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The map label for this gene is yieO [C]

Identifier: 124485217

GI number: 124485217

Start: 367205

End: 368785

Strand: Reverse

Name: yieO [C]

Synonym: Mlab_0390

Alternate gene names: 124485217

Gene position: 368785-367205 (Counterclockwise)

Preceding gene: 124485218

Following gene: 124485216

Centisome position: 20.43

GC content: 52.18

Gene sequence:

>1581_bases
ATGGAGACATTGCGTCTGAGTGCAAAACAGTATATTATTGCCATAACAGTCGGAATTGCCTGTTTTTTACCCCCGTTCAT
CGGAGAAGCGACAAACATCGCCCTCCCAAATATGCTCGTCGGACTGGGATACACAATGGGAGCCGACTACTGGAATCTTT
ACGGCTGGATCCTGACCGTTTATCTGTTGACATCCACGATCTTTCTTATACCTGCTGCAAGACTTGCCGATAAATTCAGC
AAGAAGTGGTTCTTCAGTATCGGTGTCCTTCTTCTTGGGGTCGGTTCTCTAGGTGTTGGAATGTCGACTTCCGGCGAAAT
GGTCCTTGCCATGCGCACGATCGAGGGTATCGGAAACGCACTGATGTTTGGAAGCGCGATAGCTTTGCTCGCATCGGCGG
TAAAAGTAGAACTCCGGGGGACCGCAATTGGTATCGCGATGACCGGCGTATTCCTTGGCCAGCTCGCCGGACCGCTGCTT
GCCGGCGCTCTGACGGATGTCTTTGGATGGTCGATGGTGTATCTTATCCTCTGTCCGGTGGCACTTCTCTCCTTCTTCCT
TGCCATTCCTTTCATCCCGCGGGATCAGCCAACCGACAAAGGACCGTACGACTGGATCGGTGCCCTGCTCTTTATGGGAG
GAATGGGTTTTGCCCTCTACGGATTTTCCAAACAGCCGAACACGGAGGCACTCGCACTTCTCGTTTCCGGGATCTTTCTT
TTGATCGCATTCTTTTTCTATGAAAAGAGGAATAAAAATCCCCTCATCCCGGTCAAACTGATTCTCCGCAACAGAGGATT
CACCTTCAATAACTCCGCAAACCTGCTGTATTATGTGGCGATCTATGCAATGGGCTCCCTTGTTTCGCTCTACATGACGA
ATGTCTGGGGGATCACGGACGCACTGCCCCGTGCGCTGATCGTGACAACGCAAGGGTTGATTCTCGTGCTCTTTACGATC
GTTGCCGGCAGATTCTACGACCACCTTCTGCCCAGATGGCTGCTCGCATTCGGCGTACTTTTCACGATCGGCGGCATAGC
CGGAGCATGGATAATGGGTGTTCCCTCAACGGGAACCCTCTGGCTGATTGGAGCGATCCTCTGCGCAAGCGTCGCGGCAT
TCGGTCTCGGATCTTTAATCCTCGCCGGGATCGACCGGTATATCAAAAATGCTCCGCCAAAGTATGCAACGACAACGGGA
CTTATCCTCATCAGTATTGGAATGATCATCCTTCTTACCTGCGGGACCGAAACCGCGATCTGGAGCATGATCGCCGTCCA
GGCATTCTTCGGAGTAGGTATCGCCATATTCGTCACGCCGAACAGCACGGCGATCATGAACTCCGTGACGCCTCAGGAAT
TTGGGATGGCGTCCGGAACCCTTTCTACGACCAGAATGCTTGGCATGGCGATCTCGATTGGGATCGTGTCCATTCTGAAA
AACATCTTCCTTTCAGGGACATCTGACGACTATGCGGCCTCCTTCCTCCAGATGATGGACGGGACCATTATTGCTGCACT
CGTCGTTTTAGTTCTCGCGATGATCTTTTCCTGGACCGCAGGGTCGAGAAGGAGAATATAA

Upstream 100 bases:

>100_bases
AAGGACGTAGACATTACTAGTTAAATCAATTTAACTCATTTTTGAGTTATAACTCAAAACAGGCAACGATTAATACCTCA
AAAATCCATACTGAGGTATT

Downstream 100 bases:

>100_bases
TCCAAAATCCAACAACCACTCACTTTTTTTCCTGCCGCCTTCATAGTATTACCTCATATGGCAAAGAGATCCGGCAAAAG
AAAACGTTCGTCCAAAACGG

Product: hypothetical protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
METLRLSAKQYIIAITVGIACFLPPFIGEATNIALPNMLVGLGYTMGADYWNLYGWILTVYLLTSTIFLIPAARLADKFS
KKWFFSIGVLLLGVGSLGVGMSTSGEMVLAMRTIEGIGNALMFGSAIALLASAVKVELRGTAIGIAMTGVFLGQLAGPLL
AGALTDVFGWSMVYLILCPVALLSFFLAIPFIPRDQPTDKGPYDWIGALLFMGGMGFALYGFSKQPNTEALALLVSGIFL
LIAFFFYEKRNKNPLIPVKLILRNRGFTFNNSANLLYYVAIYAMGSLVSLYMTNVWGITDALPRALIVTTQGLILVLFTI
VAGRFYDHLLPRWLLAFGVLFTIGGIAGAWIMGVPSTGTLWLIGAILCASVAAFGLGSLILAGIDRYIKNAPPKYATTTG
LILISIGMIILLTCGTETAIWSMIAVQAFFGVGIAIFVTPNSTAIMNSVTPQEFGMASGTLSTTRMLGMAISIGIVSILK
NIFLSGTSDDYAASFLQMMDGTIIAALVVLVLAMIFSWTAGSRRRI

