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The map label for this gene is nuoF2 [H]

Identifier: 124265531

GI number: 124265531

Start: 370348

End: 372069

Strand: Reverse

Name: nuoF2 [H]

Synonym: Mpe_A0338

Alternate gene names: 124265531

Gene position: 372069-370348 (Counterclockwise)

Preceding gene: 124265533

Following gene: 124265530

Centisome position: 9.2

GC content: 70.5

Gene sequence:

>1722_bases
ATGAATGCGCCCGTGATCCCGATCCAGGTCCTGCGCGACCCGGCCGTCAAACCGCGTCGGGCCTCGGGCGACCGCCCGGT
CGACGCCCAGGCATTGAGCACGGTCCAGGCCCTGCTGGCGGCCAGGCCTCAGACCCCGTTGCGGCGCGACCTGCTGATCG
AGCACCTGCACACGCTGAACGATCATTTCGGTCAGCTGCGCACCGACCATCTGGCTGCTCTGGCTCAGTTACTGCGCCTT
TCCCAGGCCGAAGTTTATGAAGTGGCGAGCTTTTATCACCACTTCGAAGTGGTCCAGGCGAAGGCCGACGGCAGCTATCC
GGAGCCCGCGCCGCTGACCGTCCGCGTCTGCGACGGCATCGCCTGCGAGCTGACCGGCGCCCGGGATCTGCTGACCCGCC
TGCCCGCATTGCTGGGGACCGAGGTGCGCGTGATCGCCGCCCCGTGCATCGGCCGCTGCGAGCAGGCCCCGGCGGCCGTC
GTGCACCAGTGCCCGGTGCCGAACGCGACACCCGAAGCCGTGCAGGCTGCAGTGACCGCCGGCCGCACGAAGCACGAACC
CGCGCCTTACACCACGCTCCCGTCCTATCACGACGCCGGCGGCTACCGCACGCTGCGGCAGTGCCTGAGCGGCGAACGGC
CGCTGGAGACGGTGCTGTCGACGATGGAAGCTTCGGGTCTGCGCGGGCTCGGGGGGGCCGGCTTCCCGGCCGGGCGCAAG
TGGCGCATCGTGCGCGAGCAGGCGGCGCCGCGCCTGATGGCGGTGAACATCGACGAAGGCGAGCCGGGCACCTTCAAGGA
CCGCCATTACCTCGAGCGCGATCCGCACCGCTTCCTCGAGGGCCTGCTGATCGCGGCCTGGGCGGTCGGCGTCGACGCCG
TCTACATCTACCTGCGCGACGAGTACCACGGCTGCCGCACCCTGCTCGAGCGCGAGCTGGCGGCGCTGCGAGCCGACCCG
CCGATGCGCGACATGCCGGCGATCGAGCTGCGCCGCGGCGCCGGCGCCTACATCTGCGGCGAGGAGTCCGCGATGATCGA
GTCGATCGAAGGCAAGCGCGGCATGCCGCGCCTGAGACCGCCCTACGTGGCGCAGGTCGGCCTGTTCGGGCGCCCGACGC
TGGAGCACAACTTCGAGACGCTGTACTGGGTGCGCGACATCCTCGAGAAGGGTGGCGACTGGTTCGCCGGCCACGGCCGC
CACGAGCGCAAGGGGCTGCGCTCGTTCTCGGTGAGCGGCCGCGTGAAGAAGCCGGGCGTGCACCTCGCGCCGGCCGGCAT
CACGCTGCGCGAGCTGGTCGACGAGTACTGTGGCGGCATGCTGGACGGCCACCAGCTGCACGCCTACCTGCCGGGCGGCG
CCTCGGGCGGCATCCTGCCCGAGCGCCTGGCCGACGTGCCGCTGGACTTCGACACGCTGCAGCCGCACGGCTGCTTCATC
GGCTCGGCGGCGGTGGTGGTGTTGTCTGACCAGGACCGCGTGACGGACGTGGCGCGCAACCTGATGCGCTTCTTCGAGCA
CGAGAGCTGCGGGCAGTGCACGCCGTGCCGTTCGGGCACCGCCAAGGTCAACGAACTGATCGCCAGCGACCGCTGGGACC
TGCCGCTGCTCGCCGAGCTGTCGCAGGTGATGCGCGATGCGTCCATCTGCGGCCTGGGCCAGGCGGCGCCGAACCCGGTG
GACTGCGTGATCAAGTATTTTCCGGAAGAGCTGACGGCATGA

Upstream 100 bases:

>100_bases
GTGAGGGGGGCCCGCTATCGTCCCGGGACGAGACAGCGCCCGGGCATATGCTCATTGAAACATAAGCGAACGGCGCCGAT
ACCCAAGGAATTCCCGAGCT

Downstream 100 bases:

>100_bases
ACGCACCGCTGACTTCGAAGCTGCTCGCGCCACAGGCCGCGATGGTGGAGTTCTCGCTCGACGGCCGCACGGTCAGCGCC
GGCGCTGACGAGACGATCTG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MNAPVIPIQVLRDPAVKPRRASGDRPVDAQALSTVQALLAARPQTPLRRDLLIEHLHTLNDHFGQLRTDHLAALAQLLRL
SQAEVYEVASFYHHFEVVQAKADGSYPEPAPLTVRVCDGIACELTGARDLLTRLPALLGTEVRVIAAPCIGRCEQAPAAV
VHQCPVPNATPEAVQAAVTAGRTKHEPAPYTTLPSYHDAGGYRTLRQCLSGERPLETVLSTMEASGLRGLGGAGFPAGRK
WRIVREQAAPRLMAVNIDEGEPGTFKDRHYLERDPHRFLEGLLIAAWAVGVDAVYIYLRDEYHGCRTLLERELAALRADP
PMRDMPAIELRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNFETLYWVRDILEKGGDWFAGHGR
HERKGLRSFSVSGRVKKPGVHLAPAGITLRELVDEYCGGMLDGHQLHAYLPGGASGGILPERLADVPLDFDTLQPHGCFI
GSAAVVVLSDQDRVTDVARNLMRFFEHESCGQCTPCRSGTAKVNELIASDRWDLPLLAELSQVMRDASICGLGQAAPNPV
DCVIKYFPEELTA

Sequences:

>Translated_573_residues
MNAPVIPIQVLRDPAVKPRRASGDRPVDAQALSTVQALLAARPQTPLRRDLLIEHLHTLNDHFGQLRTDHLAALAQLLRL
SQAEVYEVASFYHHFEVVQAKADGSYPEPAPLTVRVCDGIACELTGARDLLTRLPALLGTEVRVIAAPCIGRCEQAPAAV
VHQCPVPNATPEAVQAAVTAGRTKHEPAPYTTLPSYHDAGGYRTLRQCLSGERPLETVLSTMEASGLRGLGGAGFPAGRK
WRIVREQAAPRLMAVNIDEGEPGTFKDRHYLERDPHRFLEGLLIAAWAVGVDAVYIYLRDEYHGCRTLLERELAALRADP
PMRDMPAIELRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNFETLYWVRDILEKGGDWFAGHGR
HERKGLRSFSVSGRVKKPGVHLAPAGITLRELVDEYCGGMLDGHQLHAYLPGGASGGILPERLADVPLDFDTLQPHGCFI
GSAAVVVLSDQDRVTDVARNLMRFFEHESCGQCTPCRSGTAKVNELIASDRWDLPLLAELSQVMRDASICGLGQAAPNPV
DCVIKYFPEELTA
>Mature_573_residues
MNAPVIPIQVLRDPAVKPRRASGDRPVDAQALSTVQALLAARPQTPLRRDLLIEHLHTLNDHFGQLRTDHLAALAQLLRL
SQAEVYEVASFYHHFEVVQAKADGSYPEPAPLTVRVCDGIACELTGARDLLTRLPALLGTEVRVIAAPCIGRCEQAPAAV
VHQCPVPNATPEAVQAAVTAGRTKHEPAPYTTLPSYHDAGGYRTLRQCLSGERPLETVLSTMEASGLRGLGGAGFPAGRK
WRIVREQAAPRLMAVNIDEGEPGTFKDRHYLERDPHRFLEGLLIAAWAVGVDAVYIYLRDEYHGCRTLLERELAALRADP
PMRDMPAIELRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNFETLYWVRDILEKGGDWFAGHGR
HERKGLRSFSVSGRVKKPGVHLAPAGITLRELVDEYCGGMLDGHQLHAYLPGGASGGILPERLADVPLDFDTLQPHGCFI
GSAAVVVLSDQDRVTDVARNLMRFFEHESCGQCTPCRSGTAKVNELIASDRWDLPLLAELSQVMRDASICGLGQAAPNPV
DCVIKYFPEELTA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=424, Percent_Identity=39.3867924528302, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI260656005, Length=424, Percent_Identity=39.3867924528302, Blast_Score=273, Evalue=4e-73,
Organism=Escherichia coli, GI1788620, Length=397, Percent_Identity=38.0352644836272, Blast_Score=249, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17531727, Length=430, Percent_Identity=37.906976744186, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI19920794, Length=387, Percent_Identity=39.2764857881137, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI45550452, Length=384, Percent_Identity=36.71875, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24653819, Length=376, Percent_Identity=32.7127659574468, Blast_Score=195, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=30.8781869688385, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 62689; Mature: 62689

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVIPIQVLRDPAVKPRRASGDRPVDAQALSTVQALLAARPQTPLRRDLLIEHLHTLN
CCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DHFGQLRTDHLAALAQLLRLSQAEVYEVASFYHHFEVVQAKADGSYPEPAPLTVRVCDGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCC
ACELTGARDLLTRLPALLGTEVRVIAAPCIGRCEQAPAAVVHQCPVPNATPEAVQAAVTA
EEEECCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHH
GRTKHEPAPYTTLPSYHDAGGYRTLRQCLSGERPLETVLSTMEASGLRGLGGAGFPAGRK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
WRIVREQAAPRLMAVNIDEGEPGTFKDRHYLERDPHRFLEGLLIAAWAVGVDAVYIYLRD
EEEEHHHCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEC
EYHGCRTLLERELAALRADPPMRDMPAIELRRGAGAYICGEESAMIESIEGKRGMPRLRP
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCC
PYVAQVGLFGRPTLEHNFETLYWVRDILEKGGDWFAGHGRHERKGLRSFSVSGRVKKPGV
CHHHHCCCCCCCCHHCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCCCCEECCCC
HLAPAGITLRELVDEYCGGMLDGHQLHAYLPGGASGGILPERLADVPLDFDTLQPHGCFI
EECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCEEE
GSAAVVVLSDQDRVTDVARNLMRFFEHESCGQCTPCRSGTAKVNELIASDRWDLPLLAEL
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
SQVMRDASICGLGQAAPNPVDCVIKYFPEELTA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAPVIPIQVLRDPAVKPRRASGDRPVDAQALSTVQALLAARPQTPLRRDLLIEHLHTLN
CCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DHFGQLRTDHLAALAQLLRLSQAEVYEVASFYHHFEVVQAKADGSYPEPAPLTVRVCDGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCC
ACELTGARDLLTRLPALLGTEVRVIAAPCIGRCEQAPAAVVHQCPVPNATPEAVQAAVTA
EEEECCHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHH
GRTKHEPAPYTTLPSYHDAGGYRTLRQCLSGERPLETVLSTMEASGLRGLGGAGFPAGRK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
WRIVREQAAPRLMAVNIDEGEPGTFKDRHYLERDPHRFLEGLLIAAWAVGVDAVYIYLRD
EEEEHHHCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEC
EYHGCRTLLERELAALRADPPMRDMPAIELRRGAGAYICGEESAMIESIEGKRGMPRLRP
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCC
PYVAQVGLFGRPTLEHNFETLYWVRDILEKGGDWFAGHGRHERKGLRSFSVSGRVKKPGV
CHHHHCCCCCCCCHHCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCCCCEECCCC
HLAPAGITLRELVDEYCGGMLDGHQLHAYLPGGASGGILPERLADVPLDFDTLQPHGCFI
EECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCEEE
GSAAVVVLSDQDRVTDVARNLMRFFEHESCGQCTPCRSGTAKVNELIASDRWDLPLLAEL
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
SQVMRDASICGLGQAAPNPVDCVIKYFPEELTA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]