Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 124024374

GI number: 124024374

Start: 2371325

End: 2372272

Strand: Reverse

Name: ppnK [H]

Synonym: P9303_26861

Alternate gene names: 124024374

Gene position: 2372272-2371325 (Counterclockwise)

Preceding gene: 124024375

Following gene: 124024373

Centisome position: 88.43

GC content: 55.17

Gene sequence:

>948_bases
ATGCGTCTCGATTTGGTCTGGGTGATCTATCGGGCGGGAAGTCATTCTGCGCATCGTGAAGCCTGTTGTTGTGTTGACGA
GCTCCAAGCGCTTGGAGTGAAAGTGGTGATGGCCATGAGTGGAGTGACGGCCAACCCTTTCCCCGATTTATTGGCCTCAG
AAGCCGGCCTGCCTGATTTGGCTGTTGTGCTTGGGGGCGATGGCACGGTGCTCGGTGCGGCCCGTCATCTTGCCGTTCAT
GATGTGCCAATCCTTAGTTTCAACGTCGGAGGGCATCTAGGTTTTCTGACGCATGAACGCTGTCTGCTTGATGGCGGTCA
GCTATGGCAGCGATTATTGCAGGACAACTTTGCTTTGGAGCGCAGGATGATGTTGCAGGCTGCAGTGGACAGCCGCTCCC
CTGCTGAACGAACTGCTCGACCTACTGCTTCGCTCCAGGATTTGAATGGGACTAAGCCACCGCACTGGGCCCTGAATGAT
TTTTATATGCGTCCTTATCGCGATGATGTGTCGCCTACCTGCACCCTTGAACTTGAGATCGATGGTGAGGTGGTGGATCA
TTACCGTGGCGATGGTTTGATCTTGGCGACCCCCACAGGGTCTACCGGCTACTCGATGGCGTCCGGAGGACCGATTCTTC
ACCCTGGCATTGATGCCATCATCGTGAGTCCAATTTGTCCGATGAGTCTCTCTAGTCGTCCCGTCATCGTGCCTCCAGCT
TCACGGCTCGTGATTGGGCTTCTGGGAGAAAACACTCGCCGGGTGAAGCTCTGGAAAGATGGGGCGAGTGGTGCCCTGCT
TGAACCAGGCCAGTGTTGTGTTGTTCAGAGGGCTCGGCACCATGCATTGATGGTGGTTCTTGAACAGAGCCCCTCTTATT
ACAGAACTCTCACCCACAAGCTGCATTGGGCGGGAAGTCTGCTCAACAATCAACCATCTCCCAGCTGA

Upstream 100 bases:

>100_bases
GGCAGCTGTGGGTCTTGCGATCCTCCTTTCCTTGTATCGCAATCGCGTCACTGTGGATATGGAGCGCTTCAATCTGCTGC
GCTGGTAAGGAGACCCAAGC

Downstream 100 bases:

>100_bases
AGCTCATGGGCCTCGAGATCGAACGGAGGTTTTTGGTGGTGGGAGAAGAGTGGCGATCTTTGGCAGGACCGGCCTTGCCT
TTGCGGCAGGGTTACTTGGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2 [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKPPHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS

Sequences:

>Translated_315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKPPHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS
>Mature_315_residues
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDLAVVLGGDGTVLGAARHLAVH
DVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALERRMMLQAAVDSRSPAERTARPTASLQDLNGTKPPHWALND
FYMRPYRDDVSPTCTLELEIDGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHKLHWAGSLLNNQPSPS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=256, Percent_Identity=29.296875, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788968, Length=261, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320794, Length=263, Percent_Identity=30.0380228136882, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322509, Length=263, Percent_Identity=30.0380228136882, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325068, Length=225, Percent_Identity=34.2222222222222, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28573828, Length=249, Percent_Identity=32.9317269076305, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28573830, Length=249, Percent_Identity=32.9317269076305, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573832, Length=254, Percent_Identity=32.6771653543307, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573826, Length=249, Percent_Identity=32.9317269076305, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161077047, Length=253, Percent_Identity=32.8063241106719, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129957, Length=279, Percent_Identity=28.673835125448, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653424, Length=279, Percent_Identity=28.673835125448, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363321, Length=279, Percent_Identity=28.673835125448, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363323, Length=279, Percent_Identity=28.673835125448, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653422, Length=279, Percent_Identity=28.673835125448, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 34070; Mature: 34070

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCE
AVVLGGDGTVLGAARHLAVHDVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALE
EEEECCCCEEEHHHHEEEEECCEEEEEECCCEEEEEECCHHHCCCHHHHHHHHHCCHHHH
RRMMLQAAVDSRSPAERTARPTASLQDLNGTKPPHWALNDFYMRPYRDDVSPTCTLELEI
HHHHHHHHHCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEE
DGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCH
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHK
HHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHH
LHWAGSLLNNQPSPS
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLDLVWVIYRAGSHSAHREACCCVDELQALGVKVVMAMSGVTANPFPDLLASEAGLPDL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCCE
AVVLGGDGTVLGAARHLAVHDVPILSFNVGGHLGFLTHERCLLDGGQLWQRLLQDNFALE
EEEECCCCEEEHHHHEEEEECCEEEEEECCCEEEEEECCHHHCCCHHHHHHHHHCCHHHH
RRMMLQAAVDSRSPAERTARPTASLQDLNGTKPPHWALNDFYMRPYRDDVSPTCTLELEI
HHHHHHHHHCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEE
DGEVVDHYRGDGLILATPTGSTGYSMASGGPILHPGIDAIIVSPICPMSLSSRPVIVPPA
CCCEEEEECCCEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCH
SRLVIGLLGENTRRVKLWKDGASGALLEPGQCCVVQRARHHALMVVLEQSPSYYRTLTHK
HHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHH
LHWAGSLLNNQPSPS
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]