Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 124024420

GI number: 124024420

Start: 2406175

End: 2407410

Strand: Reverse

Name: menE [H]

Synonym: P9303_27321

Alternate gene names: 124024420

Gene position: 2407410-2406175 (Counterclockwise)

Preceding gene: 124024421

Following gene: 124024419

Centisome position: 89.74

GC content: 57.61

Gene sequence:

>1236_bases
ATGAGGGGGTTGGTTGCCCTGCGTTGCAATCCCGCTCAGGCTGAGCAAAGGGCCTTGCAGCTTGCTCAGGCCTTGGAGCA
AGGCGATTGGGTGCAACTGCTGCCGCCCGTTGGAACTAGCGGTTTGATCCCTAGGGCTCTTTTGCCTGATGGTCCTGGTG
TGGTGGTCGCAAGTGGAGGCACCTCCGGCGGACAGCAGCACTGTTTGCAGCCTTGTAGCCATCTCGACTTATCAGCTTCG
GCCACAGGTCAATGGCTCCAGCAGCAGGGTTACGACCTGCAGCAATGCATGGTGCTTAATCCTTTGCCGTTGCATCACGT
TAGTGGCTTGATGTCTTGGTGGCGTAGTCGTTGTTGGCGAGTTGGGCATGCATGGCTACAGCCTGGCTTGATGAGGGATC
CAGACGCTTTAGAGCAGTTGTGTGGGTCGTTGCCGGGATGGGGAGAGCAACAGATCTTGATCTCTTTGGTGCCAACACAA
CTGCAGCGGTTGTTGACGCATAACGCTGGTGTGCGTGTGTTGAAAGCTTGTGCTGTGATTTGGGTTGGTGGAGCAGTGCT
GTCGCCAGTGCTGATGAAAGCGGCACGGGCCGAAGGGTTGCGCTTGGCACCCTGCTATGGAGCGACTGAGACGGCGGCGA
TGGTGACAGCGCTTGCTCCTGAGGATTTCCTGGCGGGTGCGACGGGCTGTGGATCACCGCTTGAAGATGTGGAGCTGCGG
TTGACGCAAGCAGGCGCTTTGCAAGTGCGAACAGCGCGGTTGGCGTTGGCGCGCTGGTTGGGTGAGGGCTTGGAGGATGT
TCGCGATCAGCAAGGCTGGTGGACCTCTGGAGATGCTGGTTGTTTGACAACTGAGAGCAACCAGCTTCGGCTCAATATCG
ATGGCCGCTTGGATGGGGCGATTCACTCTGGTGGTGAAACGGTGTTCCCTGAACAGTTGGAGTCCCGTTTACAAGCTGCG
GCGTTTGGTTTGCCTCTTGAGGCTGTGTTGCTGCTTGCCGTTGAAGACAGGGAATGGGGTCAACGCTTGGTGGCGCTTGT
GCGTCCTGCTGCTGGGGCGAATGGAGAGGATTTGATCAAGCACCTACGCCATCTCAGTGCCGATTGGCCTCCCGCTGATC
GGCCGATGCATTGGCGGCTTTGCCCAATGCTTGCTCCGATGGCGAATGGAAAATGGCAACGGGGCCATTGGCAGCAATGG
TTGCGGTTGCTAGAAGCTGAAAGTACAGAGTTTTGA

Upstream 100 bases:

>100_bases
AGCATCAGGGCCCTACACCAGTGGCTCCTGGCCTGGCCCCGGGTTGGTGCCCTGATGGACCTTTATTTAGCGCTGATCCC
GAAGTGGTCTGGGCGGCGGC

Downstream 100 bases:

>100_bases
CTATGGCAAGTCTTTCTGATACTGAGCTCAGCAATAAGAAATTAGCCGCGGGTTTGCTAGGGATCTTTCTTGGCGCTTTA
GGGATTCATAAATTCGTCTT

Product: O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRGLVALRCNPAQAEQRALQLAQALEQGDWVQLLPPVGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRVGHAWLQPGLMRDPDALEQLCGSLPGWGEQQILISLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPVLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELR
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLTTESNQLRLNIDGRLDGAIHSGGETVFPEQLESRLQAA
AFGLPLEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKHLRHLSADWPPADRPMHWRLCPMLAPMANGKWQRGHWQQW
LRLLEAESTEF

Sequences:

>Translated_411_residues
MRGLVALRCNPAQAEQRALQLAQALEQGDWVQLLPPVGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRVGHAWLQPGLMRDPDALEQLCGSLPGWGEQQILISLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPVLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELR
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLTTESNQLRLNIDGRLDGAIHSGGETVFPEQLESRLQAA
AFGLPLEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKHLRHLSADWPPADRPMHWRLCPMLAPMANGKWQRGHWQQW
LRLLEAESTEF
>Mature_411_residues
MRGLVALRCNPAQAEQRALQLAQALEQGDWVQLLPPVGTSGLIPRALLPDGPGVVVASGGTSGGQQHCLQPCSHLDLSAS
ATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWRVGHAWLQPGLMRDPDALEQLCGSLPGWGEQQILISLVPTQ
LQRLLTHNAGVRVLKACAVIWVGGAVLSPVLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELR
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLTTESNQLRLNIDGRLDGAIHSGGETVFPEQLESRLQAA
AFGLPLEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIKHLRHLSADWPPADRPMHWRLCPMLAPMANGKWQRGHWQQW
LRLLEAESTEF

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=249, Percent_Identity=34.136546184739, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=327, Percent_Identity=25.6880733944954, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 44639; Mature: 44639

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLVALRCNPAQAEQRALQLAQALEQGDWVQLLPPVGTSGLIPRALLPDGPGVVVASGG
CCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCEEEECCC
TSGGQQHCLQPCSHLDLSASATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VGHAWLQPGLMRDPDALEQLCGSLPGWGEQQILISLVPTQLQRLLTHNAGVRVLKACAVI
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WVGGAVLSPVLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELR
HHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHH
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLTTESNQLRLNIDGRLDGA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCC
IHSGGETVFPEQLESRLQAAAFGLPLEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIK
EECCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHCCCCCCCHHHHHH
HLRHLSADWPPADRPMHWRLCPMLAPMANGKWQRGHWQQWLRLLEAESTEF
HHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRGLVALRCNPAQAEQRALQLAQALEQGDWVQLLPPVGTSGLIPRALLPDGPGVVVASGG
CCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCEEEECCC
TSGGQQHCLQPCSHLDLSASATGQWLQQQGYDLQQCMVLNPLPLHHVSGLMSWWRSRCWR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VGHAWLQPGLMRDPDALEQLCGSLPGWGEQQILISLVPTQLQRLLTHNAGVRVLKACAVI
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WVGGAVLSPVLMKAARAEGLRLAPCYGATETAAMVTALAPEDFLAGATGCGSPLEDVELR
HHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHH
LTQAGALQVRTARLALARWLGEGLEDVRDQQGWWTSGDAGCLTTESNQLRLNIDGRLDGA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCC
IHSGGETVFPEQLESRLQAAAFGLPLEAVLLLAVEDREWGQRLVALVRPAAGANGEDLIK
EECCCCCCCHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHCCCCCCCHHHHHH
HLRHLSADWPPADRPMHWRLCPMLAPMANGKWQRGHWQQWLRLLEAESTEF
HHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]