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The map label for this gene is oprM [H]

Identifier: 124266060

GI number: 124266060

Start: 902851

End: 904320

Strand: Reverse

Name: oprM [H]

Synonym: Mpe_A0867

Alternate gene names: 124266060

Gene position: 904320-902851 (Counterclockwise)

Preceding gene: 124266061

Following gene: 124266055

Centisome position: 22.36

GC content: 76.19

Gene sequence:

>1470_bases
TTGCCCTCCCTCGCCGGCCGCCTGCCGCGGCTGCCTCGGGCCGTGCTGGCCCCGCTGTTCGCAGCGCTGGTGCTGGCGGG
CTGCGCCACCGCCCCCGCCATCGACCCCGCGGCGCTGCCGGTCGCACCGGTGGCCTTCAAGGAAGGCGACGGCCGCTGGA
CCACGGCCGCCCCGGCTGACGCGCAGCCGCGCGGCACGTGGTGGAAGGCCTTCGCCGACCCCACGCTCGACGAGCTGGTG
GAGCGCGCCGGCCAGGCCAACAACAGCATCCAGACCGCTGCGGCGCGGCTCGCCCAGGCGCGCGCGCTGGTGCGCGCGGC
CGACGCCGACCGTGCGCCGCAGGTCGGTGTCGGTGCCGGCGTCACGCGCGGTACCGAGGCCGGCGCCGGCCCACGGCCGG
GCACGCTGATCAGCACTGGCGCCACCCTCGGCTACGAGGTCGACCTGTTCGGCCGCCTCGCTGGCGCCAGCCGTGCCGCC
GCGCTGGACGCGCGGTCGCGCGAGGCGCTGCTGCAGAGCACCCGCCTGCTGGTGCAGGCCGACGTGGCGCAGACCTACCT
CGCGCTGCGCGCCCTCGACGAGGAAAGCGCACTGGTGCGCGACACCGTCACCGCCTACCGCGACACGCTGAGCCTGATCG
AGCGTCGTTTCAACGCCGGCGACATCGCCGAGCTCGACGTGGCACGGGTGCGCACCCAGGTGGCGTCGACCGAGTCGGAG
GCGCTGGCGCTCGACCGCCGCCGCGCGGAACTGGAGCACGCGCTGGCAGTGCTGGTGGGCGCAGCGGCATCCGACTTCCA
CGTGGACATCGCGGACTGGCGCAGCGCGCTGCCGGTCATCCCGGCCGGCGTGCCGAGCACGCTGCTGGCACGGCGTCCCG
ACGTGTCGGCCGCGCAGCAGGCGATGCTGGCCGCGCAGGCGCGCGTCGGTGTCGCGAAGGCGGCCTGGTTCCCGAACCTC
TCGCTCACCGCCTCGGGCGGCGTCGCATCGCCGGAGCTGGGCGACCTGTTCCAGTGGTCGGCGCGCTCCTGGGGCATCGG
CGCGCTGCTGTCGCTGCCGCTGTTCGACGGCGGCCGGCGCGAGGCCGGCGTGCAGAACGCGAGCGCCGAGCTCGACGCTG
CGCTGGCCGGCTACCGCGAGCAGGTGCTGGTGGCGTTCCGCGATGTCGAGGACCAGCTGTCGGCGCTGCGCTACCTGGCC
GATCAGGACAGCGCGCAGGCCCGCGCGGTCGCGGCCGCGAGCCGTGCGACGGTGCTGTCGAGCTCGCGCTACCGCAACGG
CCTGGTGAGCCAGCTCGACCTGCTGGACGCGCAGCGCAGCGAACTCGCCAACCGCCGGCAGGCGCTGCAGGTGCGTTCGG
CGCAGTACCAGGCGACGGTGGGCCTGGTGCGCGCCCTGGGCGGCGCGTGGGACGCGCCGGCCGGGGTGGTGGGCACGGTC
GCGCCTGGGCAGGCCGCGGTCGGCAGCTGA

Upstream 100 bases:

>100_bases
AAGCCGCTGCCGGCCGCGCCGGTGGCCCACCACGACTGAAGCAGCGGACCGCTGCACTTCCTTCCGAGCAGAGAACGTCA
TGAAGCTTCCTTCTCCTCCT

Downstream 100 bases:

>100_bases
GCGGCGAGGCGGGTCGGTCGCGGCCTCAGCGCTCGCCGGGCTGGGGCGTGTGCAGGTTCCTGAGCCAGCGCATCCGCTCG
GCGTCGGGCGGTTTCGTCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MPSLAGRLPRLPRAVLAPLFAALVLAGCATAPAIDPAALPVAPVAFKEGDGRWTTAAPADAQPRGTWWKAFADPTLDELV
ERAGQANNSIQTAAARLAQARALVRAADADRAPQVGVGAGVTRGTEAGAGPRPGTLISTGATLGYEVDLFGRLAGASRAA
ALDARSREALLQSTRLLVQADVAQTYLALRALDEESALVRDTVTAYRDTLSLIERRFNAGDIAELDVARVRTQVASTESE
ALALDRRRAELEHALAVLVGAAASDFHVDIADWRSALPVIPAGVPSTLLARRPDVSAAQQAMLAAQARVGVAKAAWFPNL
SLTASGGVASPELGDLFQWSARSWGIGALLSLPLFDGGRREAGVQNASAELDAALAGYREQVLVAFRDVEDQLSALRYLA
DQDSAQARAVAAASRATVLSSSRYRNGLVSQLDLLDAQRSELANRRQALQVRSAQYQATVGLVRALGGAWDAPAGVVGTV
APGQAAVGS

