BacMap

Definition	Methylibium petroleiphilum PM1 chromosome, complete genome.
Accession	NC_008825
Length	4,044,195

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The map label for this gene is paaG [H]

Identifier: 124266177

GI number: 124266177

Start: 1046990

End: 1047790

Strand: Direct

Name: paaG [H]

Synonym: Mpe_A0984

Alternate gene names: 124266177

Gene position: 1046990-1047790 (Clockwise)

Preceding gene: 124266174

Following gene: 124266178

Centisome position: 25.89

GC content: 75.53

Gene sequence:

>801_bases
ATGTCCGACCCCACGGTCCTCGTCGCGCAGACCGGCAGCGTGCGGACGCTGACGCTGCACCGTCCCGCCGCGCTGAACAG
CTTCACCACCGAGATGCTCGGGCGCCTGCGCGGCGAGCTCGACGCGGCGGCCGGCGACGCCGGCGTGCGCTGCGTCGTGC
TGACCGGGGCCGGCCGCGCCTTCTGCGCCGGCCAGGACCTGGCCGACCCGGCCGTCGCGGTCGATCCCGCGCGCGGCGAG
CGCAAGGACCTGGGTGCCGTGATCGAGCGCTGGTACCGCCCGCTCGCGCTGCGCCTGCGCTCGATGCCGGTGCCGGTGGT
GGCCGCGGTCAACGGCGTCGCGGCCGGCGCCGGCGCCAACCTGGCGCTGAACTGCGACCTCGTGCTGGCGGCGCGTTCGG
CCGTGCTGATCCAGGCCTTCACGAAGATCGGCCTGGTGCCCGACACCGGCGGCACCTGGCTGCTGCCGCGACTGGCCGGC
CGCGCGAAGGCGCTCGGCCTGGCGCTGCTGGGCGACAAGCTGCCGGCCGCCGAGGCCGAGCGGCTCGGCCTGATCTGGCA
GTGCGTCGACGACGAAGCGCTGCGCGCCGAGGCCCAGGCGCTGGCCGACCGTCTGGCCGCGATGCCGACGCGCGCGCTGG
CGGCCACGCGCGAGGCGATCGACGCCGCCCAGACGCTCGACTTCGACACCGCGCTGCAGCGCGAACAGCAGCTGCAGCGC
GTGCAGGGTCATTCGAATGACTACCTCGAGGGCGTGAGCGCCTTCCTCGGCAAGCGCGCGCCGCGCTTCACCGACCGCTG
A

Upstream 100 bases:

>100_bases
GAACCGGAACATATAGTCTACCGACTGGTCGGTCAATCATGCATTGCCCGAGCGCACACGGCAGATGTCCCGGCATTCCC
TGGCAGAAAAGGCAGCACCG

Downstream 100 bases:

>100_bases
ACGCGCTGTTCCTCACCGCCCCCCGGACCCGGAGCCCTGCCATGACCGACGAACCCGCTTCCGCGCAGCGCCTCGCCGAG
CAGGTGCGCGACGGCCTGTG

Product: short chain enoyl-CoA hydratase / enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSDPTVLVAQTGSVRTLTLHRPAALNSFTTEMLGRLRGELDAAAGDAGVRCVVLTGAGRAFCAGQDLADPAVAVDPARGE
RKDLGAVIERWYRPLALRLRSMPVPVVAAVNGVAAGAGANLALNCDLVLAARSAVLIQAFTKIGLVPDTGGTWLLPRLAG
RAKALGLALLGDKLPAAEAERLGLIWQCVDDEALRAEAQALADRLAAMPTRALAATREAIDAAQTLDFDTALQREQQLQR
VQGHSNDYLEGVSAFLGKRAPRFTDR

Sequences:

>Translated_266_residues
MSDPTVLVAQTGSVRTLTLHRPAALNSFTTEMLGRLRGELDAAAGDAGVRCVVLTGAGRAFCAGQDLADPAVAVDPARGE
RKDLGAVIERWYRPLALRLRSMPVPVVAAVNGVAAGAGANLALNCDLVLAARSAVLIQAFTKIGLVPDTGGTWLLPRLAG
RAKALGLALLGDKLPAAEAERLGLIWQCVDDEALRAEAQALADRLAAMPTRALAATREAIDAAQTLDFDTALQREQQLQR
VQGHSNDYLEGVSAFLGKRAPRFTDR
>Mature_265_residues
SDPTVLVAQTGSVRTLTLHRPAALNSFTTEMLGRLRGELDAAAGDAGVRCVVLTGAGRAFCAGQDLADPAVAVDPARGER
KDLGAVIERWYRPLALRLRSMPVPVVAAVNGVAAGAGANLALNCDLVLAARSAVLIQAFTKIGLVPDTGGTWLLPRLAGR
AKALGLALLGDKLPAAEAERLGLIWQCVDDEALRAEAQALADRLAAMPTRALAATREAIDAAQTLDFDTALQREQQLQRV
QGHSNDYLEGVSAFLGKRAPRFTDR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=30.6513409961686, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI37594471, Length=239, Percent_Identity=28.4518828451883, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37594469, Length=239, Percent_Identity=28.4518828451883, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=25.78125, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=26.0700389105058, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=30.46875, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=24.8062015503876, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=24.8062015503876, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=24.8062015503876, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=24.8062015503876, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=25.0965250965251, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=25.0965250965251, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=25.4826254826255, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI68989263, Length=214, Percent_Identity=29.9065420560748, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=56.5217391304348, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=35.632183908046, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI221142681, Length=277, Percent_Identity=29.9638989169675, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=28.2442748091603, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082183, Length=222, Percent_Identity=24.7747747747748, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=27.4038461538462, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=262, Percent_Identity=34.3511450381679, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=28.957528957529, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=28.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=27.4436090225564, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=269, Percent_Identity=27.8810408921933, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=22.8682170542636, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=269, Percent_Identity=27.8810408921933, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=28.8065843621399, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=28.8135593220339, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=24.7104247104247, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=209, Percent_Identity=28.2296650717703, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=30.4511278195489, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=30.4511278195489, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=30.5882352941176, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=25.6704980842912, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24654903, Length=211, Percent_Identity=27.0142180094787, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920382, Length=268, Percent_Identity=28.3582089552239, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=234, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28571730, Length=184, Percent_Identity=28.804347826087, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571729, Length=184, Percent_Identity=28.804347826087, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28022; Mature: 27890

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPTVLVAQTGSVRTLTLHRPAALNSFTTEMLGRLRGELDAAAGDAGVRCVVLTGAGRA
CCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCE
FCAGQDLADPAVAVDPARGERKDLGAVIERWYRPLALRLRSMPVPVVAAVNGVAAGAGAN
EECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCC
LALNCDLVLAARSAVLIQAFTKIGLVPDTGGTWLLPRLAGRAKALGLALLGDKLPAAEAE
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCHHHHHHHHHHCCCCCCCHHH
RLGLIWQCVDDEALRAEAQALADRLAAMPTRALAATREAIDAAQTLDFDTALQREQQLQR
HHCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VQGHSNDYLEGVSAFLGKRAPRFTDR
HCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDPTVLVAQTGSVRTLTLHRPAALNSFTTEMLGRLRGELDAAAGDAGVRCVVLTGAGRA
CCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCE
FCAGQDLADPAVAVDPARGERKDLGAVIERWYRPLALRLRSMPVPVVAAVNGVAAGAGAN
EECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCC
LALNCDLVLAARSAVLIQAFTKIGLVPDTGGTWLLPRLAGRAKALGLALLGDKLPAAEAE
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCHHHHHHHHHHCCCCCCCHHH
RLGLIWQCVDDEALRAEAQALADRLAAMPTRALAATREAIDAAQTLDFDTALQREQQLQR
HHCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VQGHSNDYLEGVSAFLGKRAPRFTDR
HCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]