Sequences:

>Translated_526_residues
METLRLSAKQYIIAITVGIACFLPPFIGEATNIALPNMLVGLGYTMGADYWNLYGWILTVYLLTSTIFLIPAARLADKFS
KKWFFSIGVLLLGVGSLGVGMSTSGEMVLAMRTIEGIGNALMFGSAIALLASAVKVELRGTAIGIAMTGVFLGQLAGPLL
AGALTDVFGWSMVYLILCPVALLSFFLAIPFIPRDQPTDKGPYDWIGALLFMGGMGFALYGFSKQPNTEALALLVSGIFL
LIAFFFYEKRNKNPLIPVKLILRNRGFTFNNSANLLYYVAIYAMGSLVSLYMTNVWGITDALPRALIVTTQGLILVLFTI
VAGRFYDHLLPRWLLAFGVLFTIGGIAGAWIMGVPSTGTLWLIGAILCASVAAFGLGSLILAGIDRYIKNAPPKYATTTG
LILISIGMIILLTCGTETAIWSMIAVQAFFGVGIAIFVTPNSTAIMNSVTPQEFGMASGTLSTTRMLGMAISIGIVSILK
NIFLSGTSDDYAASFLQMMDGTIIAALVVLVLAMIFSWTAGSRRRI
>Mature_526_residues
METLRLSAKQYIIAITVGIACFLPPFIGEATNIALPNMLVGLGYTMGADYWNLYGWILTVYLLTSTIFLIPAARLADKFS
KKWFFSIGVLLLGVGSLGVGMSTSGEMVLAMRTIEGIGNALMFGSAIALLASAVKVELRGTAIGIAMTGVFLGQLAGPLL
AGALTDVFGWSMVYLILCPVALLSFFLAIPFIPRDQPTDKGPYDWIGALLFMGGMGFALYGFSKQPNTEALALLVSGIFL
LIAFFFYEKRNKNPLIPVKLILRNRGFTFNNSANLLYYVAIYAMGSLVSLYMTNVWGITDALPRALIVTTQGLILVLFTI
VAGRFYDHLLPRWLLAFGVLFTIGGIAGAWIMGVPSTGTLWLIGAILCASVAAFGLGSLILAGIDRYIKNAPPKYATTTG
LILISIGMIILLTCGTETAIWSMIAVQAFFGVGIAIFVTPNSTAIMNSVTPQEFGMASGTLSTTRMLGMAISIGIVSILK
NIFLSGTSDDYAASFLQMMDGTIIAALVVLVLAMIFSWTAGSRRRI

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=265, Percent_Identity=29.4339622641509, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081983, Length=300, Percent_Identity=26, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56340; Mature: 56340

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLRLSAKQYIIAITVGIACFLPPFIGEATNIALPNMLVGLGYTMGADYWNLYGWILTV
CCCCHHCHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHH
YLLTSTIFLIPAARLADKFSKKWFFSIGVLLLGVGSLGVGMSTSGEMVLAMRTIEGIGNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHH
LMFGSAIALLASAVKVELRGTAIGIAMTGVFLGQLAGPLLAGALTDVFGWSMVYLILCPV
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSFFLAIPFIPRDQPTDKGPYDWIGALLFMGGMGFALYGFSKQPNTEALALLVSGIFL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
LIAFFFYEKRNKNPLIPVKLILRNRGFTFNNSANLLYYVAIYAMGSLVSLYMTNVWGITD
HHHHHHHHHCCCCCCCHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPRALIVTTQGLILVLFTIVAGRFYDHLLPRWLLAFGVLFTIGGIAGAWIMGVPSTGTL
HHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
WLIGAILCASVAAFGLGSLILAGIDRYIKNAPPKYATTTGLILISIGMIILLTCGTETAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCHHHH
WSMIAVQAFFGVGIAIFVTPNSTAIMNSVTPQEFGMASGTLSTTRMLGMAISIGIVSILK
HHHHHHHHHHHCCEEEEECCCCCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NIFLSGTSDDYAASFLQMMDGTIIAALVVLVLAMIFSWTAGSRRRI
HHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
METLRLSAKQYIIAITVGIACFLPPFIGEATNIALPNMLVGLGYTMGADYWNLYGWILTV
CCCCHHCHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHH
YLLTSTIFLIPAARLADKFSKKWFFSIGVLLLGVGSLGVGMSTSGEMVLAMRTIEGIGNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHH
LMFGSAIALLASAVKVELRGTAIGIAMTGVFLGQLAGPLLAGALTDVFGWSMVYLILCPV
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSFFLAIPFIPRDQPTDKGPYDWIGALLFMGGMGFALYGFSKQPNTEALALLVSGIFL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
LIAFFFYEKRNKNPLIPVKLILRNRGFTFNNSANLLYYVAIYAMGSLVSLYMTNVWGITD
HHHHHHHHHCCCCCCCHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPRALIVTTQGLILVLFTIVAGRFYDHLLPRWLLAFGVLFTIGGIAGAWIMGVPSTGTL
HHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
WLIGAILCASVAAFGLGSLILAGIDRYIKNAPPKYATTTGLILISIGMIILLTCGTETAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCHHHH
WSMIAVQAFFGVGIAIFVTPNSTAIMNSVTPQEFGMASGTLSTTRMLGMAISIGIVSILK
HHHHHHHHHHHCCEEEEECCCCCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NIFLSGTSDDYAASFLQMMDGTIIAALVVLVLAMIFSWTAGSRRRI
HHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]