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The map label for this gene is purH [H]

Identifier: 124265678

GI number: 124265678

Start: 525930

End: 527525

Strand: Reverse

Name: purH [H]

Synonym: Mpe_A0485

Alternate gene names: 124265678

Gene position: 527525-525930 (Counterclockwise)

Preceding gene: 124265679

Following gene: 124265677

Centisome position: 13.04

GC content: 69.86

Gene sequence:

>1596_bases
ATGGCCACCGCCCTCCTCTCCGTTTCCGACAAGACCGGCATCGTCGAACTCGCCCGGTCCCTGCATGCGCTGGGCGTGAA
GCTGCTCTCGACCGGTGGCACGGCCAGGCTGCTGGCCGACAGCGGCCTCCCGGTCACCGAGGTGGCCGACCACACCGGCT
TCCCCGAAATGCTCGACGGTCGCGTGAAGACGCTGCACCCGACCATCCACGGCGGCCTGCTGGCGCGCCGCGACCTGCCG
GCGCACATGGCCTCGCTGGCCGCGCACGGCATCGAGACGATCGACCTGCTGGTGGTCAACCTCTACCCCTTCGAGGCCAC
AGTCGCGAAGCCCGGCTGCACGCTGGAGGACGCGATCGAGAACATCGACATCGGCGGACCGGCGATGGTGCGTTCGGCCG
CCAAGAACTGGAAGGACGTGGCGGTGCTGACCGACGCCTCGCAGTACGCCGGCGTGCTGGCCGACCTGCAGCAGGACGGC
CGGGTGAGCGAGAGCACGCGCTTCGCGCTTGCGGTCGCGGCCTTCAACCGCATCAGCAACTACGACGCGGCCATCAGCGA
CCACCTGTCGGCGCTGCGCCCCGACGGCACGCGCGCCGAGTTCCCGGCGCAAAGCAACGGCCGCTTCGTCAAGCTGCAGG
ACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCCGCGTTCTACCGCGACCTGCACCCGGCGCCTGGCTCGCTGGTGAGC
GCCGTGCAGCTGCAGGGCAAGGAGCTGTCGTACAACAACATCGCCGACGCCGATGCGGCGTGGGAGTGCGTGAAAGGCTT
CGACGCTTCCGTCGACGGGCCGGCCTGCGTGATCGTCAAGCACGCCAACCCCTGCGGCGTGGCCCTCGGCGCCAACGCGG
CCGAGGCCTATGGCAAGGCCTTCCGCACCGACCCGACCTCCGCGTTCGGCGGCATCATCGCCTTCAACGTTCCGGTCGAC
GGCGCGGCAGCGCAGGCGATCGCGAAGCAGTTCGTCGAGGTGCTGATCGCCCCCGGCTATACCGACGAGGCGCGCGCCGT
GTTCGCCGCCAAGGCCAACACGCGCGTGCTGCAGATCTCGCTCGACGGCGTGCAGCGCGACGCGCCCGACGCCTGGTCGC
GCGGCCTCAATTCGCACGACATCAAGCGCGTCGGCTCGGGTCTGCTGATCCAGAGCGCCGACAACCACGTGCTCGGACTG
CAGGACCTGAAAGTCGTCACGAAGCTGGCGCCGACCGACGGACAGCTGGCCGACCTGCTGTTCGCGTGGAAGGTGGCCAA
GTTCGTCAAGAGCAATGCCATCGTGTTCTGCGGCGACGGCATGACGCTCGGCGTCGGCGCCGGCCAGATGAGCCGGCTCG
ACAGCGCGCGCATCGCCAGCATCAAGGCCAGCCACGCCGACCTGAGCCTGGCCGGCTCGGCGGTCGCGAGCGACGCCTTC
TTCCCGTTCCGCGACGGCCTCGACGTGCTGGCCGATGCCGGAGCGCGCAGCGTCATCCAGCCCGGCGGCAGCCTGCGCGA
CGACGAGGTGATCGCCGCCGCCAACGAACGCGGCATCGCGATGGTGCTGACAGGTGTGCGTCACTTCAGGCACTGA

Upstream 100 bases:

>100_bases
ACCGCAACACCCTGCGGCGCAAGTTGCTCGAGCACAAGCTCATCAAGCCCTGAGCGCGCCGTACTCCGTTTTCCGCCCCT
ACCCCGCTTCCACGACCGCC

Downstream 100 bases:

>100_bases
GCACGGCCGGCTGCGGTGCCCCGTGGCGCGTGCTCCGAATAGCAGGGGACCATGTCCGCCGCCGCCGACTCGGTAGGCCT
GTTGCTGTCCTTCCTGGCAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MATALLSVSDKTGIVELARSLHALGVKLLSTGGTARLLADSGLPVTEVADHTGFPEMLDGRVKTLHPTIHGGLLARRDLP
AHMASLAAHGIETIDLLVVNLYPFEATVAKPGCTLEDAIENIDIGGPAMVRSAAKNWKDVAVLTDASQYAGVLADLQQDG
RVSESTRFALAVAAFNRISNYDAAISDHLSALRPDGTRAEFPAQSNGRFVKLQDLRYGENPHQSAAFYRDLHPAPGSLVS
AVQLQGKELSYNNIADADAAWECVKGFDASVDGPACVIVKHANPCGVALGANAAEAYGKAFRTDPTSAFGGIIAFNVPVD
GAAAQAIAKQFVEVLIAPGYTDEARAVFAAKANTRVLQISLDGVQRDAPDAWSRGLNSHDIKRVGSGLLIQSADNHVLGL
QDLKVVTKLAPTDGQLADLLFAWKVAKFVKSNAIVFCGDGMTLGVGAGQMSRLDSARIASIKASHADLSLAGSAVASDAF
FPFRDGLDVLADAGARSVIQPGGSLRDDEVIAAANERGIAMVLTGVRHFRH

Sequences:

>Translated_531_residues
MATALLSVSDKTGIVELARSLHALGVKLLSTGGTARLLADSGLPVTEVADHTGFPEMLDGRVKTLHPTIHGGLLARRDLP
AHMASLAAHGIETIDLLVVNLYPFEATVAKPGCTLEDAIENIDIGGPAMVRSAAKNWKDVAVLTDASQYAGVLADLQQDG
RVSESTRFALAVAAFNRISNYDAAISDHLSALRPDGTRAEFPAQSNGRFVKLQDLRYGENPHQSAAFYRDLHPAPGSLVS
AVQLQGKELSYNNIADADAAWECVKGFDASVDGPACVIVKHANPCGVALGANAAEAYGKAFRTDPTSAFGGIIAFNVPVD
GAAAQAIAKQFVEVLIAPGYTDEARAVFAAKANTRVLQISLDGVQRDAPDAWSRGLNSHDIKRVGSGLLIQSADNHVLGL
QDLKVVTKLAPTDGQLADLLFAWKVAKFVKSNAIVFCGDGMTLGVGAGQMSRLDSARIASIKASHADLSLAGSAVASDAF
FPFRDGLDVLADAGARSVIQPGGSLRDDEVIAAANERGIAMVLTGVRHFRH
>Mature_530_residues
ATALLSVSDKTGIVELARSLHALGVKLLSTGGTARLLADSGLPVTEVADHTGFPEMLDGRVKTLHPTIHGGLLARRDLPA
HMASLAAHGIETIDLLVVNLYPFEATVAKPGCTLEDAIENIDIGGPAMVRSAAKNWKDVAVLTDASQYAGVLADLQQDGR
VSESTRFALAVAAFNRISNYDAAISDHLSALRPDGTRAEFPAQSNGRFVKLQDLRYGENPHQSAAFYRDLHPAPGSLVSA
VQLQGKELSYNNIADADAAWECVKGFDASVDGPACVIVKHANPCGVALGANAAEAYGKAFRTDPTSAFGGIIAFNVPVDG
AAAQAIAKQFVEVLIAPGYTDEARAVFAAKANTRVLQISLDGVQRDAPDAWSRGLNSHDIKRVGSGLLIQSADNHVLGLQ
DLKVVTKLAPTDGQLADLLFAWKVAKFVKSNAIVFCGDGMTLGVGAGQMSRLDSARIASIKASHADLSLAGSAVASDAFF
PFRDGLDVLADAGARSVIQPGGSLRDDEVIAAANERGIAMVLTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=487, Percent_Identity=35.9342915811088, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1790439, Length=536, Percent_Identity=56.9029850746269, Blast_Score=591, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI71985564, Length=487, Percent_Identity=34.0862422997947, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71985574, Length=319, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=489, Percent_Identity=36.40081799591, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6323768, Length=490, Percent_Identity=36.530612244898, Blast_Score=269, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24649832, Length=486, Percent_Identity=36.4197530864198, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55695; Mature: 55563

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATALLSVSDKTGIVELARSLHALGVKLLSTGGTARLLADSGLPVTEVADHTGFPEMLDG
CCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHCCCCHHHHCC
RVKTLHPTIHGGLLARRDLPAHMASLAAHGIETIDLLVVNLYPFEATVAKPGCTLEDAIE
CCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPAMVRSAAKNWKDVAVLTDASQYAGVLADLQQDGRVSESTRFALAVAAFNRISN
HCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
YDAAISDHLSALRPDGTRAEFPAQSNGRFVKLQDLRYGENPHQSAAFYRDLHPAPGSLVS
CHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHH
AVQLQGKELSYNNIADADAAWECVKGFDASVDGPACVIVKHANPCGVALGANAAEAYGKA
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
FRTDPTSAFGGIIAFNVPVDGAAAQAIAKQFVEVLIAPGYTDEARAVFAAKANTRVLQIS
HCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEE
LDGVQRDAPDAWSRGLNSHDIKRVGSGLLIQSADNHVLGLQDLKVVTKLAPTDGQLADLL
ECCCCCCCCHHHHCCCCCHHHHHHCCCEEEECCCCCEECHHHHHHHHHCCCCCCHHHHHH
FAWKVAKFVKSNAIVFCGDGMTLGVGAGQMSRLDSARIASIKASHADLSLAGSAVASDAF
HHHHHHHHHHCCEEEEECCCCEEEECCCHHHHCCCHHHHEEECCCCCCCCCCCHHHCCCC
FPFRDGLDVLADAGARSVIQPGGSLRDDEVIAAANERGIAMVLTGVRHFRH
CCCCCCHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure 
ATALLSVSDKTGIVELARSLHALGVKLLSTGGTARLLADSGLPVTEVADHTGFPEMLDG
CEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHCCCCHHHHCC
RVKTLHPTIHGGLLARRDLPAHMASLAAHGIETIDLLVVNLYPFEATVAKPGCTLEDAIE
CCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPAMVRSAAKNWKDVAVLTDASQYAGVLADLQQDGRVSESTRFALAVAAFNRISN
HCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
YDAAISDHLSALRPDGTRAEFPAQSNGRFVKLQDLRYGENPHQSAAFYRDLHPAPGSLVS
CHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHH
AVQLQGKELSYNNIADADAAWECVKGFDASVDGPACVIVKHANPCGVALGANAAEAYGKA
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHH
FRTDPTSAFGGIIAFNVPVDGAAAQAIAKQFVEVLIAPGYTDEARAVFAAKANTRVLQIS
HCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEE
LDGVQRDAPDAWSRGLNSHDIKRVGSGLLIQSADNHVLGLQDLKVVTKLAPTDGQLADLL
ECCCCCCCCHHHHCCCCCHHHHHHCCCEEEECCCCCEECHHHHHHHHHCCCCCCHHHHHH
FAWKVAKFVKSNAIVFCGDGMTLGVGAGQMSRLDSARIASIKASHADLSLAGSAVASDAF
HHHHHHHHHHCCEEEEECCCCEEEECCCHHHHCCCHHHHEEECCCCCCCCCCCHHHCCCC
FPFRDGLDVLADAGARSVIQPGGSLRDDEVIAAANERGIAMVLTGVRHFRH
CCCCCCHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA