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The map label for this gene is purH

Identifier: 124024174

GI number: 124024174

Start: 2182585

End: 2184138

Strand: Reverse

Name: purH

Synonym: P9303_24851

Alternate gene names: 124024174

Gene position: 2184138-2182585 (Counterclockwise)

Preceding gene: 124024176

Following gene: 124024170

Centisome position: 81.42

GC content: 57.21

Gene sequence:

>1554_bases
ATGGCTCCCATCGCCTTGTTGAGCGTTTCAAACAAACATGGGATTGTGTCTCTAGCGGAATCCTTGCATCGCATGCATGG
GTTTCAGCTGCTTTCCAGTGGTGGCACAGCCAAAGTGCTGGAGGATGCCGGTCTGCCCGTGACGCGGGTTGCTGAGCACA
CAGGAGCTGCGGAAATTCTTGGAGGACGGGTGAAGACTCTGCACCCTCGTGTCCATGGAGGCATCTTGGCGATGCGGGGC
GACCCTGATCATGAGGTCGATCTGGAGCAGCATCAGATCCCTCCTATTGATGTGGTGGTGGTGAACCTTTACCCGTTTCG
GGAAACAGTGGCCAATCCGCAGGTGAGTTGGGAAACCGCAATTGAGAATATTGATATCGGTGGCCCCGCCATGGTGCGAG
CGGCGGCAAAGAACCATGCGCATGTGGCCGTGCTCACGAGGCCGGATCAGTACGACAGATTCCTAGTGGCTTTAAGTGAT
GGTGTGGATGGCCAGCTCCGTCGTGAGCTGGCCCTTGAAGCCTTTGAGCACACCGCTGCCTATGACGTGGCCATCAGCCA
TTGGATGGGCGTGCGGCTGAGCGAGCAAGCTTCCCAATGGCTTGAGGCTATTCCCCTTAGGCAGAGGCTGCGCTATGGCG
AGAATCCCCATCAGCAGGCGGCTTGGTACAGCGCCCCGAAGCAGGGTTGGGGCGGTGCCATTCAGTTGCAGGGCAAGGAG
CTCAGTACCAACAATTTGCTTGATTTGGAAGCTGCTCTGGCCACTGTTCGGGAGTTTGGATATGGCACGGATGGGGCTCA
TCAAGCTGTTCAAGATGCTGCCGTGGTGGTTAAGCACACCAACCCTTGTGGCGTCGCGATTGGCACTGGCGTGGCGTCGG
CCTTAAGCCGTGCCTTGGATGCGGATCGGGTGAGTGCATTTGGAGGGATCGTGGCTCTGAATGGATTAGTGGATGCCACG
ACTGCTCGGGAGCTCACCAGTTTGTTCTTGGAGTGTGTTGTGGCACCAGGCTTTGAACCAGAAGCTCGGGAAATCCTTGC
CAGCAAGGCCAACTTACGCTTGTTGGAATTGGCTCCAGGTGCCGTTGATGCGGCTGGTCGTGATCACATTCGCACCATCC
TCGGCGGCGTGTTGGTGCAGGACCAAGACGATCAATTGATTGATCCCACCAGTTGGACTGTGGCCAGCAAACGCGCGCCT
ACTGCTGAAGAAAACGATGACCTCACCTTTGCGTGGCGCCTGGTGAGACATGTGCGCTCTAACGCCATCGTGGTGGCTCG
TGCTGGCCAGAGTCTCGGAGTTGGAGCGGGCCAGATGAACCGGGTCGGCTCGGCGCGACTAGCCCTTGAGGCAGCTGGCG
ATCAAGCCAGGGGAGCTGTTTTGGCTAGTGATGGCTTCTTCCCCTTTGACGACACGGTGCGTTTGGCGGCTAACCATGGC
ATACGTGCTGTGATCCAGCCTGGTGGGAGCAAGCGTGATGCAGATTCAATCGCTGTTTGCGATGAATTTGGTCTGGCAAT
GGTGTTAACAGGAAAGCGCCACTTCTTGCATTAA

Upstream 100 bases:

>100_bases
AAGCACCTTTGGGAATGTCGCGGAGAAGGTTGTCGCTCACGGCAGGGGGTGTATCGCTGCTGCGTAGGTTGGCCCACGGA
ATGCTTTCGTCTCTGCTGCC

Downstream 100 bases:

