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The map label for this gene is dusB [H]

Identifier: 124265679

GI number: 124265679

Start: 527809

End: 528858

Strand: Reverse

Name: dusB [H]

Synonym: Mpe_A0486

Alternate gene names: 124265679

Gene position: 528858-527809 (Counterclockwise)

Preceding gene: 124265681

Following gene: 124265678

Centisome position: 13.08

GC content: 70.48

Gene sequence:

>1050_bases
GTGTCTCCCCTGCGCATCGGCTCGATCGAGCTCCCGAACCGGCTGTTCGTGGCACCGATGGCGGGCGTCACCGACCGACC
GTTCCGCCAGCTGTGCAAGCGGCTCGGCGCCGGCTATGCGGTGAGCGAGATGGTCACCTCGCGCAAGGAACTCTGGCACA
GCCTGAAGACCTCGCGGCGTGCCAACCACGACGGTGAGGTCGCGCCGATCGCGGTGCAGATCGCCGGCACCGAGCCGCTG
ATGATGGCCGAGGCCGCCGCCTACAACATCGACCGCGGCGCGCAGGTCATCGACATCAACATGGGCTGCCCGGCCAAGAA
GGTCTGCAACGTGTGGGCCGGCTCGGCGCTGATGCGCGACGAGTCGCTCGCGCTGCGCATCGTCGAGGCGGTGGTCACCG
CCTGCGCGCCGCACGGCGTGCCGGTGACGCTGAAGATGCGCACCGGCTGGAACATGCAGAACCGCAATGCGGTGGCGCTG
GCGCGCGGCGCCGAGTCGCTCGGCATCGCGATGCTGACGGTGCACGGCCGCACGCGCGAGCAGGGCTACGCCGGCTCGGC
CGAACACGACACCGTGCGCGCGGTGAAGACCGCGGTGCAGGTGCCGGTGGTCGCCAACGGCGACATCGACTCGCCCGAGA
AGGCGCGCGACGTGCTCGCCGCAACCGGCGCCGACGCGGTGATGGTCGGCCGCGCTGCGCAGGGGCGGCCGTGGATCTTC
CGCGAGATCGCGCACTTCCTCGCCACCGGCGAGCGCCTGCCGCCGCCGCGTACCGCGCAGGCCAAGGCCTGGTTGATCGA
ACACCTGCACGACCACTACGGCCTGTACGGCGAATACGCCGGCGTGCGCACCGCGCGCAAGCACATCGGCTGGGCAGTGC
ACGCGTTGCCGGGCGGTGCCCTGTTCCGCGCCCGAATGAACACACTGGAGCGCTGCGAGGCGCAAGTCGCCGCTGTCGGC
GATTTCTTCGATGCCCTCGGGGTCGAGCATGAGCTGCTGCCGCAAGCAGCAGCGGCGCTCGAACCGTCCTTCGAACAAGA
GGCTGCATGA

Upstream 100 bases:

>100_bases
GATTGTCGATGCAGCGCAGCAAGCAGCGCCACCGATCGGTGCCCGACCTGCCCGGATCGCGCCGCTATACTCTGCCCTCT
TTTTAAGCACACCGCCTCCC

Downstream 100 bases:

>100_bases
CCAAGAAGCACATCGACGCGTGCATTCGCGAGGGCCTCGATGCCTACTTCAAGGACCTGCGCGGCGCCGATCCGCGCAAC
ATCTACGACATGGTGCTGCA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSPLRIGSIELPNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTSRKELWHSLKTSRRANHDGEVAPIAVQIAGTEPL
MMAEAAAYNIDRGAQVIDINMGCPAKKVCNVWAGSALMRDESLALRIVEAVVTACAPHGVPVTLKMRTGWNMQNRNAVAL
ARGAESLGIAMLTVHGRTREQGYAGSAEHDTVRAVKTAVQVPVVANGDIDSPEKARDVLAATGADAVMVGRAAQGRPWIF
REIAHFLATGERLPPPRTAQAKAWLIEHLHDHYGLYGEYAGVRTARKHIGWAVHALPGGALFRARMNTLERCEAQVAAVG
DFFDALGVEHELLPQAAAALEPSFEQEAA

