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The map label for this gene is frhG [H]

Identifier: 124484842

GI number: 124484842

Start: 13004

End: 13915

Strand: Reverse

Name: frhG [H]

Synonym: Mlab_0013

Alternate gene names: 124484842

Gene position: 13915-13004 (Counterclockwise)

Preceding gene: 124484843

Following gene: 124484841

Centisome position: 0.77

GC content: 52.08

Gene sequence:

>912_bases
ATGGGATTATTAGATAAACTCAAAGGCTTACTGTTCGGTAATGAGGCTGTCAGCGAGGGAAAACAGGACGCAAAGCCTGG
TGCAACACCAGCTGACATAATGGTCGAACAAAAGCCAATTATTACAAAACCAAAGGAGGAGAAGCCAGTGGCTGACAAGA
TTACTGTAGGACACGTACACATGAGCGGTTGTACCGGATGTCTCGTGTCCCTTGCAGATAACTACGAAGGATTACTTACA
ATCCTCGACAAATACGCTGACCTCGTCTACTGTCTGACTCTCGCCGATGTGCGCCACATCCCAAAAATGGATGTCGCATT
AGTCGAGGGTTCCGTCTGTATCAACGACGAGTGCTCAATGGAAGAGATTTTAGAAACCCGTGAGAATGCAGCAATTGTTG
TAGCTCTCGGAGGCTGTGCCTGCTACGGAAACACTACCCGTTTCTGCCGCGGCGGACAGCAGAACCAACCCCAGCATGAG
GGTTTCCTGCCAATCGGCGACCTGATCAAGGTCGATGTGTACATCCCGGCATGTGCACCGACACCGCAGCAGATCAGAAA
CGTCTGTCTTATGGCATACCTGTTACTCAAAGGAAACGACGAGCAGAAGGCACTTGCAACCGCATACCTTACCCCTCTCA
TGAACCTTGCAGCAGCAGGAACCGAAGCATGCGGCTGCGACCTGATGACCGAAGTCATCAACCAGGGTCTCTGCTGCGGA
TGCGGCAGCTGCGCAGCAGCCTGCCCGGTCCGTGCAGTCACGATCCAGTACGGCAGACCGCAGATTGAGCGCGACCTCTG
TATCAAATGTGGAGCATGCTACGCACAGTGCCCGAGAAGCTTCTTCAGCTTCGACGTCTGTGGACAGTATGAAGCAATCA
CTAACCTCATTGGAGAGGTTATGAAACCCTGA

Upstream 100 bases:

>100_bases
TCCGGATTCAGAGGACTTTGCCCTCGAATTAAGTCCGGAAGTCCAAGGAGCTGTTCCCAAAACTGTAACTGTCATCTTAC
AGGAATTAGAGAGCTGAAAA

Downstream 100 bases:

>100_bases
GGGTCCTGGAGGCGAAAAAAATGGAATATCTTGGACAATACAAGGCAGTCTACAAAGCCAAAGCCGGCTGTGAAGACATT
CTCGCAAAGTCCCAGGACGG

Product: coenzyme F420-reducing hydrogenase, gamma subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma; FRH [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGLLDKLKGLLFGNEAVSEGKQDAKPGATPADIMVEQKPIITKPKEEKPVADKITVGHVHMSGCTGCLVSLADNYEGLLT
ILDKYADLVYCLTLADVRHIPKMDVALVEGSVCINDECSMEEILETRENAAIVVALGGCACYGNTTRFCRGGQQNQPQHE
GFLPIGDLIKVDVYIPACAPTPQQIRNVCLMAYLLLKGNDEQKALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCG
CGSCAAACPVRAVTIQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEVMKP

Sequences:

>Translated_303_residues
MGLLDKLKGLLFGNEAVSEGKQDAKPGATPADIMVEQKPIITKPKEEKPVADKITVGHVHMSGCTGCLVSLADNYEGLLT
ILDKYADLVYCLTLADVRHIPKMDVALVEGSVCINDECSMEEILETRENAAIVVALGGCACYGNTTRFCRGGQQNQPQHE
GFLPIGDLIKVDVYIPACAPTPQQIRNVCLMAYLLLKGNDEQKALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCG
CGSCAAACPVRAVTIQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEVMKP
>Mature_302_residues
GLLDKLKGLLFGNEAVSEGKQDAKPGATPADIMVEQKPIITKPKEEKPVADKITVGHVHMSGCTGCLVSLADNYEGLLTI
LDKYADLVYCLTLADVRHIPKMDVALVEGSVCINDECSMEEILETRENAAIVVALGGCACYGNTTRFCRGGQQNQPQHEG
FLPIGDLIKVDVYIPACAPTPQQIRNVCLMAYLLLKGNDEQKALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCGC
GSCAAACPVRAVTIQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEVMKP

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1941

COG function: function code C; Coenzyme F420-reducing hydrogenase, gamma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017681
- InterPro:   IPR006137
- InterPro:   IPR014406 [H]

Pfam domain/function: PF00037 Fer4; PF01058 Oxidored_q6 [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 32499; Mature: 32368

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
7.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLDKLKGLLFGNEAVSEGKQDAKPGATPADIMVEQKPIITKPKEEKPVADKITVGHVH
CCHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCEEEEEEE
MSGCTGCLVSLADNYEGLLTILDKYADLVYCLTLADVRHIPKMDVALVEGSVCINDECSM
ECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCH
EEILETRENAAIVVALGGCACYGNTTRFCRGGQQNQPQHEGFLPIGDLIKVDVYIPACAP
HHHHHHCCCCEEEEEECCEEEECCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
TPQQIRNVCLMAYLLLKGNDEQKALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
CGSCAAACPVRAVTIQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEV
CCCHHHHCCEEEEEEECCCCCCCHHHHHHCCHHHHHCCHHHHCHHCCCCHHHHHHHHHHH
MKP
HCC
>Mature Secondary Structure 
GLLDKLKGLLFGNEAVSEGKQDAKPGATPADIMVEQKPIITKPKEEKPVADKITVGHVH
CHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCEEEEEEE
MSGCTGCLVSLADNYEGLLTILDKYADLVYCLTLADVRHIPKMDVALVEGSVCINDECSM
ECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCH
EEILETRENAAIVVALGGCACYGNTTRFCRGGQQNQPQHEGFLPIGDLIKVDVYIPACAP
HHHHHHCCCCEEEEEECCEEEECCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
TPQQIRNVCLMAYLLLKGNDEQKALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
CGSCAAACPVRAVTIQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEV
CCCHHHHCCEEEEEEECCCCCCCHHHHHHCCHHHHHCCHHHHCHHCCCCHHHHHHHHHHH
MKP
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8521835 [H]