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The map label for this gene is prfA

Identifier: 124265716

GI number: 124265716

Start: 560344

End: 561423

Strand: Reverse

Name: prfA

Synonym: Mpe_A0523

Alternate gene names: 124265716

Gene position: 561423-560344 (Counterclockwise)

Preceding gene: 124265717

Following gene: 124265715

Centisome position: 13.88

GC content: 70.83

Gene sequence:

>1080_bases
ATGAAACCCGCCCTGCGCCTGCAACTCGACCGCTACGTCCGCCGTCTCGCCGAGCTCGATTCGCTGCTCGCCGATCCCGC
GGTGATGCGGGACGCCGGCGGCTTCCGGGCTCTGAGCCGCGAACACGTCGAGGTGTCGGGCATCGTCGAGCGCCAGCGCC
GCCACGCCCAGCGTGAGCGCGACCTGGCGGCCGCCGCGGAAATGGCCTCGGACCCGGAGCTGGCCGCCATGGCCGCGGAG
GAAGCCGCGAGCGCGCAGGCCGAGCTCGATCGCCTGGCCACCGAGCTGCAGCGCCTGCTGTTGCCGCGAGACCCGGACGA
TGCGCGCTCGGTGTTCCTGGAGATCCGCGCCGGCACCGGCGGCGATGAATCGGCCCTGTTCGCCGGCGACCTGCTGCGGA
TGTACACCCGCTACGCCGAGCGCAAGGGCTGGCGCGTCGAGCTGCTGTCGGCGAGCGACAGCGAGCTGGGCGGCTACAAG
GAGGTCGTGGCGCGCATCGAGGGCGACGCGGTGTTCGGTCACCTGAAATACGAATCCGGCGGCCACCGCGTGCAGCGCGT
GCCCGCTACCGAGACGCAGGGCCGCATCCACACCAGCGCCTGCACCGTCGCCGTGCTGGCCGAGCCCGACGAAGCGCAGG
AGGTGACGCTGAATCCGGCCGACCTGCGCATCGACACCTACCGCGCCAGCGGGGCCGGCGGGCAGCACGTCAACAAGACC
GACTCGGCCGTGCGCATCACCCACCTTCCGACCGGGCTGGTGGCCGAATGCCAGGACGACCGCTCGCAACACCGCAACAA
GGCCAAGGCCATGGCGGTGCTCGCAGCCCGCCTGCGCGACAAGGACCGGCTGGAGCGGCAAGCCAGGGAAGCAGCCACAC
GCAAGAGCCTGATCGGCAGCGGCGACCGCAGTGACCGCATCCGCACCTACAACTTCCCGCAAGGGCGACTCACCGACCAC
CGCATCAACCTCACGCTCTACCAGCTCGGCGCAATCATGGACGGTGACCTCGACCCGGTCGTGAAGGCGCTGGTAGCGGC
GCGCGAGGCCGAGTTGCTGGCCGAGCTGGAAATCGGCTGA

Upstream 100 bases:

>100_bases
GCCCCGCGAAGACGATCATCTCGACGGGGTGTAGCCCGCCGGCCGTTGCCGGCTACCGGGAGCCGCAGCGGCTGCCGGTC
TGGCGCTCCCCTGCCCACCG

Downstream 100 bases:

>100_bases
GGATGAGCTGCCCAGTGCCGCCCCCCGACAGCGTCGCCGCCGCGCTGCTGGCGGCCCGCGAGCGCGGCATCGACCGGCTC
GACGCGCAGTGCCTGCTCAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRERDLAAAAEMASDPELAAMAAE
EAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTGGDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYK
EVVARIEGDAVFGHLKYESGGHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKT
DSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGSGDRSDRIRTYNFPQGRLTDH
RINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG

Sequences:

>Translated_359_residues
MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRERDLAAAAEMASDPELAAMAAE
EAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTGGDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYK
EVVARIEGDAVFGHLKYESGGHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKT
DSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGSGDRSDRIRTYNFPQGRLTDH
RINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG
>Mature_359_residues
MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRERDLAAAAEMASDPELAAMAAE
EAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTGGDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYK
EVVARIEGDAVFGHLKYESGGHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKT
DSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGSGDRSDRIRTYNFPQGRLTDH
RINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=47.6973684210526, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=40.1197604790419, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=47.7157360406091, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=58.4269662921348, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI2367172, Length=348, Percent_Identity=38.7931034482759, Blast_Score=221, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=241, Percent_Identity=39.4190871369295, Blast_Score=174, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=366, Percent_Identity=39.8907103825137, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=322, Percent_Identity=40.6832298136646, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_METPP (A2SD46)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019720.1
- ProteinModelPortal:   A2SD46
- SMR:   A2SD46
- STRING:   A2SD46
- GeneID:   4787346
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0523
- NMPDR:   fig|279263.3.peg.2767
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   A2SD46
- BioCyc:   MPET420662:MPE_A0523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39532; Mature: 39532

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRER
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
DLAAAAEMASDPELAAMAAEEAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCC
GDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYKEVVARIEGDAVFGHLKYESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKT
CCEEEECCCCCCCCEEEECCEEEEEEECCCCCCEEECCHHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGS
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRLTDHRINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG
CCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRER
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
DLAAAAEMASDPELAAMAAEEAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCC
GDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYKEVVARIEGDAVFGHLKYESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKT
CCEEEECCCCCCCCEEEECCEEEEEEECCCCCCEEECCHHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGS
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRLTDHRINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG
CCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA