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The map label for this gene is yhcA [H]

Identifier: 124485181

GI number: 124485181

Start: 333349

End: 334803

Strand: Reverse

Name: yhcA [H]

Synonym: Mlab_0354

Alternate gene names: 124485181

Gene position: 334803-333349 (Counterclockwise)

Preceding gene: 124485183

Following gene: 124485180

Centisome position: 18.55

GC content: 50.03

Gene sequence:

>1455_bases
ATGAAAGATCCTCTCCCAACTGGAAAAACCCTGATCTTCATCCTTATTGTTGCGTCCATCGCCTCGTTTATGTCATCCCT
TGACGGAACGATCGTCAATATCGCTCTCCCAAGCATTGGGGAAGCTTTCGATCTCAGCACATCTTCGGTTTCGTGGGTCA
GTACCATATATCTACTCGTCATCGCCGGTCTGCTCCTGATCATCGGTAAAATTATCGACACAATCGGCCTCAAAAAAATC
TTTCTGGGAGGGTTTGCCATATTTGTAATCGGCTCTTTTTCCTGCGGATTTTTCCCCGAACTCCTGCACTCGTTCAACCT
CCTCCTGATCTCACGTGTCGTTCAGGCAATAGGCGGCGGAATGATGATAGTAGTGGCACCTGCCATGCTTTCCCTGTTCA
TGCCGGGGGACAGACGGGCAAAAGGCCTCTCACTCATAATGCTTTTTGCCGCCGTTGGGATGGCACTTGGTCCGACTCTT
GGAGGATATCTTACCGAGTACCTTTCCTGGCACTGGATCTTTTTCATCAACGTCCCGGTCGGCATTTTTGCCATTATTCT
GGGCCTGATCGTCATCCCAGACAACAAACCCGATTCGTGCCCTCTCAAAAGATTTGACACACCCGGGGCAATCCTTATTT
TTGTCGGCCTTGCGGCGCTGCTCTTCGCCTTCTCGGAAGGATTTTCCCTTGGCTGGACCTCGCCACAGATCATCGTCTCG
GTCCTCCTTGCGGTGATCTTTATCGGCGGTTTCATCCGACGTGAACTGCACTACGACAACCCGCTGATCGACCTTGGAAT
GTTCAAAAGCAGATCGTTTGTTGTCGTGAACATTGTCTTCGCACTGCTCTATTTCACCTTTGCCGGAGCGAACTATGTTC
TCCCGTTCTACCTTGAGTATATACACGGTTTTTCGACCGCAAACGCGGGACTCATCCTTACAAGCCTCTCGGTCGGGATG
ATGATCACCGGTATTTTATCCGGTCTGATCTATGCAAAACTGGTCGGAAGAATAAAATATATGGTCATGGCAGGTGTTGT
TCTGGTTGGCGTGGGATATTTCCTTCTCTCTCACTTGAGCCCGGTCACAGGGCTTGGGATCATAATCACCGCACTTTCCA
TGATCGGTCTCGGCATTGGTGTGACGGTCACGCCTCTTACCACGCTTATCATGGGGGCAGCACCCCCATCCAAGCAGGGA
ATGGTCTCAAGTCTGACAGGTCTTGAGCGCTATGCCCCCATGACGATCGGTGTGGCAGTCTACAACCTGATGGTGATTGT
TGGGATCGTCATAAGCGTCAGGGACTCGGGGATTATCTATAAAACACCAGCAGACATCAGCCCATATATCCTCTCCTTCG
GCTTTGACCTGGCATTTGTGATTTCGGTGATTTTGGCCTTGATAATTCTGGCTCTCTGCTTCTTTATCAAAGAAGAAAAA
GCGGCAGAAGAGTAA

Upstream 100 bases:

>100_bases
ATCCATTATGCTCACAAATATGCATGACATCCTAATAATGACTTTGGATAAGCATCTACCCTATTGATTTATAACAAAGA
AAATCCTTTTTTTATCATCT

Downstream 100 bases:

>100_bases
TCCTTTTTTCTCATCAAACATAAATAGTTGCAAAAGCAACCAAATAATGTCAATGCAGCAAAACACATTACAGAGAGGTC
TTGGCCACAACATCATGGCC

Product: indolepyruvate ferredoxin oxidoreductase alpha and beta subunits-like protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKDPLPTGKTLIFILIVASIASFMSSLDGTIVNIALPSIGEAFDLSTSSVSWVSTIYLLVIAGLLLIIGKIIDTIGLKKI
FLGGFAIFVIGSFSCGFFPELLHSFNLLLISRVVQAIGGGMMIVVAPAMLSLFMPGDRRAKGLSLIMLFAAVGMALGPTL
GGYLTEYLSWHWIFFINVPVGIFAIILGLIVIPDNKPDSCPLKRFDTPGAILIFVGLAALLFAFSEGFSLGWTSPQIIVS
VLLAVIFIGGFIRRELHYDNPLIDLGMFKSRSFVVVNIVFALLYFTFAGANYVLPFYLEYIHGFSTANAGLILTSLSVGM
MITGILSGLIYAKLVGRIKYMVMAGVVLVGVGYFLLSHLSPVTGLGIIITALSMIGLGIGVTVTPLTTLIMGAAPPSKQG
MVSSLTGLERYAPMTIGVAVYNLMVIVGIVISVRDSGIIYKTPADISPYILSFGFDLAFVISVILALIILALCFFIKEEK
AAEE

Sequences:

>Translated_484_residues
MKDPLPTGKTLIFILIVASIASFMSSLDGTIVNIALPSIGEAFDLSTSSVSWVSTIYLLVIAGLLLIIGKIIDTIGLKKI
FLGGFAIFVIGSFSCGFFPELLHSFNLLLISRVVQAIGGGMMIVVAPAMLSLFMPGDRRAKGLSLIMLFAAVGMALGPTL
GGYLTEYLSWHWIFFINVPVGIFAIILGLIVIPDNKPDSCPLKRFDTPGAILIFVGLAALLFAFSEGFSLGWTSPQIIVS
VLLAVIFIGGFIRRELHYDNPLIDLGMFKSRSFVVVNIVFALLYFTFAGANYVLPFYLEYIHGFSTANAGLILTSLSVGM
MITGILSGLIYAKLVGRIKYMVMAGVVLVGVGYFLLSHLSPVTGLGIIITALSMIGLGIGVTVTPLTTLIMGAAPPSKQG
MVSSLTGLERYAPMTIGVAVYNLMVIVGIVISVRDSGIIYKTPADISPYILSFGFDLAFVISVILALIILALCFFIKEEK
AAEE
>Mature_484_residues
MKDPLPTGKTLIFILIVASIASFMSSLDGTIVNIALPSIGEAFDLSTSSVSWVSTIYLLVIAGLLLIIGKIIDTIGLKKI
FLGGFAIFVIGSFSCGFFPELLHSFNLLLISRVVQAIGGGMMIVVAPAMLSLFMPGDRRAKGLSLIMLFAAVGMALGPTL
GGYLTEYLSWHWIFFINVPVGIFAIILGLIVIPDNKPDSCPLKRFDTPGAILIFVGLAALLFAFSEGFSLGWTSPQIIVS
VLLAVIFIGGFIRRELHYDNPLIDLGMFKSRSFVVVNIVFALLYFTFAGANYVLPFYLEYIHGFSTANAGLILTSLSVGM
MITGILSGLIYAKLVGRIKYMVMAGVVLVGVGYFLLSHLSPVTGLGIIITALSMIGLGIGVTVTPLTTLIMGAAPPSKQG
MVSSLTGLERYAPMTIGVAVYNLMVIVGIVISVRDSGIIYKTPADISPYILSFGFDLAFVISVILALIILALCFFIKEEK
AAEE

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=432, Percent_Identity=29.6296296296296, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=30, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=426, Percent_Identity=28.8732394366197, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790195, Length=418, Percent_Identity=28.4688995215311, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788392, Length=305, Percent_Identity=32.7868852459016, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=29.5180722891566, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=390, Percent_Identity=27.4358974358974, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=419, Percent_Identity=26.7303102625298, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=322, Percent_Identity=26.7080745341615, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=30.5194805194805, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=151, Percent_Identity=27.1523178807947, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=295, Percent_Identity=25.4237288135593, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51746; Mature: 51746

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDPLPTGKTLIFILIVASIASFMSSLDGTIVNIALPSIGEAFDLSTSSVSWVSTIYLLV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
IAGLLLIIGKIIDTIGLKKIFLGGFAIFVIGSFSCGFFPELLHSFNLLLISRVVQAIGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
MMIVVAPAMLSLFMPGDRRAKGLSLIMLFAAVGMALGPTLGGYLTEYLSWHWIFFINVPV
EEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCH
GIFAIILGLIVIPDNKPDSCPLKRFDTPGAILIFVGLAALLFAFSEGFSLGWTSPQIIVS
HHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VLLAVIFIGGFIRRELHYDNPLIDLGMFKSRSFVVVNIVFALLYFTFAGANYVLPFYLEY
HHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IHGFSTANAGLILTSLSVGMMITGILSGLIYAKLVGRIKYMVMAGVVLVGVGYFLLSHLS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVTGLGIIITALSMIGLGIGVTVTPLTTLIMGAAPPSKQGMVSSLTGLERYAPMTIGVAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
YNLMVIVGIVISVRDSGIIYKTPADISPYILSFGFDLAFVISVILALIILALCFFIKEEK
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAEE
CCCC
>Mature Secondary Structure
MKDPLPTGKTLIFILIVASIASFMSSLDGTIVNIALPSIGEAFDLSTSSVSWVSTIYLLV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
IAGLLLIIGKIIDTIGLKKIFLGGFAIFVIGSFSCGFFPELLHSFNLLLISRVVQAIGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
MMIVVAPAMLSLFMPGDRRAKGLSLIMLFAAVGMALGPTLGGYLTEYLSWHWIFFINVPV
EEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCH
GIFAIILGLIVIPDNKPDSCPLKRFDTPGAILIFVGLAALLFAFSEGFSLGWTSPQIIVS
HHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VLLAVIFIGGFIRRELHYDNPLIDLGMFKSRSFVVVNIVFALLYFTFAGANYVLPFYLEY
HHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IHGFSTANAGLILTSLSVGMMITGILSGLIYAKLVGRIKYMVMAGVVLVGVGYFLLSHLS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVTGLGIIITALSMIGLGIGVTVTPLTTLIMGAAPPSKQGMVSSLTGLERYAPMTIGVAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
YNLMVIVGIVISVRDSGIIYKTPADISPYILSFGFDLAFVISVILALIILALCFFIKEEK
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAEE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]