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The map label for this gene is grpE [H]

Identifier: 124485765

GI number: 124485765

Start: 942998

End: 943603

Strand: Reverse

Name: grpE [H]

Synonym: Mlab_0944

Alternate gene names: 124485765

Gene position: 943603-942998 (Counterclockwise)

Preceding gene: 124485766

Following gene: 124485764

Centisome position: 52.28

GC content: 47.85

Gene sequence:

>606_bases
ATGGGTGTGCAAACAAAACAAATATATAGAACTGCAATACAACAAATATGTGCAACAAGCGAAACAGGAAAAGCCATGGA
CAAACACACCGAGCCTGCGGAGGTCAAAAACGTTGACGCTTCGAAGGCAGAAACCCAAAACCCGGATGAGAATCCCGTTC
CTGAAACCACTGTTGTTGACGAACTCACAAAAAAATACGATGAACTCAATGACAAACATCTTCGTCTCGCAGCCGAATTC
GAAAATTACAAGAAGCGTGCCAAACGCGATCAGGAAAGTGCTGTCAGATATGCAAACGAGAAGTTTGCGCTTGACATCAT
CGACGTCCTCGACAACTTCGAACGTGCTCTGAAAAGCGATGACGAAAATCTTCGTGACGGATTAGAGCAGATCCACAAAC
TGTATCTCTCCATCCTTTCGAGAAACGGCATCGAACCAATGAAAATCACGGGAACAACATTCGACCCCGCATTCCATGAA
GCGGTCGCCTGCATCCCCGCAGACGCTCCCGAGGGTGCGATCATCGACGTCGCCGTTCCCGGATACATGATCCGCGACAA
GGTGCTTCGTCACGCAAAAGTGGCCGTAGCGAAGAAAAAAGAATAA

Upstream 100 bases:

>100_bases
AAACGGATAATTTCATTTCCGGTTCATTTTTTCATCTCAACGCGCCTTTTTCTCTTGAATCTTCGGGATTCTCTCTGATA
GTGGGTACTTCTGCTGATAT

Downstream 100 bases:

>100_bases
TAAAAGGTACTAAAAATGGCATCAAACAAAGTAATCGGAATTGATCTTGGAACCACCAACTCCTGCCTTGCGATTATGGA
AGGCGGAAGCCCGATCGTCA

Product: hypothetical protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MGVQTKQIYRTAIQQICATSETGKAMDKHTEPAEVKNVDASKAETQNPDENPVPETTVVDELTKKYDELNDKHLRLAAEF
ENYKKRAKRDQESAVRYANEKFALDIIDVLDNFERALKSDDENLRDGLEQIHKLYLSILSRNGIEPMKITGTTFDPAFHE
AVACIPADAPEGAIIDVAVPGYMIRDKVLRHAKVAVAKKKE

Sequences:

>Translated_201_residues
MGVQTKQIYRTAIQQICATSETGKAMDKHTEPAEVKNVDASKAETQNPDENPVPETTVVDELTKKYDELNDKHLRLAAEF
ENYKKRAKRDQESAVRYANEKFALDIIDVLDNFERALKSDDENLRDGLEQIHKLYLSILSRNGIEPMKITGTTFDPAFHE
AVACIPADAPEGAIIDVAVPGYMIRDKVLRHAKVAVAKKKE
>Mature_200_residues
GVQTKQIYRTAIQQICATSETGKAMDKHTEPAEVKNVDASKAETQNPDENPVPETTVVDELTKKYDELNDKHLRLAAEFE
NYKKRAKRDQESAVRYANEKFALDIIDVLDNFERALKSDDENLRDGLEQIHKLYLSILSRNGIEPMKITGTTFDPAFHEA
VACIPADAPEGAIIDVAVPGYMIRDKVLRHAKVAVAKKKE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=178, Percent_Identity=26.9662921348315, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=185, Percent_Identity=30.8108108108108, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=29.6511627906977, Blast_Score=63, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324806, Length=150, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22583; Mature: 22452

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVQTKQIYRTAIQQICATSETGKAMDKHTEPAEVKNVDASKAETQNPDENPVPETTVVD
CCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
ELTKKYDELNDKHLRLAAEFENYKKRAKRDQESAVRYANEKFALDIIDVLDNFERALKSD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
DENLRDGLEQIHKLYLSILSRNGIEPMKITGTTFDPAFHEAVACIPADAPEGAIIDVAVP
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECC
GYMIRDKVLRHAKVAVAKKKE
CHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVQTKQIYRTAIQQICATSETGKAMDKHTEPAEVKNVDASKAETQNPDENPVPETTVVD
CCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
ELTKKYDELNDKHLRLAAEFENYKKRAKRDQESAVRYANEKFALDIIDVLDNFERALKSD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
DENLRDGLEQIHKLYLSILSRNGIEPMKITGTTFDPAFHEAVACIPADAPEGAIIDVAVP
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECC
GYMIRDKVLRHAKVAVAKKKE
CHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA