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The map label for this gene is cofH [H]

Identifier: 124484951

GI number: 124484951

Start: 111901

End: 112986

Strand: Reverse

Name: cofH [H]

Synonym: Mlab_0123

Alternate gene names: 124484951

Gene position: 112986-111901 (Counterclockwise)

Preceding gene: 124484952

Following gene: 124484948

Centisome position: 6.26

GC content: 51.1

Gene sequence:

>1086_bases
ATGAACGTCACCACCATTCTGAATGATGTCCTTGGAGGCTCACGGCTCACGGAAGATGAAGCTGCATTCCTGTTCTCGGT
GCAAAACCGGGATATATGGAAGGTTGCCGAGGCCGCCGATATCATTCGCGAACGAAAAAACGGCGATGTCGTAACATATG
TCCGAAACATGAACATCCACATGACCAACATCTGCAAAAACTGTTGCGGGTTATGTGCATTCGGACGAAAGAGTACCGAT
CCCGAGGCTTTCTGCTTCACGGATGAAGAGTTCCGGGAGCATACCAAAGATGCGGGCAGAAAAAAGGTCACCGAGGTCTC
GTATCTTTCCGGAATTCACCCGGAATTCACCATCGAAAGTTACGAAAAGATGATCCGGACATTCCACGAAGAGATCCCCG
GCATCCACGTTCACGGATGCAGCCCGGATGAGATTTTGTTTGCAGCGAACCAAAGCGACATCACAACAAAAGAAGCACTC
ATTCGGCTAAAAGATGCGGGACTCGGTTCAGTCCAGGGAACGGCGGCGGAGATTCTCGTCGACCGTGTTCGAAACATCAT
CTGCAGTAAAAAACTCTCCACGGCAGAGTGGGTCAGGATCATCAAAGAAGCCTCGGAAGTTGGATTTCGAGCAACGTCCA
CGATCATGTACGGATCGGTGGAAACGGAAAAGGAACGGGCACGCCATCTATCGGTCCTTCGCGACGTCCAGGACGAAACC
GGTGTATTTACCGAACTTGTTCCGCTGGCGTTCCTGCACAAAAATACGCCGCTCGAACGGGCAGGAATCGTAAACCATGA
TGCAACAGGACGCGAAGACATTCTGCTGATCGCCATTTCACGGCTGTTTTTGGACAACTTCGACAACATCCAGGTACCCT
GGTCGAAGATCGGACGAAAGGTCACACAGTTGTCCCTGATGGCGGGAGGAAATGATGTCGGGGGGACGATGTTTGTGGAC
GCCCTTTCAAAGGATGCCGGCGGCGGAGATGAATCGGATTACTTCAGTCCCGAAGACATGAAAATAATGTGTGACGATAT
CGGCAGGACACTTCGTCAGAGAGACACGTTCTATAATCTCATCTGA

Upstream 100 bases:

>100_bases
TTGAGTCTGCCCTGATTGTGGTACTCATCATCGTTATCATTGAACTCGTCAGCAGAAAGCTGAAGAAGAACAAAGAGACC
AAAACGAAAGGAAAGAAAAA

Downstream 100 bases:

>100_bases
ACATCCCTTTTTCTCTTTTTTCAGCTCATCTATTTTCTTACAGTAGAAGGATCCCAAGAACCAGATCCACAAGAGAAAAT
CCAAAGAGGGCAAAGATATA

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNVTTILNDVLGGSRLTEDEAAFLFSVQNRDIWKVAEAADIIRERKNGDVVTYVRNMNIHMTNICKNCCGLCAFGRKSTD
PEAFCFTDEEFREHTKDAGRKKVTEVSYLSGIHPEFTIESYEKMIRTFHEEIPGIHVHGCSPDEILFAANQSDITTKEAL
IRLKDAGLGSVQGTAAEILVDRVRNIICSKKLSTAEWVRIIKEASEVGFRATSTIMYGSVETEKERARHLSVLRDVQDET
GVFTELVPLAFLHKNTPLERAGIVNHDATGREDILLIAISRLFLDNFDNIQVPWSKIGRKVTQLSLMAGGNDVGGTMFVD
ALSKDAGGGDESDYFSPEDMKIMCDDIGRTLRQRDTFYNLI

Sequences:

>Translated_361_residues
MNVTTILNDVLGGSRLTEDEAAFLFSVQNRDIWKVAEAADIIRERKNGDVVTYVRNMNIHMTNICKNCCGLCAFGRKSTD
PEAFCFTDEEFREHTKDAGRKKVTEVSYLSGIHPEFTIESYEKMIRTFHEEIPGIHVHGCSPDEILFAANQSDITTKEAL
IRLKDAGLGSVQGTAAEILVDRVRNIICSKKLSTAEWVRIIKEASEVGFRATSTIMYGSVETEKERARHLSVLRDVQDET
GVFTELVPLAFLHKNTPLERAGIVNHDATGREDILLIAISRLFLDNFDNIQVPWSKIGRKVTQLSLMAGGNDVGGTMFVD
ALSKDAGGGDESDYFSPEDMKIMCDDIGRTLRQRDTFYNLI
>Mature_361_residues
MNVTTILNDVLGGSRLTEDEAAFLFSVQNRDIWKVAEAADIIRERKNGDVVTYVRNMNIHMTNICKNCCGLCAFGRKSTD
PEAFCFTDEEFREHTKDAGRKKVTEVSYLSGIHPEFTIESYEKMIRTFHEEIPGIHVHGCSPDEILFAANQSDITTKEAL
IRLKDAGLGSVQGTAAEILVDRVRNIICSKKLSTAEWVRIIKEASEVGFRATSTIMYGSVETEKERARHLSVLRDVQDET
GVFTELVPLAFLHKNTPLERAGIVNHDATGREDILLIAISRLFLDNFDNIQVPWSKIGRKVTQLSLMAGGNDVGGTMFVD
ALSKDAGGGDESDYFSPEDMKIMCDDIGRTLRQRDTFYNLI

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40366; Mature: 40366

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTTILNDVLGGSRLTEDEAAFLFSVQNRDIWKVAEAADIIRERKNGDVVTYVRNMNIH
CCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEE
MTNICKNCCGLCAFGRKSTDPEAFCFTDEEFREHTKDAGRKKVTEVSYLSGIHPEFTIES
HHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YEKMIRTFHEEIPGIHVHGCSPDEILFAANQSDITTKEALIRLKDAGLGSVQGTAAEILV
HHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
DRVRNIICSKKLSTAEWVRIIKEASEVGFRATSTIMYGSVETEKERARHLSVLRDVQDET
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GVFTELVPLAFLHKNTPLERAGIVNHDATGREDILLIAISRLFLDNFDNIQVPWSKIGRK
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHH
VTQLSLMAGGNDVGGTMFVDALSKDAGGGDESDYFSPEDMKIMCDDIGRTLRQRDTFYNL
HHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
I
C
>Mature Secondary Structure
MNVTTILNDVLGGSRLTEDEAAFLFSVQNRDIWKVAEAADIIRERKNGDVVTYVRNMNIH
CCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEE
MTNICKNCCGLCAFGRKSTDPEAFCFTDEEFREHTKDAGRKKVTEVSYLSGIHPEFTIES
HHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YEKMIRTFHEEIPGIHVHGCSPDEILFAANQSDITTKEALIRLKDAGLGSVQGTAAEILV
HHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
DRVRNIICSKKLSTAEWVRIIKEASEVGFRATSTIMYGSVETEKERARHLSVLRDVQDET
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GVFTELVPLAFLHKNTPLERAGIVNHDATGREDILLIAISRLFLDNFDNIQVPWSKIGRK
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHH
VTQLSLMAGGNDVGGTMFVDALSKDAGGGDESDYFSPEDMKIMCDDIGRTLRQRDTFYNL
HHHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA