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The map label for this gene is rfcS [H]

Identifier: 124485741

GI number: 124485741

Start: 914886

End: 915884

Strand: Reverse

Name: rfcS [H]

Synonym: Mlab_0919

Alternate gene names: 124485741

Gene position: 915884-914886 (Counterclockwise)

Preceding gene: 124485742

Following gene: 124485740

Centisome position: 50.74

GC content: 54.55

Gene sequence:

>999_bases
ATGCTCTGGATTGAAAAATACCGCCCGACCTCGTTTGAAGAGGTGATCGGACAAAACAGTCCGGTAAAGCAGCTCTCTGC
CTACGCAGCTCTCGGCAACCTCCCGCACCTGCTGGTGATCGGGCGAAGCGGCTGCGGGAAGTCCTCCGCTCTTGAGGTCT
TCTCCCGCAGGTTCTACACGGAATCTGCCGCAGAAAACACGACCGTTCTGCCCGTGAGCGTGATGTTCTCGCAAGGGCAC
ACCTATTTTGCCGAGAACGACCGGTTCGCCCATCTGTATCAGAAGAACAAATCCGTTCTCGCCAACTTCAAGTATATCGT
CAGATGGCACGCCTCCTTGAAACCGCTCTCGGCTCCGTTTCGTCTGATCATCTTTGATGGAGCCGGCGATCTGACTAAAG
ATGCCCAGGCTGCCCTTCGCCGGATCATGGAGCAGTACAGTCAGACCTGCCGGTTTATCTTTGTCACCAGCTCTCTTGCC
TCGATCATCGATCCGATCCGATCAAGGTGCGTGCCGCTCTACTTTTTGCCGATCGATGAAGCAACGATGCGGGACTATCT
GAAGCATGTCCTCGAGCAGGAAGGATGCGTTGGTTCGATCTCCGAAGAGAATCTGGATATGCTGATCCTCGGGTCTGCAG
GCGATCTCAGAAAAGCTCTCGTCTGGCTGGAACTCATGGCTCTCCATGATGTCAGTGACCTTTCAGAGCTCATGACGACC
GAGACCGGAGAGCTGTCGGCCGCAGCGGTCCTTGCGGCCGGAAGAGGCGATTCTGCAGCTGCACAGCAGATCGTTGAAAA
TCTCCAGATCGAGTACGGCATGAATGCCGGCGATGTTCTTCGTTCGCTTCGCAGATGCCTTATTGGAAATATTACGCCGG
ACCTTGCTTTGCTCTTTTCCAAAGCAGAGATCACGCTTGTCGAAGGACAGAACGAATATCTCCAGATGAACGCGTTCACT
GCCGAGCTGACCGAGCTCCTTAGCGGTTCTTCCGTCTAA

Upstream 100 bases:

>100_bases
GACCGGCTCAAGGCATCGGGCGAGACCGTTACGATGCCCGAAATCGACGCAAGATATTTCCTCAGCCAGGCTGTAAAGGA
TATTCAGGAATTCCTTTCCC

Downstream 100 bases:

>100_bases
GGAAGGGCAGGATGAACAACTTAAATCCTCTCGAAGCACAACCATATAGTATGAAGATTCTCTTCGTCATTGCATCAGAC
CGTTTCATAGATATGGGATA

Product: replication factor C small subunit 2

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit; afRFC small subunit; afRFCsm [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLWIEKYRPTSFEEVIGQNSPVKQLSAYAALGNLPHLLVIGRSGCGKSSALEVFSRRFYTESAAENTTVLPVSVMFSQGH
TYFAENDRFAHLYQKNKSVLANFKYIVRWHASLKPLSAPFRLIIFDGAGDLTKDAQAALRRIMEQYSQTCRFIFVTSSLA
SIIDPIRSRCVPLYFLPIDEATMRDYLKHVLEQEGCVGSISEENLDMLILGSAGDLRKALVWLELMALHDVSDLSELMTT
ETGELSAAAVLAAGRGDSAAAQQIVENLQIEYGMNAGDVLRSLRRCLIGNITPDLALLFSKAEITLVEGQNEYLQMNAFT
AELTELLSGSSV

Sequences:

>Translated_332_residues
MLWIEKYRPTSFEEVIGQNSPVKQLSAYAALGNLPHLLVIGRSGCGKSSALEVFSRRFYTESAAENTTVLPVSVMFSQGH
TYFAENDRFAHLYQKNKSVLANFKYIVRWHASLKPLSAPFRLIIFDGAGDLTKDAQAALRRIMEQYSQTCRFIFVTSSLA
SIIDPIRSRCVPLYFLPIDEATMRDYLKHVLEQEGCVGSISEENLDMLILGSAGDLRKALVWLELMALHDVSDLSELMTT
ETGELSAAAVLAAGRGDSAAAQQIVENLQIEYGMNAGDVLRSLRRCLIGNITPDLALLFSKAEITLVEGQNEYLQMNAFT
AELTELLSGSSV
>Mature_332_residues
MLWIEKYRPTSFEEVIGQNSPVKQLSAYAALGNLPHLLVIGRSGCGKSSALEVFSRRFYTESAAENTTVLPVSVMFSQGH
TYFAENDRFAHLYQKNKSVLANFKYIVRWHASLKPLSAPFRLIIFDGAGDLTKDAQAALRRIMEQYSQTCRFIFVTSSLA
SIIDPIRSRCVPLYFLPIDEATMRDYLKHVLEQEGCVGSISEENLDMLILGSAGDLRKALVWLELMALHDVSDLSELMTT
ETGELSAAAVLAAGRGDSAAAQQIVENLQIEYGMNAGDVLRSLRRCLIGNITPDLALLFSKAEITLVEGQNEYLQMNAFT
AELTELLSGSSV

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506491, Length=337, Percent_Identity=27.893175074184, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI31881687, Length=337, Percent_Identity=27.893175074184, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI6677723, Length=222, Percent_Identity=29.7297297297297, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31563534, Length=222, Percent_Identity=29.2792792792793, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI194306571, Length=221, Percent_Identity=29.4117647058824, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI194306567, Length=221, Percent_Identity=29.4117647058824, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4506489, Length=235, Percent_Identity=29.7872340425532, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI108773789, Length=235, Percent_Identity=29.7872340425532, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI194306569, Length=105, Percent_Identity=39.0476190476191, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71986063, Length=226, Percent_Identity=30.5309734513274, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17554730, Length=228, Percent_Identity=28.9473684210526, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17541990, Length=350, Percent_Identity=23.7142857142857, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322528, Length=345, Percent_Identity=28.4057971014493, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324478, Length=222, Percent_Identity=27.027027027027, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324039, Length=222, Percent_Identity=30.1801801801802, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319562, Length=253, Percent_Identity=31.2252964426877, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17647857, Length=330, Percent_Identity=27.5757575757576, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921076, Length=235, Percent_Identity=29.3617021276596, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859927, Length=225, Percent_Identity=30.6666666666667, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19921136, Length=232, Percent_Identity=28.8793103448276, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 36718; Mature: 36718

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWIEKYRPTSFEEVIGQNSPVKQLSAYAALGNLPHLLVIGRSGCGKSSALEVFSRRFYT
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
ESAAENTTVLPVSVMFSQGHTYFAENDRFAHLYQKNKSVLANFKYIVRWHASLKPLSAPF
HCCCCCCEEEEEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHEEEEECCCCCCCCCCE
RLIIFDGAGDLTKDAQAALRRIMEQYSQTCRFIFVTSSLASIIDPIRSRCVPLYFLPIDE
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCEEEEEEECCH
ATMRDYLKHVLEQEGCVGSISEENLDMLILGSAGDLRKALVWLELMALHDVSDLSELMTT
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHC
ETGELSAAAVLAAGRGDSAAAQQIVENLQIEYGMNAGDVLRSLRRCLIGNITPDLALLFS
CCCCCHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
KAEITLVEGQNEYLQMNAFTAELTELLSGSSV
CCEEEEEECCCCEEEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLWIEKYRPTSFEEVIGQNSPVKQLSAYAALGNLPHLLVIGRSGCGKSSALEVFSRRFYT
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
ESAAENTTVLPVSVMFSQGHTYFAENDRFAHLYQKNKSVLANFKYIVRWHASLKPLSAPF
HCCCCCCEEEEEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHEEEEECCCCCCCCCCE
RLIIFDGAGDLTKDAQAALRRIMEQYSQTCRFIFVTSSLASIIDPIRSRCVPLYFLPIDE
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCEEEEEEECCH
ATMRDYLKHVLEQEGCVGSISEENLDMLILGSAGDLRKALVWLELMALHDVSDLSELMTT
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHC
ETGELSAAAVLAAGRGDSAAAQQIVENLQIEYGMNAGDVLRSLRRCLIGNITPDLALLFS
CCCCCHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
KAEITLVEGQNEYLQMNAFTAELTELLSGSSV
CCEEEEEECCCCEEEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475; 12384579 [H]