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The map label for this gene is guaD [H]

Identifier: 124265994

GI number: 124265994

Start: 838538

End: 839875

Strand: Direct

Name: guaD [H]

Synonym: Mpe_A0801

Alternate gene names: 124265994

Gene position: 838538-839875 (Clockwise)

Preceding gene: 124265993

Following gene: 124265995

Centisome position: 20.73

GC content: 72.12

Gene sequence:

>1338_bases
ATGAGCCAAAGGCTGGCCCTGTTCGGCGACCTGCTCGACATCGAGGTCGACCCCGGCTTCGCTGCCCCTGGCGACGCCGC
CGGCGTGCGCTACCGGCCCGACCACTGGCTGCTGGTCGAGGACGGCCGCATCGTCGGTGCCGAGCCGGCCCGCGCCGGCA
GCGGCCCCGACGCGAGCTGGCAGCGTGTGGACCACGCCGGGCGGCTGATCACGCCGGGCTTCATCGACACCCATGTGCAC
TGCCCGCAGCTCGACGTGATCGCGAGCTACGGCACCGCGTTGCTCGAGTGGCTGAACACCTACACCTTCCCGGCCGAGCT
GCGCTATGCCGATCCGCTGGTGGCGGCCAGCGGTGCCGAGCGCTTCGTCGATGCGCTGCTGGCGCACGGCACCACCTCGG
CGGTGGTGTTCCCGACTGTCCACAAGGGCGCGACCGAGGCGCTGTTCACCTCGGCCCGCGCACGCGGCATGCGGCTGGTG
GCCGGCAAGGTGCTGATGGACCGCCACGCGCCCGACGGCCTGCGCGACGACGTGCTGCAGGCCGAGCGAGATTGCGCCGA
TCTGATCGCGCGCTGGCACGGCAACGGCCGCCTGTCGTACGCGGTGACGGTGCGCTTCGCGGCCACCAGCACGCCGGAGC
AGCTGGCGATGGCCGGTCGGCTGTGCCGCGAACACCCGGGCGTGTACATGCAGACCCACGTGGCCGAGAACACCGACGAG
GTGCGCTGGATCGCCGAGCTGTTCCCCGAGGCTCGCAGCTACCTCGATGTCTACCACCGGCACGGTCTGCTGCACGAGCG
CGCGGTGCTGGCGCACGGCATCTGGCTCGACGACACCGACCGCGCGTTGCTGCGCGACACCGGTGCGCAGATCGCCTTCT
GCCCGTCGAGCAACCTGTTCCTCGGCAGTGGTTTGTTCGACTGGCAGGCCGCGGTCGACACCGGCTACCGCGTGTCGATG
GCCAGCGACGTGGGCGGCGGCACCAGCCTGTCGATGCTGCGCACGCTGGCCGATGCCTACAAGGTGCAGGCGCTGCGCGG
CGTGAAGCTCAGCGCCTGGAAGGCGCTGCATGCCGCGACGCGCGGCGCCGCCGAGGCGCTGGGCCTGGCGCACGAGATGG
GTCACCTCGGACATGGTGCGCTGGCTGACCTGGCGGTGTGGGACTGGGCGGTCGGCCCGGTCGCCACGCACCGCGATGCG
GTGGCGCGCCGCGGTCGTGCCGGCGTGTCGCCGCTGACTGCGCTGCACGAGCGCGTGTTCGCGTGGATGACGCTAGGCGA
CGAGCGCAATCTCGTCGCGACCTACGTGGCCGGCGCGTGCCGCCACGAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CTGCGCGGCGTGATGCGCGATGTGGCGCGCGGCCTGCCGAAGCCGGGTGCCGCGGCCCCGCGCGCCGGCGCTCACTGATG
ACCACGCAAGGGAGCACCGC

Downstream 100 bases:

>100_bases
GCGCGGAGGCGCGGGCTCGTAGAGCACGGGTCGCATCGGCGGGCGAATGGATTCGTTGTTGGATTCAACATGGTGCACGA
ATTGGTATACCAAAAGTGTG

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSQRLALFGDLLDIEVDPGFAAPGDAAGVRYRPDHWLLVEDGRIVGAEPARAGSGPDASWQRVDHAGRLITPGFIDTHVH
CPQLDVIASYGTALLEWLNTYTFPAELRYADPLVAASGAERFVDALLAHGTTSAVVFPTVHKGATEALFTSARARGMRLV
AGKVLMDRHAPDGLRDDVLQAERDCADLIARWHGNGRLSYAVTVRFAATSTPEQLAMAGRLCREHPGVYMQTHVAENTDE
VRWIAELFPEARSYLDVYHRHGLLHERAVLAHGIWLDDTDRALLRDTGAQIAFCPSSNLFLGSGLFDWQAAVDTGYRVSM
ASDVGGGTSLSMLRTLADAYKVQALRGVKLSAWKALHAATRGAAEALGLAHEMGHLGHGALADLAVWDWAVGPVATHRDA
VARRGRAGVSPLTALHERVFAWMTLGDERNLVATYVAGACRHERG

Sequences:

>Translated_445_residues
MSQRLALFGDLLDIEVDPGFAAPGDAAGVRYRPDHWLLVEDGRIVGAEPARAGSGPDASWQRVDHAGRLITPGFIDTHVH
CPQLDVIASYGTALLEWLNTYTFPAELRYADPLVAASGAERFVDALLAHGTTSAVVFPTVHKGATEALFTSARARGMRLV
AGKVLMDRHAPDGLRDDVLQAERDCADLIARWHGNGRLSYAVTVRFAATSTPEQLAMAGRLCREHPGVYMQTHVAENTDE
VRWIAELFPEARSYLDVYHRHGLLHERAVLAHGIWLDDTDRALLRDTGAQIAFCPSSNLFLGSGLFDWQAAVDTGYRVSM
ASDVGGGTSLSMLRTLADAYKVQALRGVKLSAWKALHAATRGAAEALGLAHEMGHLGHGALADLAVWDWAVGPVATHRDA
VARRGRAGVSPLTALHERVFAWMTLGDERNLVATYVAGACRHERG
>Mature_444_residues
SQRLALFGDLLDIEVDPGFAAPGDAAGVRYRPDHWLLVEDGRIVGAEPARAGSGPDASWQRVDHAGRLITPGFIDTHVHC
PQLDVIASYGTALLEWLNTYTFPAELRYADPLVAASGAERFVDALLAHGTTSAVVFPTVHKGATEALFTSARARGMRLVA
GKVLMDRHAPDGLRDDVLQAERDCADLIARWHGNGRLSYAVTVRFAATSTPEQLAMAGRLCREHPGVYMQTHVAENTDEV
RWIAELFPEARSYLDVYHRHGLLHERAVLAHGIWLDDTDRALLRDTGAQIAFCPSSNLFLGSGLFDWQAAVDTGYRVSMA
SDVGGGTSLSMLRTLADAYKVQALRGVKLSAWKALHAATRGAAEALGLAHEMGHLGHGALADLAVWDWAVGPVATHRDAV
ARRGRAGVSPLTALHERVFAWMTLGDERNLVATYVAGACRHERG

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=434, Percent_Identity=28.8018433179723, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1789249, Length=422, Percent_Identity=43.1279620853081, Blast_Score=353, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6319963, Length=391, Percent_Identity=29.923273657289, Blast_Score=182, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24643849, Length=397, Percent_Identity=32.9974811083123, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 48196; Mature: 48065

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRLALFGDLLDIEVDPGFAAPGDAAGVRYRPDHWLLVEDGRIVGAEPARAGSGPDASW
CCCCCEECCCEEEEEECCCCCCCCCCCCCEECCCEEEEEECCEEECCCCCCCCCCCCCHH
QRVDHAGRLITPGFIDTHVHCPQLDVIASYGTALLEWLNTYTFPAELRYADPLVAASGAE
HHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHH
RFVDALLAHGTTSAVVFPTVHKGATEALFTSARARGMRLVAGKVLMDRHAPDGLRDDVLQ
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCHHHHHHH
AERDCADLIARWHGNGRLSYAVTVRFAATSTPEQLAMAGRLCREHPGVYMQTHVAENTDE
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VRWIAELFPEARSYLDVYHRHGLLHERAVLAHGIWLDDTDRALLRDTGAQIAFCPSSNLF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCHHHHHCCCCEEEECCCCCEE
LGSGLFDWQAAVDTGYRVSMASDVGGGTSLSMLRTLADAYKVQALRGVKLSAWKALHAAT
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RGAAEALGLAHEMGHLGHGALADLAVWDWAVGPVATHRDAVARRGRAGVSPLTALHERVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
AWMTLGDERNLVATYVAGACRHERG
EEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQRLALFGDLLDIEVDPGFAAPGDAAGVRYRPDHWLLVEDGRIVGAEPARAGSGPDASW
CCCCEECCCEEEEEECCCCCCCCCCCCCEECCCEEEEEECCEEECCCCCCCCCCCCCHH
QRVDHAGRLITPGFIDTHVHCPQLDVIASYGTALLEWLNTYTFPAELRYADPLVAASGAE
HHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHH
RFVDALLAHGTTSAVVFPTVHKGATEALFTSARARGMRLVAGKVLMDRHAPDGLRDDVLQ
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCHHHHHHH
AERDCADLIARWHGNGRLSYAVTVRFAATSTPEQLAMAGRLCREHPGVYMQTHVAENTDE
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VRWIAELFPEARSYLDVYHRHGLLHERAVLAHGIWLDDTDRALLRDTGAQIAFCPSSNLF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCHHHHHCCCCEEEECCCCCEE
LGSGLFDWQAAVDTGYRVSMASDVGGGTSLSMLRTLADAYKVQALRGVKLSAWKALHAAT
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RGAAEALGLAHEMGHLGHGALADLAVWDWAVGPVATHRDAVARRGRAGVSPLTALHERVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
AWMTLGDERNLVATYVAGACRHERG
EEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]