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The map label for this gene is hisJ [H]

Identifier: 124485037

GI number: 124485037

Start: 200952

End: 201743

Strand: Reverse

Name: hisJ [H]

Synonym: Mlab_0210

Alternate gene names: 124485037

Gene position: 201743-200952 (Counterclockwise)

Preceding gene: 124485038

Following gene: 124485035

Centisome position: 11.18

GC content: 46.72

Gene sequence:

>792_bases
ATGGATAGAAAAGTGTTTTTTGGGGTAATCATAGCCCTTCTTGTTGTATCTGCTGTTCTAACCGCAGGTTGTGTCAGTAA
CCAAACTGAGAAAAAAACGTATATCGTGGGTATCGACGGAGACTACCCGCCCTATTCGTACGTTGATGAAAGCGGCAACT
TCGTCGGATTCGATGTTGAATCGATCCAGTGGATCGCAGAACAGGAAGGATTCAATGTCGAAATCAAGGCAGTCGCATGG
GACGGAATCATTCCGGCACTTCTTGCAAACAAGATCGATATGGTCTACTCAGGAATGACTATCACACCTGAGCGTGCTGC
ACAGGTGACCTTCTCAAAGCCGTACTGGAGTGTGGACCAGGGTGTTGCCGTAAAGAACGGTTCTACCGTAACGCTGGATC
AGTTCAAGGCAGGAAATCTTACGATCGGTGTCCAGAGAAGCTGCTCTGCTGATCAGTATATCCAGAGTGATGACTTCTTC
GGTCAGGCAAAATATGACCAGATGGTAAAAGACGGAAAGATCAAACTCTACGACACCTTCCCGCAGTCGATGGTTGCTCT
GGAGAACGGACTCGTGCAGGCTGTCATCTTTGATGATGTAAACATCAAAGACTACATCAAAGGCAAAGATGGAATTGTCA
TGCTTGCTACAATCCCGACCGGCGAAGAGTATGCCGTAGCTATGAGAAACGGCGACACCGAGCTTCAGAAACTCATCAAC
GACGGACTCACCAAATTAATGGCTTCCGAGAAATGGAACGAACTTAAAGCAAAGTACATTATCGAAGCATAA

Upstream 100 bases:

>100_bases
ACGATATATGAATACAATTTCACACACTTCTTTTAATAGGTCACACCATTTTTTGAATGGGAAGTAAGTGATTTATAGGA
TAAATTAAAATGATTAACAG

Downstream 100 bases:

>100_bases
TTCGAGATCGGGTGCCAAAATCGGCAGCCTCTTATTTTTTAAAGATGTTGTGTCACTCCAAAATCTGAAGTGAATGAATC
AACTGAAAAATTAGTTTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDRKVFFGVIIALLVVSAVLTAGCVSNQTEKKTYIVGIDGDYPPYSYVDESGNFVGFDVESIQWIAEQEGFNVEIKAVAW
DGIIPALLANKIDMVYSGMTITPERAAQVTFSKPYWSVDQGVAVKNGSTVTLDQFKAGNLTIGVQRSCSADQYIQSDDFF
GQAKYDQMVKDGKIKLYDTFPQSMVALENGLVQAVIFDDVNIKDYIKGKDGIVMLATIPTGEEYAVAMRNGDTELQKLIN
DGLTKLMASEKWNELKAKYIIEA

Sequences:

>Translated_263_residues
MDRKVFFGVIIALLVVSAVLTAGCVSNQTEKKTYIVGIDGDYPPYSYVDESGNFVGFDVESIQWIAEQEGFNVEIKAVAW
DGIIPALLANKIDMVYSGMTITPERAAQVTFSKPYWSVDQGVAVKNGSTVTLDQFKAGNLTIGVQRSCSADQYIQSDDFF
GQAKYDQMVKDGKIKLYDTFPQSMVALENGLVQAVIFDDVNIKDYIKGKDGIVMLATIPTGEEYAVAMRNGDTELQKLIN
DGLTKLMASEKWNELKAKYIIEA
>Mature_263_residues
MDRKVFFGVIIALLVVSAVLTAGCVSNQTEKKTYIVGIDGDYPPYSYVDESGNFVGFDVESIQWIAEQEGFNVEIKAVAW
DGIIPALLANKIDMVYSGMTITPERAAQVTFSKPYWSVDQGVAVKNGSTVTLDQFKAGNLTIGVQRSCSADQYIQSDDFF
GQAKYDQMVKDGKIKLYDTFPQSMVALENGLVQAVIFDDVNIKDYIKGKDGIVMLATIPTGEEYAVAMRNGDTELQKLIN
DGLTKLMASEKWNELKAKYIIEA

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=267, Percent_Identity=26.9662921348315, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787088, Length=239, Percent_Identity=27.6150627615063, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=25.5060728744939, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=23.8866396761134, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788649, Length=263, Percent_Identity=25.4752851711027, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29011; Mature: 29011

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKVFFGVIIALLVVSAVLTAGCVSNQTEKKTYIVGIDGDYPPYSYVDESGNFVGFDVE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEHH
SIQWIAEQEGFNVEIKAVAWDGIIPALLANKIDMVYSGMTITPERAAQVTFSKPYWSVDQ
HHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCEECCCHHEEEEECCCCCCCCC
GVAVKNGSTVTLDQFKAGNLTIGVQRSCSADQYIQSDDFFGQAKYDQMVKDGKIKLYDTF
CEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECC
PQSMVALENGLVQAVIFDDVNIKDYIKGKDGIVMLATIPTGEEYAVAMRNGDTELQKLIN
CHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHH
DGLTKLMASEKWNELKAKYIIEA
HHHHHHHHHHHHHHCCEEEEECC
>Mature Secondary Structure
MDRKVFFGVIIALLVVSAVLTAGCVSNQTEKKTYIVGIDGDYPPYSYVDESGNFVGFDVE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEHH
SIQWIAEQEGFNVEIKAVAWDGIIPALLANKIDMVYSGMTITPERAAQVTFSKPYWSVDQ
HHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCEECCCHHEEEEECCCCCCCCC
GVAVKNGSTVTLDQFKAGNLTIGVQRSCSADQYIQSDDFFGQAKYDQMVKDGKIKLYDTF
CEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECC
PQSMVALENGLVQAVIFDDVNIKDYIKGKDGIVMLATIPTGEEYAVAMRNGDTELQKLIN
CHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHH
DGLTKLMASEKWNELKAKYIIEA
HHHHHHHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]