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The map label for this gene is vanB [H]

Identifier: 124266196

GI number: 124266196

Start: 1066404

End: 1067351

Strand: Direct

Name: vanB [H]

Synonym: Mpe_A1003

Alternate gene names: 124266196

Gene position: 1066404-1067351 (Clockwise)

Preceding gene: 124266194

Following gene: 124266197

Centisome position: 26.37

GC content: 72.68

Gene sequence:

>948_bases
TTGGAGGTGACGGTGGCCGGGCGCGAGACGGCGGCCGAGGGCGTGGCGAGCTTCGAGCTGCGGCGCGCCGACGGGGGGCC
GCTGCCGGGCTTCGACGCCGGGGCGCACATCGACGTGGAACTGCCGAACGGCATGGTGCGGCAGTACTCGCTGTGCAACG
ACCCGGCCGAGCGCGGTCACTACCGGATCGGCGTGCTGCTGGAGGCGGCGGGGCGCGGCGGATCGCGCAGCGCCCACGCG
CAGTTGCTGCCTGGCAGCCGGCTGCGCATCAGTGCGCCGCGCAACCATTTCGCGCTGGTCCCGGCGCGCCACACGCTGCT
GCTCGCGGGCGGCATCGGTGTCACGCCGATCCTGAGCATGGCCGAGCAGCTGGCGCGTGCCGATGCGTCGTTCGAGCTGC
ACTACTGCAGCCGCTCCCCGGCGCGCACCGCCTTCGTGCCGCGGCTGCGGGCCGCGGCGTTTGCCGCGCAGGTGCACTTC
CACTTCGACGACGGCGACGCCGCGCAGCGCTTCGATGCGCGCGCGGTGCTCGCGCAGGCGGGGGACACGACGCACCTCTA
CGTGTGCGGACCGAAGGGCTTCATGGACCACGTGATCCTGACCGCCCGCGCGCTGGGCTGGTCGGAGGGCAACATCCATT
ACGAGTACTTCGCGGGCGCCGCGGTCGACAGCTCGGCGGACCGCGGCTTCGAGGTGCAGATCGCCGGCAGCGGGCAGGTG
GTGCCGGTCGCGCCCGGCCAGAGCGTGGTGCAGGCGCTCGCCGCCCATGGCATCGATGTGCCGGTGTCGTGCGAGCAGGG
CGTCTGCGGCACCTGCGTGATGCGCGTCGTGCAGGGCGAGCCCGAGCACCGCGACATGTACTTCAGCGCGGCGGAGCACG
CCGCCAACCACTGCTTCACGCCGTGCTGCTCGCGCTCGAAGTCGGCGCGACTGGTGATCGATTTCTAG

Upstream 100 bases:

>100_bases
CCCGTGCTGGCATGGCACCGCGCTTGCAATTCTTCGTGTCCTGAACCGGCCGCAAGTGTCGGGCCGAGCATGGAGACCCG
CAGTGACGAACGATGGATGG

Downstream 100 bases:

>100_bases
TGTTCCGGCACCCCCCTCAGGAGACAGAAAGATGGAGCAGAAGATCGAATTGAAGCAGTCCTACCTGACCAACGCCTGGT
ACGTGGCGGCGCTGTCCACC

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MEVTVAGRETAAEGVASFELRRADGGPLPGFDAGAHIDVELPNGMVRQYSLCNDPAERGHYRIGVLLEAAGRGGSRSAHA
QLLPGSRLRISAPRNHFALVPARHTLLLAGGIGVTPILSMAEQLARADASFELHYCSRSPARTAFVPRLRAAAFAAQVHF
HFDDGDAAQRFDARAVLAQAGDTTHLYVCGPKGFMDHVILTARALGWSEGNIHYEYFAGAAVDSSADRGFEVQIAGSGQV
VPVAPGQSVVQALAAHGIDVPVSCEQGVCGTCVMRVVQGEPEHRDMYFSAAEHAANHCFTPCCSRSKSARLVIDF

