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The map label for this gene is exoA [H]

Identifier: 124265282

GI number: 124265282

Start: 85229

End: 86020

Strand: Direct

Name: exoA [H]

Synonym: Mpe_A0089

Alternate gene names: 124265282

Gene position: 85229-86020 (Clockwise)

Preceding gene: 124265279

Following gene: 124265283

Centisome position: 2.11

GC content: 65.28

Gene sequence:

>792_bases
GTGTTGCGTCTGGTCTCGCTCAATCTCAACGGCATCCGTTCCGCGGCCAACAAGGGCTGGGTCGAATGGGCCGAGAAAAG
CGCCATCGATTGTATGGGCGTGCAGGAGCTCAAGGCCCAGGCGGCCGACCTGGGCGGGCGTTTCGACCAGGTGGCCGGCC
TGAAAGGCCACTTCCACTATGCCGACAAGAAGGGCTACTCCGGCGTCGGCCTGTACACGCGCGGCACGCCGAGCGACGTG
ATCACCGGCATCGGCGAGCCCGAGTTCGATGCCGAGGGGCGCTACGTCGAGGCCCGCTTCGACACGCCCCAGCGCAAGCT
GTCGATCATCAGTTGCTACTTTCCCAGCGGCTCCAGCTCCGACGAGCGGCAGCAGGCCAAGTTCCGTTTCCTGGCCCTGT
TCTACCCTTACCTCGAGCTGCTGAAGGCGACCCGCGAGTTCATCCTGGTGGGCGACGTGAACATCGCGCACCGCGAAATC
GACCTGAAGAACTGGAAGAGCAACCAGAAGAACAGCGGCTTCCTGCCCGAGGAGCGTGCGTGGATGAGCCGCGCGCTCGA
CGAACTGGGCCTGGTCGACGTGCACCGCACGCTGCGACCCGACGACACGGGCGAGGCTTACACGTGGTGGAGCAATCGAG
GCCAGGCCTGGGCCAACAACGTCGGTTGGCGCATCGACTACCACCTGGCGACGCCGGCGCTCGCCGCGCTGGCGCGTTCG
GCGTCGGTCTACAAGGCGCAGCGCTTTTCCGACCACGCCCCGTTGACGGTCGATTACGAACTTTCGCTGTGA

Upstream 100 bases:

>100_bases
GACCGCGAAGGCCACGAAGTCCTGTGCGAGGTCGTCTCGCGCCGCAGTGCCCGGGCCGCCGAATGTGCTCATCTGGACAT
CTGAAATAGGTAGGTGCGTC

Downstream 100 bases:

>100_bases
ACCTGCACGGCGTTTTCCCAGGAGATTTCTCATGCGCACCACGGCCCTGATCGTCGGCGTCTTGTTGCTTGTGGCGGGCG
GGCTGATCTCGGCCGGCGTC

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLRLVSLNLNGIRSAANKGWVEWAEKSAIDCMGVQELKAQAADLGGRFDQVAGLKGHFHYADKKGYSGVGLYTRGTPSDV
ITGIGEPEFDAEGRYVEARFDTPQRKLSIISCYFPSGSSSDERQQAKFRFLALFYPYLELLKATREFILVGDVNIAHREI
DLKNWKSNQKNSGFLPEERAWMSRALDELGLVDVHRTLRPDDTGEAYTWWSNRGQAWANNVGWRIDYHLATPALAALARS
ASVYKAQRFSDHAPLTVDYELSL

Sequences:

>Translated_263_residues
MLRLVSLNLNGIRSAANKGWVEWAEKSAIDCMGVQELKAQAADLGGRFDQVAGLKGHFHYADKKGYSGVGLYTRGTPSDV
ITGIGEPEFDAEGRYVEARFDTPQRKLSIISCYFPSGSSSDERQQAKFRFLALFYPYLELLKATREFILVGDVNIAHREI
DLKNWKSNQKNSGFLPEERAWMSRALDELGLVDVHRTLRPDDTGEAYTWWSNRGQAWANNVGWRIDYHLATPALAALARS
ASVYKAQRFSDHAPLTVDYELSL
>Mature_263_residues
MLRLVSLNLNGIRSAANKGWVEWAEKSAIDCMGVQELKAQAADLGGRFDQVAGLKGHFHYADKKGYSGVGLYTRGTPSDV
ITGIGEPEFDAEGRYVEARFDTPQRKLSIISCYFPSGSSSDERQQAKFRFLALFYPYLELLKATREFILVGDVNIAHREI
DLKNWKSNQKNSGFLPEERAWMSRALDELGLVDVHRTLRPDDTGEAYTWWSNRGQAWANNVGWRIDYHLATPALAALARS
ASVYKAQRFSDHAPLTVDYELSL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=35.6589147286822, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=35.6589147286822, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=35.6589147286822, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=27.1565495207668, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=31.8352059925094, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=198, Percent_Identity=34.3434343434343, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=34.1085271317829, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=34.1085271317829, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29598; Mature: 29598

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLVSLNLNGIRSAANKGWVEWAEKSAIDCMGVQELKAQAADLGGRFDQVAGLKGHFHY
CEEEEEECCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEE
ADKKGYSGVGLYTRGTPSDVITGIGEPEFDAEGRYVEARFDTPQRKLSIISCYFPSGSSS
CCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCC
DERQQAKFRFLALFYPYLELLKATREFILVGDVNIAHREIDLKNWKSNQKNSGFLPEERA
CHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEHHHCCCCCCCCCCCCHHHH
WMSRALDELGLVDVHRTLRPDDTGEAYTWWSNRGQAWANNVGWRIDYHLATPALAALARS
HHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCHHCCCCEEEEEEEHHHHHHHHHHH
ASVYKAQRFSDHAPLTVDYELSL
HHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure
MLRLVSLNLNGIRSAANKGWVEWAEKSAIDCMGVQELKAQAADLGGRFDQVAGLKGHFHY
CEEEEEECCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEE
ADKKGYSGVGLYTRGTPSDVITGIGEPEFDAEGRYVEARFDTPQRKLSIISCYFPSGSSS
CCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCC
DERQQAKFRFLALFYPYLELLKATREFILVGDVNIAHREIDLKNWKSNQKNSGFLPEERA
CHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEHHHCCCCCCCCCCCCHHHH
WMSRALDELGLVDVHRTLRPDDTGEAYTWWSNRGQAWANNVGWRIDYHLATPALAALARS
HHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCHHCCCCEEEEEEEHHHHHHHHHHH
ASVYKAQRFSDHAPLTVDYELSL
HHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]