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The map label for this gene is fen

Identifier: 124485183

GI number: 124485183

Start: 335631

End: 336632

Strand: Reverse

Name: fen

Synonym: Mlab_0356

Alternate gene names: 124485183

Gene position: 336632-335631 (Counterclockwise)

Preceding gene: 124485184

Following gene: 124485181

Centisome position: 18.65

GC content: 53.69

Gene sequence:

>1002_bases
ATGGGTGTTGCCCTCCGGCCGCTTCTTGCGGAGTATAAGGAGTCTCCCGGATGGGACGGGCTTGGCGGGATTGCAGCAGT
CGACGCATTCAATGCTCTCTATCAGTTCCTTTCCGGGATACGGCAGGCCGACGGCGCACCCCTAATGGATGGGCAGGGGC
GTATCACCTCGCATCTGAGCGGACTTCTCTTTCGGAATGCGAACCTGATTGAAAAAAATATCACCCCCATATACGTTTTT
GACGGAAAGCCGCCGGTCTTCAAAGCATCAACACTCTCAAAGCGGCGCGAAGTTCGGGAAAACGCAGCTGATGCATGGGA
AAAAGCCCTGAAAGAGGGGGATGAAGAGAGCGCACGAAAATATGCAATGGCCTCTTCGAAAATCGATGCCTTCATCATTG
ATTCATCGAAGGAACTCCTCTCTGCCCTTGGGATAGCCTGGATACAGGCACCCGAAGAGGGGGAGGCCCAGTCTTCGTTT
ATGACAAAGAATGGGGACGTGACCTACGCCGTTTCTCAGGATTATGACTCACTCCTGTTCGGAGCACCGGACCTGGTTCG
AAACATCACGGTCTCGGGCAAGAAAAGGATTCGGGGAAAGGTACTGTCAGTGTATCCTGAGCGACTGAGACTGGAGGAGG
TACTCACTGGACTTTCCGTAACTCAGGAAGAACTCATCCAGATAGCTCTACTGATTGGGACCGATTACAACAGCGGGGTG
CCGGGCGTCGGGCCAAAGACCGCTGTCAAGATCGTCAGAGAGGGAAAGTTCTATGATCGGATCGGAGAGTCGGAGAATGC
CGCAGAGCCAGATCAGCTGATCGGGTACTTTATGGATCCTCCGGTCGAGCGGTCCTATAATATTGAAAGTAGGTCACCAG
ATCCGGATCGGGTCATTGATCTATTGTGCGGGGAGCATGGTTTTACTCAGGAAAGAGTTGAGGCCGGACTCGAACGGCTC
GGCGCGAAAAAGGGTCAGGCAACTCTGGACGCCTGGTTCTAA

Upstream 100 bases:

>100_bases
TCTTGAAGACCTAGGCAGACAGCTGATCGCCAAGCAGGACCACACCTCACAGACGGTCATCGAAGCGGTCGAAAGTATCA
AAATGCGGATGTACCGTTAG

Downstream 100 bases:

>100_bases
ATCACATTTTTGAATGATATAAATAGGTTTTTCAGTAGAGGTATTTCTAAAAAAATGAAAATAATCAGGCGCTTTTACCG
ACGATGATAAGCGTCTTTTC

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MGVALRPLLAEYKESPGWDGLGGIAAVDAFNALYQFLSGIRQADGAPLMDGQGRITSHLSGLLFRNANLIEKNITPIYVF
DGKPPVFKASTLSKRREVRENAADAWEKALKEGDEESARKYAMASSKIDAFIIDSSKELLSALGIAWIQAPEEGEAQSSF
MTKNGDVTYAVSQDYDSLLFGAPDLVRNITVSGKKRIRGKVLSVYPERLRLEEVLTGLSVTQEELIQIALLIGTDYNSGV
PGVGPKTAVKIVREGKFYDRIGESENAAEPDQLIGYFMDPPVERSYNIESRSPDPDRVIDLLCGEHGFTQERVEAGLERL
GAKKGQATLDAWF

Sequences:

>Translated_333_residues
MGVALRPLLAEYKESPGWDGLGGIAAVDAFNALYQFLSGIRQADGAPLMDGQGRITSHLSGLLFRNANLIEKNITPIYVF
DGKPPVFKASTLSKRREVRENAADAWEKALKEGDEESARKYAMASSKIDAFIIDSSKELLSALGIAWIQAPEEGEAQSSF
MTKNGDVTYAVSQDYDSLLFGAPDLVRNITVSGKKRIRGKVLSVYPERLRLEEVLTGLSVTQEELIQIALLIGTDYNSGV
PGVGPKTAVKIVREGKFYDRIGESENAAEPDQLIGYFMDPPVERSYNIESRSPDPDRVIDLLCGEHGFTQERVEAGLERL
GAKKGQATLDAWF
>Mature_332_residues
GVALRPLLAEYKESPGWDGLGGIAAVDAFNALYQFLSGIRQADGAPLMDGQGRITSHLSGLLFRNANLIEKNITPIYVFD
GKPPVFKASTLSKRREVRENAADAWEKALKEGDEESARKYAMASSKIDAFIIDSSKELLSALGIAWIQAPEEGEAQSSFM
TKNGDVTYAVSQDYDSLLFGAPDLVRNITVSGKKRIRGKVLSVYPERLRLEEVLTGLSVTQEELIQIALLIGTDYNSGVP
GVGPKTAVKIVREGKFYDRIGESENAAEPDQLIGYFMDPPVERSYNIESRSPDPDRVIDLLCGEHGFTQERVEAGLERLG
AKKGQATLDAWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=330, Percent_Identity=33.6363636363636, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI194018535, Length=225, Percent_Identity=26.2222222222222, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194018531, Length=225, Percent_Identity=26.2222222222222, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI51988900, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510005, Length=326, Percent_Identity=35.5828220858896, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322736, Length=332, Percent_Identity=34.0361445783133, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324607, Length=242, Percent_Identity=26.8595041322314, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321697, Length=222, Percent_Identity=28.3783783783784, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320469, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17647423, Length=335, Percent_Identity=32.2388059701493, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24658219, Length=185, Percent_Identity=28.6486486486486, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137168, Length=234, Percent_Identity=25.2136752136752, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706854, Length=143, Percent_Identity=32.1678321678322, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706850, Length=143, Percent_Identity=32.1678321678322, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706852, Length=143, Percent_Identity=32.1678321678322, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_METLZ (A2SQC6)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029799.1
- ProteinModelPortal:   A2SQC6
- SMR:   A2SQC6
- STRING:   A2SQC6
- GeneID:   4794968
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0356
- eggNOG:   arNOG04505
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- PhylomeDB:   A2SQC6
- ProtClustDB:   PRK03980
- BioCyc:   MLAB410358:MLAB_0356-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 36493; Mature: 36361

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVALRPLLAEYKESPGWDGLGGIAAVDAFNALYQFLSGIRQADGAPLMDGQGRITSHLS
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GLLFRNANLIEKNITPIYVFDGKPPVFKASTLSKRREVRENAADAWEKALKEGDEESARK
HHHHCCCHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YAMASSKIDAFIIDSSKELLSALGIAWIQAPEEGEAQSSFMTKNGDVTYAVSQDYDSLLF
HHHHHCCCCEEEECCHHHHHHHHHHHHEECCCCCCHHHHHEECCCCEEEEECCCCHHHHH
GAPDLVRNITVSGKKRIRGKVLSVYPERLRLEEVLTGLSVTQEELIQIALLIGTDYNSGV
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCC
PGVGPKTAVKIVREGKFYDRIGESENAAEPDQLIGYFMDPPVERSYNIESRSPDPDRVID
CCCCHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
LLCGEHGFTQERVEAGLERLGAKKGQATLDAWF
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GVALRPLLAEYKESPGWDGLGGIAAVDAFNALYQFLSGIRQADGAPLMDGQGRITSHLS
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GLLFRNANLIEKNITPIYVFDGKPPVFKASTLSKRREVRENAADAWEKALKEGDEESARK
HHHHCCCHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YAMASSKIDAFIIDSSKELLSALGIAWIQAPEEGEAQSSFMTKNGDVTYAVSQDYDSLLF
HHHHHCCCCEEEECCHHHHHHHHHHHHEECCCCCCHHHHHEECCCCEEEEECCCCHHHHH
GAPDLVRNITVSGKKRIRGKVLSVYPERLRLEEVLTGLSVTQEELIQIALLIGTDYNSGV
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCC
PGVGPKTAVKIVREGKFYDRIGESENAAEPDQLIGYFMDPPVERSYNIESRSPDPDRVID
CCCCHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
LLCGEHGFTQERVEAGLERLGAKKGQATLDAWF
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA