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The map label for this gene is kamA [H]

Identifier: 124485172

GI number: 124485172

Start: 325275

End: 326636

Strand: Reverse

Name: kamA [H]

Synonym: Mlab_0345

Alternate gene names: 124485172

Gene position: 326636-325275 (Counterclockwise)

Preceding gene: 124485173

Following gene: 124485171

Centisome position: 18.1

GC content: 46.99

Gene sequence:

>1362_bases
ATGACACTCCAGACAGAGTCTCTGGAGAAAACCGCAGACACAGAGACGCAAACGAACGAGAGTGGTGCCTTTTCAAAGGG
AAGATACGCCGGGATTGATGACGGTGAATACTGGAAAAACTGGAAATGGCAGGTCAGGCATGCGGTCAGAAGTATCGACA
TGGTCCAGCAGGTACTGGGAATAACGTTTGATCCAAAAGAACGAGAAGAACTCCAGAGAACTGTAGAGAAGTTCCCAATC
AGTATCACCCCATACTACCTCTCGCTTATCGATACCGAGGATTACCGAAATGACCCGATTTTCAGGCAGGCATTCCCATC
TCCTGCAGAACTCATCGTAGAAAATTATGAGCTCAGTGATCCGCTTGCAGAAGATAAAGACAGTCCATGCGAATGTATCA
CCCACAGGTATCCGGATCGAGTTCTGTTTCTTGTCAGTAATACCTGTGCAATGTACTGCAGACACTGTACACGGAAACGA
AAAGTAGGTGATAAAGATTCCATCCCAGACCGTGAAAAAATCCTTGAAGGCATTGCCTACATACGTGAAAACACCCAGAT
TCGCGACGTACTCCTTTCAGGAGGAGATCCGTTTATGTTGAGTGATGAGAGCTTGGACTGGATACTTACCGAACTTACCG
CCATCCCGCATGTAGAAGTGATACGAATCGGGACAAGAGTTCCTGTCGTCCTCCCGTTTAGGATCACCAATCAGCTGGTC
GACATTCTCAAAAAGCACAAACCGATCTGGATCAATACACAGTTCAATCACCCAAAAGAGATGACTCCGTCAGCCCAGGC
AGCAGTTGCAAAACTTGTCGATGCCGGAATTCCGCTTGGAAATCAGTCGGTCCTCCTTGCACGGATTAACGACTGCCCGG
TCATCATGAAAGAACTGGTACATCAACTGGTGAAAAACCGAATTCGGCCTTATTATTTGTATCAGTGCGATCTGTCTGAA
GGAATCAGTCATTTCCGAACACCCATCGCGAAAGGAATCGAGATCATGGAAAGTCTGATCGGACACACCAGCGGATTTGC
CGTCCCAAGATATGTGGTGGATGCGCCGGGAGGAGGAGGGAAAATCCCTGTGTCTCCAAATTATCTCTTGACATGGTCGG
TAAACAAAGTAGTTCTTAGAAACTATGAAGGAATCATCTGCACATATCAGGAGCCGGCGGTTTATGACAGAATTATCTGT
GATCAGAATTGCGATGCCTGCAAACTCCAGCTAAACATACGAAATGCAGATGAATCGAAGGTGGTCGGCGTCGCAAAGCT
TCTCGCCGATTATGATGATACAACTACACTGATCCCGGAAAATAACGAACGTCTTGAAAGGAGGGAGGATGAAACCAACT
GA

Upstream 100 bases:

>100_bases
ATGAAAACAATCTGCGAATAAATCCGATCACCGGATGGATCATGGATCATCATTTTGAATTAAGTGATTCCGGCCCGACC
AAAACCGGGGAGCGTGATAT

Downstream 100 bases:

>100_bases
TTCCGTGACCACATATGGAAAAAGTATACTTCAGCACGGCCCTTTCAACAACAGGATCTACTTCATGACACTTGCACCAG
ATGACCTGCCAGGGATCATC

Product: L-lysine 2,3-aminomutase

Products: NA

Alternate protein names: LAM; KAM [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTLQTESLEKTADTETQTNESGAFSKGRYAGIDDGEYWKNWKWQVRHAVRSIDMVQQVLGITFDPKEREELQRTVEKFPI
SITPYYLSLIDTEDYRNDPIFRQAFPSPAELIVENYELSDPLAEDKDSPCECITHRYPDRVLFLVSNTCAMYCRHCTRKR
KVGDKDSIPDREKILEGIAYIRENTQIRDVLLSGGDPFMLSDESLDWILTELTAIPHVEVIRIGTRVPVVLPFRITNQLV
DILKKHKPIWINTQFNHPKEMTPSAQAAVAKLVDAGIPLGNQSVLLARINDCPVIMKELVHQLVKNRIRPYYLYQCDLSE
GISHFRTPIAKGIEIMESLIGHTSGFAVPRYVVDAPGGGGKIPVSPNYLLTWSVNKVVLRNYEGIICTYQEPAVYDRIIC
DQNCDACKLQLNIRNADESKVVGVAKLLADYDDTTTLIPENNERLERREDETN

Sequences:

>Translated_453_residues
MTLQTESLEKTADTETQTNESGAFSKGRYAGIDDGEYWKNWKWQVRHAVRSIDMVQQVLGITFDPKEREELQRTVEKFPI
SITPYYLSLIDTEDYRNDPIFRQAFPSPAELIVENYELSDPLAEDKDSPCECITHRYPDRVLFLVSNTCAMYCRHCTRKR
KVGDKDSIPDREKILEGIAYIRENTQIRDVLLSGGDPFMLSDESLDWILTELTAIPHVEVIRIGTRVPVVLPFRITNQLV
DILKKHKPIWINTQFNHPKEMTPSAQAAVAKLVDAGIPLGNQSVLLARINDCPVIMKELVHQLVKNRIRPYYLYQCDLSE
GISHFRTPIAKGIEIMESLIGHTSGFAVPRYVVDAPGGGGKIPVSPNYLLTWSVNKVVLRNYEGIICTYQEPAVYDRIIC
DQNCDACKLQLNIRNADESKVVGVAKLLADYDDTTTLIPENNERLERREDETN
>Mature_452_residues
TLQTESLEKTADTETQTNESGAFSKGRYAGIDDGEYWKNWKWQVRHAVRSIDMVQQVLGITFDPKEREELQRTVEKFPIS
ITPYYLSLIDTEDYRNDPIFRQAFPSPAELIVENYELSDPLAEDKDSPCECITHRYPDRVLFLVSNTCAMYCRHCTRKRK
VGDKDSIPDREKILEGIAYIRENTQIRDVLLSGGDPFMLSDESLDWILTELTAIPHVEVIRIGTRVPVVLPFRITNQLVD
ILKKHKPIWINTQFNHPKEMTPSAQAAVAKLVDAGIPLGNQSVLLARINDCPVIMKELVHQLVKNRIRPYYLYQCDLSEG
ISHFRTPIAKGIEIMESLIGHTSGFAVPRYVVDAPGGGGKIPVSPNYLLTWSVNKVVLRNYEGIICTYQEPAVYDRIICD
QNCDACKLQLNIRNADESKVVGVAKLLADYDDTTTLIPENNERLERREDETN

Specific function: Catalyzes the interconversion of L-alpha-lysine and L- beta-lysine [H]

COG id: COG1509

COG function: function code E; Lysine 2,3-aminomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KamA family [H]

Homologues:

Organism=Escherichia coli, GI1790589, Length=329, Percent_Identity=32.2188449848024, Blast_Score=171, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003739
- InterPro:   IPR022459
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =5.4.3.2 [H]

Molecular weight: Translated: 51633; Mature: 51502

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQTESLEKTADTETQTNESGAFSKGRYAGIDDGEYWKNWKWQVRHAVRSIDMVQQVLG
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHC
ITFDPKEREELQRTVEKFPISITPYYLSLIDTEDYRNDPIFRQAFPSPAELIVENYELSD
CCCCCHHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCCHHHCCCCHHHHHEECCCCCC
PLAEDKDSPCECITHRYPDRVLFLVSNTCAMYCRHCTRKRKVGDKDSIPDREKILEGIAY
CCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
IRENTQIRDVLLSGGDPFMLSDESLDWILTELTAIPHVEVIRIGTRVPVVLPFRITNQLV
HHCCCCEEHHEECCCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHHH
DILKKHKPIWINTQFNHPKEMTPSAQAAVAKLVDAGIPLGNQSVLLARINDCPVIMKELV
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
HQLVKNRIRPYYLYQCDLSEGISHFRTPIAKGIEIMESLIGHTSGFAVPRYVVDAPGGGG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
KIPVSPNYLLTWSVNKVVLRNYEGIICTYQEPAVYDRIICDQNCDACKLQLNIRNADESK
CCCCCCCEEEEEECCHHEEECCCCEEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCHH
VVGVAKLLADYDDTTTLIPENNERLERREDETN
HHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCC
>Mature Secondary Structure 
TLQTESLEKTADTETQTNESGAFSKGRYAGIDDGEYWKNWKWQVRHAVRSIDMVQQVLG
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHC
ITFDPKEREELQRTVEKFPISITPYYLSLIDTEDYRNDPIFRQAFPSPAELIVENYELSD
CCCCCHHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCCHHHCCCCHHHHHEECCCCCC
PLAEDKDSPCECITHRYPDRVLFLVSNTCAMYCRHCTRKRKVGDKDSIPDREKILEGIAY
CCCCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
IRENTQIRDVLLSGGDPFMLSDESLDWILTELTAIPHVEVIRIGTRVPVVLPFRITNQLV
HHCCCCEEHHEECCCCCEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHHH
DILKKHKPIWINTQFNHPKEMTPSAQAAVAKLVDAGIPLGNQSVLLARINDCPVIMKELV
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
HQLVKNRIRPYYLYQCDLSEGISHFRTPIAKGIEIMESLIGHTSGFAVPRYVVDAPGGGG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
KIPVSPNYLLTWSVNKVVLRNYEGIICTYQEPAVYDRIICDQNCDACKLQLNIRNADESK
CCCCCCCEEEEEECCHHEEECCCCEEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCHH
VVGVAKLLADYDDTTTLIPENNERLERREDETN
HHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10629195 [H]