Sequences:

>Translated_349_residues
MSPLRIGSIELPNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTSRKELWHSLKTSRRANHDGEVAPIAVQIAGTEPL
MMAEAAAYNIDRGAQVIDINMGCPAKKVCNVWAGSALMRDESLALRIVEAVVTACAPHGVPVTLKMRTGWNMQNRNAVAL
ARGAESLGIAMLTVHGRTREQGYAGSAEHDTVRAVKTAVQVPVVANGDIDSPEKARDVLAATGADAVMVGRAAQGRPWIF
REIAHFLATGERLPPPRTAQAKAWLIEHLHDHYGLYGEYAGVRTARKHIGWAVHALPGGALFRARMNTLERCEAQVAAVG
DFFDALGVEHELLPQAAAALEPSFEQEAA
>Mature_348_residues
SPLRIGSIELPNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTSRKELWHSLKTSRRANHDGEVAPIAVQIAGTEPLM
MAEAAAYNIDRGAQVIDINMGCPAKKVCNVWAGSALMRDESLALRIVEAVVTACAPHGVPVTLKMRTGWNMQNRNAVALA
RGAESLGIAMLTVHGRTREQGYAGSAEHDTVRAVKTAVQVPVVANGDIDSPEKARDVLAATGADAVMVGRAAQGRPWIFR
EIAHFLATGERLPPPRTAQAKAWLIEHLHDHYGLYGEYAGVRTARKHIGWAVHALPGGALFRARMNTLERCEAQVAAVGD
FFDALGVEHELLPQAAAALEPSFEQEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=324, Percent_Identity=29.9382716049383, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI239788462, Length=266, Percent_Identity=31.9548872180451, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI31742496, Length=297, Percent_Identity=26.5993265993266, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=30.4721030042918, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI8923374, Length=331, Percent_Identity=26.8882175226586, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=50.7788161993769, Blast_Score=316, Evalue=1e-87,
Organism=Escherichia coli, GI1788462, Length=253, Percent_Identity=31.6205533596838, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=319, Percent_Identity=28.5266457680251, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=27.6422764227642, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=31.1688311688312, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=266, Percent_Identity=27.4436090225564, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=26.7543859649123, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=295, Percent_Identity=32.2033898305085, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45549423, Length=256, Percent_Identity=30.46875, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=28.2608695652174, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37614; Mature: 37482

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLRIGSIELPNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTSRKELWHSLKTSRR
CCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
ANHDGEVAPIAVQIAGTEPLMMAEAAAYNIDRGAQVIDINMGCPAKKVCNVWAGSALMRD
CCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHCC
ESLALRIVEAVVTACAPHGVPVTLKMRTGWNMQNRNAVALARGAESLGIAMLTVHGRTRE
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCCCC
QGYAGSAEHDTVRAVKTAVQVPVVANGDIDSPEKARDVLAATGADAVMVGRAAQGRPWIF
CCCCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHH
REIAHFLATGERLPPPRTAQAKAWLIEHLHDHYGLYGEYAGVRTARKHIGWAVHALPGGA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCHH
LFRARMNTLERCEAQVAAVGDFFDALGVEHELLPQAAAALEPSFEQEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SPLRIGSIELPNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTSRKELWHSLKTSRR
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
ANHDGEVAPIAVQIAGTEPLMMAEAAAYNIDRGAQVIDINMGCPAKKVCNVWAGSALMRD
CCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHCC
ESLALRIVEAVVTACAPHGVPVTLKMRTGWNMQNRNAVALARGAESLGIAMLTVHGRTRE
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCCCC
QGYAGSAEHDTVRAVKTAVQVPVVANGDIDSPEKARDVLAATGADAVMVGRAAQGRPWIF
CCCCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHH
REIAHFLATGERLPPPRTAQAKAWLIEHLHDHYGLYGEYAGVRTARKHIGWAVHALPGGA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCHH
LFRARMNTLERCEAQVAAVGDFFDALGVEHELLPQAAAALEPSFEQEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]