Sequences:

>Translated_489_residues
MPSLAGRLPRLPRAVLAPLFAALVLAGCATAPAIDPAALPVAPVAFKEGDGRWTTAAPADAQPRGTWWKAFADPTLDELV
ERAGQANNSIQTAAARLAQARALVRAADADRAPQVGVGAGVTRGTEAGAGPRPGTLISTGATLGYEVDLFGRLAGASRAA
ALDARSREALLQSTRLLVQADVAQTYLALRALDEESALVRDTVTAYRDTLSLIERRFNAGDIAELDVARVRTQVASTESE
ALALDRRRAELEHALAVLVGAAASDFHVDIADWRSALPVIPAGVPSTLLARRPDVSAAQQAMLAAQARVGVAKAAWFPNL
SLTASGGVASPELGDLFQWSARSWGIGALLSLPLFDGGRREAGVQNASAELDAALAGYREQVLVAFRDVEDQLSALRYLA
DQDSAQARAVAAASRATVLSSSRYRNGLVSQLDLLDAQRSELANRRQALQVRSAQYQATVGLVRALGGAWDAPAGVVGTV
APGQAAVGS
>Mature_488_residues
PSLAGRLPRLPRAVLAPLFAALVLAGCATAPAIDPAALPVAPVAFKEGDGRWTTAAPADAQPRGTWWKAFADPTLDELVE
RAGQANNSIQTAAARLAQARALVRAADADRAPQVGVGAGVTRGTEAGAGPRPGTLISTGATLGYEVDLFGRLAGASRAAA
LDARSREALLQSTRLLVQADVAQTYLALRALDEESALVRDTVTAYRDTLSLIERRFNAGDIAELDVARVRTQVASTESEA
LALDRRRAELEHALAVLVGAAASDFHVDIADWRSALPVIPAGVPSTLLARRPDVSAAQQAMLAAQARVGVAKAAWFPNLS
LTASGGVASPELGDLFQWSARSWGIGALLSLPLFDGGRREAGVQNASAELDAALAGYREQVLVAFRDVEDQLSALRYLAD
QDSAQARAVAAASRATVLSSSRYRNGLVSQLDLLDAQRSELANRRQALQVRSAQYQATVGLVRALGGAWDAPAGVVGTVA
PGQAAVGS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=31.0869565217391, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI2367348, Length=484, Percent_Identity=26.6528925619835, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50918; Mature: 50787

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLAGRLPRLPRAVLAPLFAALVLAGCATAPAIDPAALPVAPVAFKEGDGRWTTAAPAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCC
AQPRGTWWKAFADPTLDELVERAGQANNSIQTAAARLAQARALVRAADADRAPQVGVGAG
CCCCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VTRGTEAGAGPRPGTLISTGATLGYEVDLFGRLAGASRAAALDARSREALLQSTRLLVQA
CCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DVAQTYLALRALDEESALVRDTVTAYRDTLSLIERRFNAGDIAELDVARVRTQVASTESE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
ALALDRRRAELEHALAVLVGAAASDFHVDIADWRSALPVIPAGVPSTLLARRPDVSAAQQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHH
AMLAAQARVGVAKAAWFPNLSLTASGGVASPELGDLFQWSARSWGIGALLSLPLFDGGRR
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCC
EAGVQNASAELDAALAGYREQVLVAFRDVEDQLSALRYLADQDSAQARAVAAASRATVLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SSRYRNGLVSQLDLLDAQRSELANRRQALQVRSAQYQATVGLVRALGGAWDAPAGVVGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
APGQAAVGS
CCCCCCCCC
>Mature Secondary Structure 
PSLAGRLPRLPRAVLAPLFAALVLAGCATAPAIDPAALPVAPVAFKEGDGRWTTAAPAD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCC
AQPRGTWWKAFADPTLDELVERAGQANNSIQTAAARLAQARALVRAADADRAPQVGVGAG
CCCCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VTRGTEAGAGPRPGTLISTGATLGYEVDLFGRLAGASRAAALDARSREALLQSTRLLVQA
CCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DVAQTYLALRALDEESALVRDTVTAYRDTLSLIERRFNAGDIAELDVARVRTQVASTESE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
ALALDRRRAELEHALAVLVGAAASDFHVDIADWRSALPVIPAGVPSTLLARRPDVSAAQQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHH
AMLAAQARVGVAKAAWFPNLSLTASGGVASPELGDLFQWSARSWGIGALLSLPLFDGGRR
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCC
EAGVQNASAELDAALAGYREQVLVAFRDVEDQLSALRYLADQDSAQARAVAAASRATVLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SSRYRNGLVSQLDLLDAQRSELANRRQALQVRSAQYQATVGLVRALGGAWDAPAGVVGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
APGQAAVGS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]