Sequences:

>Translated_315_residues
MEVTVAGRETAAEGVASFELRRADGGPLPGFDAGAHIDVELPNGMVRQYSLCNDPAERGHYRIGVLLEAAGRGGSRSAHA
QLLPGSRLRISAPRNHFALVPARHTLLLAGGIGVTPILSMAEQLARADASFELHYCSRSPARTAFVPRLRAAAFAAQVHF
HFDDGDAAQRFDARAVLAQAGDTTHLYVCGPKGFMDHVILTARALGWSEGNIHYEYFAGAAVDSSADRGFEVQIAGSGQV
VPVAPGQSVVQALAAHGIDVPVSCEQGVCGTCVMRVVQGEPEHRDMYFSAAEHAANHCFTPCCSRSKSARLVIDF
>Mature_315_residues
MEVTVAGRETAAEGVASFELRRADGGPLPGFDAGAHIDVELPNGMVRQYSLCNDPAERGHYRIGVLLEAAGRGGSRSAHA
QLLPGSRLRISAPRNHFALVPARHTLLLAGGIGVTPILSMAEQLARADASFELHYCSRSPARTAFVPRLRAAAFAAQVHF
HFDDGDAAQRFDARAVLAQAGDTTHLYVCGPKGFMDHVILTARALGWSEGNIHYEYFAGAAVDSSADRGFEVQIAGSGQV
VPVAPGQSVVQALAAHGIDVPVSCEQGVCGTCVMRVVQGEPEHRDMYFSAAEHAANHCFTPCCSRSKSARLVIDF

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=38.125, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788903, Length=230, Percent_Identity=30, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787658, Length=322, Percent_Identity=25.1552795031056, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787098, Length=203, Percent_Identity=30.0492610837438, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 33472; Mature: 33472

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTVAGRETAAEGVASFELRRADGGPLPGFDAGAHIDVELPNGMVRQYSLCNDPAERGH
CEEEECCCHHHHHCHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCHHHCCC
YRIGVLLEAAGRGGSRSAHAQLLPGSRLRISAPRNHFALVPARHTLLLAGGIGVTPILSM
EEEEEEEEECCCCCCCCCCEEECCCCEEEEECCCCCEEEEECCEEEEEECCCCHHHHHHH
AEQLARADASFELHYCSRSPARTAFVPRLRAAAFAAQVHFHFDDGDAAQRFDARAVLAQA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHEECC
GDTTHLYVCGPKGFMDHVILTARALGWSEGNIHYEYFAGAAVDSSADRGFEVQIAGSGQV
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCEECCCCCCCEEEEECCCCEE
VPVAPGQSVVQALAAHGIDVPVSCEQGVCGTCVMRVVQGEPEHRDMYFSAAEHAANHCFT
EEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEEEHHHHHHHCCCC
PCCSRSKSARLVIDF
HHHCCCCCCEEEEEC
>Mature Secondary Structure
MEVTVAGRETAAEGVASFELRRADGGPLPGFDAGAHIDVELPNGMVRQYSLCNDPAERGH
CEEEECCCHHHHHCHHEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCHHHCCC
YRIGVLLEAAGRGGSRSAHAQLLPGSRLRISAPRNHFALVPARHTLLLAGGIGVTPILSM
EEEEEEEEECCCCCCCCCCEEECCCCEEEEECCCCCEEEEECCEEEEEECCCCHHHHHHH
AEQLARADASFELHYCSRSPARTAFVPRLRAAAFAAQVHFHFDDGDAAQRFDARAVLAQA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHEECC
GDTTHLYVCGPKGFMDHVILTARALGWSEGNIHYEYFAGAAVDSSADRGFEVQIAGSGQV
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCEECCCCCCCEEEEECCCCEE
VPVAPGQSVVQALAAHGIDVPVSCEQGVCGTCVMRVVQGEPEHRDMYFSAAEHAANHCFT
EEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEEEHHHHHHHCCCC
PCCSRSKSARLVIDF
HHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]