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The map label for this gene is sspA [C]

Identifier: 124024300

GI number: 124024300

Start: 2306162

End: 2306887

Strand: Reverse

Name: sspA [C]

Synonym: P9303_26121

Alternate gene names: 124024300

Gene position: 2306887-2306162 (Counterclockwise)

Preceding gene: 124024307

Following gene: 124024299

Centisome position: 85.99

GC content: 54.68

Gene sequence:

>726_bases
ATGATGGAACTGCATCAATTCCGCCATTCCGCCTTCTGTCTCAAGGTGCGGATGGTCTTGCAGGCCAAGGGTTTGACTTA
CCAGGTGGTGGAAGTTACCCCCGGGTTAGGACAGCTCAACGTGTTTCGCTTGTCAGGTCAGCGTCAGGTCCCGGTACTTG
TCGATGGCGATGTTGTTTTGGCAGATTCCAGCACCATTGCTCGACATCTCGAGGCTAAGCAGCCGGAACCACCCCTCATT
CCGGTTGATCCACAGCAGGCCGCGCAGGTGTACTTGATTGAAGATTGGGCTGATACGACATTGGCGCGGGCAGGCCGGTC
AGCTTTGTTGCAGGCTGCAGCCTTGGACTCTGAGTTGCGTTTAGCTCTTCTTCCAGAAGATCTGCCAACTCCCTTGCGTC
AGGTCATGGGAGATCTCCCGGGGGGGTTGTTGAACGGTGCCTCTGAGATCATTGGCCAGGCAGAGAGGGTTGAGTTATTA
AAGAGCCTCGAACAATTGGCTGATTTGATGCAGAAAAGGTCCTGGCTAGTAGGAGATGCGATGAGCTTGGCTGATTTAGC
TGTAGCCGCTCAGATTTCTTTAATGCGTTTTCCAGCCTCGGCTGGGGCAGCACTTGCCGGTAGAGGTGTGTTCGGCCTGA
GTGATCACCCGCGGCTGCAGCCCTTATTCCACTGGAGGGATCAGCTTGAGTTGTCATTGCTTGAGACGGATGACTCAGCC
CGATGA

Upstream 100 bases:

>100_bases
TTAACTCTCAGCCGAAAGTGAAATGCGATTATTGCCCCCTTTGCCTTAGCGGGCTTTTGAAGCTGTTAGCTACTCTGCCG
TTCTTTTGGCAGAGCAAACG

Downstream 100 bases:

>100_bases
GCCTTGCTTGAACACCGATCCAGAAATGGCTGTCTAATGTCTGATCCCTTGATTCGTGATTCTGATCACTACGTCGTTTT
GGAGCCTGGCCAGCCTGAGC

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: Glutathione S-Transferase Domain-Containing Protein; Glutathione S-Transferase Domain Protein; Glutathione S-Transferase N-Terminal Domain Protein; Glutathione S-Transferase Zeta Class; Glutathione S-Transferase-Like

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGQAERVELL
KSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGRGVFGLSDHPRLQPLFHWRDQLELSLLETDDSA
R

Sequences:

>Translated_241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGQAERVELL
KSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGRGVFGLSDHPRLQPLFHWRDQLELSLLETDDSA
R
>Mature_241_residues
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVLADSSTIARHLEAKQPEPPLI
PVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELRLALLPEDLPTPLRQVMGDLPGGLLNGASEIIGQAERVELL
KSLEQLADLMQKRSWLVGDAMSLADLAVAAQISLMRFPASAGAALAGRGVFGLSDHPRLQPLFHWRDQLELSLLETDDSA
R

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.18

Molecular weight: Translated: 26252; Mature: 26252

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCEEE
ADSSTIARHLEAKQPEPPLIPVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELR
ECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
LALLPEDLPTPLRQVMGDLPGGLLNGASEIIGQAERVELLKSLEQLADLMQKRSWLVGDA
EEECCCCCCHHHHHHHHHCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCH
MSLADLAVAAQISLMRFPASAGAALAGRGVFGLSDHPRLQPLFHWRDQLELSLLETDDSA
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCEECCCCCCCCCHHHHHHCHHEEEEEECCCCC
R
C
>Mature Secondary Structure
MMELHQFRHSAFCLKVRMVLQAKGLTYQVVEVTPGLGQLNVFRLSGQRQVPVLVDGDVVL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCEEE
ADSSTIARHLEAKQPEPPLIPVDPQQAAQVYLIEDWADTTLARAGRSALLQAAALDSELR
ECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
LALLPEDLPTPLRQVMGDLPGGLLNGASEIIGQAERVELLKSLEQLADLMQKRSWLVGDA
EEECCCCCCHHHHHHHHHCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCH
MSLADLAVAAQISLMRFPASAGAALAGRGVFGLSDHPRLQPLFHWRDQLELSLLETDDSA
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCEECCCCCCCCCHHHHHHCHHEEEEEECCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA