHCCCCCEEEECCCCHHHHHHHH
AKSRCRSELKWAADSGKSIFGECGGLMWLNRSITTLEGRNYKMTGLLDADCQMEKRFVAL
HHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCEEEECCCCEEEEEEECCCCHHHHHEEEE
NYVVGKVTAPSFFPEGMDIRGHEFHYSRTIPDSDVRYALTLTRGEGVGNGKDGIMAGSVI
EEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEECHHH
GAYTHMYFGAVNK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]