>100_bases
GTTAGTAATAATCAAAGCTAGTATAGAGCTACTTTGTTGCCCTATATTTTAACTTTATTGATTTCCTTGATGAGTTTCAT
TCTGACTGAATTTGCTTTGG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MAPIALLSVSNKHGIVSLAESLHRMHGFQLLSSGGTAKVLEDAGLPVTRVAEHTGAAEILGGRVKTLHPRVHGGILAMRG
DPDHEVDLEQHQIPPIDVVVVNLYPFRETVANPQVSWETAIENIDIGGPAMVRAAAKNHAHVAVLTRPDQYDRFLVALSD
GVDGQLRRELALEAFEHTAAYDVAISHWMGVRLSEQASQWLEAIPLRQRLRYGENPHQQAAWYSAPKQGWGGAIQLQGKE
LSTNNLLDLEAALATVREFGYGTDGAHQAVQDAAVVVKHTNPCGVAIGTGVASALSRALDADRVSAFGGIVALNGLVDAT
TARELTSLFLECVVAPGFEPEAREILASKANLRLLELAPGAVDAAGRDHIRTILGGVLVQDQDDQLIDPTSWTVASKRAP
TAEENDDLTFAWRLVRHVRSNAIVVARAGQSLGVGAGQMNRVGSARLALEAAGDQARGAVLASDGFFPFDDTVRLAANHG
IRAVIQPGGSKRDADSIAVCDEFGLAMVLTGKRHFLH

Sequences:

>Translated_517_residues
MAPIALLSVSNKHGIVSLAESLHRMHGFQLLSSGGTAKVLEDAGLPVTRVAEHTGAAEILGGRVKTLHPRVHGGILAMRG
DPDHEVDLEQHQIPPIDVVVVNLYPFRETVANPQVSWETAIENIDIGGPAMVRAAAKNHAHVAVLTRPDQYDRFLVALSD
GVDGQLRRELALEAFEHTAAYDVAISHWMGVRLSEQASQWLEAIPLRQRLRYGENPHQQAAWYSAPKQGWGGAIQLQGKE
LSTNNLLDLEAALATVREFGYGTDGAHQAVQDAAVVVKHTNPCGVAIGTGVASALSRALDADRVSAFGGIVALNGLVDAT
TARELTSLFLECVVAPGFEPEAREILASKANLRLLELAPGAVDAAGRDHIRTILGGVLVQDQDDQLIDPTSWTVASKRAP
TAEENDDLTFAWRLVRHVRSNAIVVARAGQSLGVGAGQMNRVGSARLALEAAGDQARGAVLASDGFFPFDDTVRLAANHG
IRAVIQPGGSKRDADSIAVCDEFGLAMVLTGKRHFLH
>Mature_516_residues
APIALLSVSNKHGIVSLAESLHRMHGFQLLSSGGTAKVLEDAGLPVTRVAEHTGAAEILGGRVKTLHPRVHGGILAMRGD
PDHEVDLEQHQIPPIDVVVVNLYPFRETVANPQVSWETAIENIDIGGPAMVRAAAKNHAHVAVLTRPDQYDRFLVALSDG
VDGQLRRELALEAFEHTAAYDVAISHWMGVRLSEQASQWLEAIPLRQRLRYGENPHQQAAWYSAPKQGWGGAIQLQGKEL
STNNLLDLEAALATVREFGYGTDGAHQAVQDAAVVVKHTNPCGVAIGTGVASALSRALDADRVSAFGGIVALNGLVDATT
ARELTSLFLECVVAPGFEPEAREILASKANLRLLELAPGAVDAAGRDHIRTILGGVLVQDQDDQLIDPTSWTVASKRAPT
AEENDDLTFAWRLVRHVRSNAIVVARAGQSLGVGAGQMNRVGSARLALEAAGDQARGAVLASDGFFPFDDTVRLAANHGI
RAVIQPGGSKRDADSIAVCDEFGLAMVLTGKRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=493, Percent_Identity=38.9452332657201, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1790439, Length=535, Percent_Identity=46.3551401869159, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71985564, Length=482, Percent_Identity=37.1369294605809, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=327, Percent_Identity=29.9694189602446, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323056, Length=475, Percent_Identity=39.1578947368421, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=39.0852390852391, Blast_Score=276, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24649832, Length=484, Percent_Identity=40.495867768595, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PROM3 (A2CCK6)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018481.1
- ProteinModelPortal:   A2CCK6
- SMR:   A2CCK6
- STRING:   A2CCK6
- GeneID:   4776758
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_24851
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55151; Mature: 55020

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPIALLSVSNKHGIVSLAESLHRMHGFQLLSSGGTAKVLEDAGLPVTRVAEHTGAAEIL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCCEEEHHHCCCCHHHHHHHCCHHHHH
GGRVKTLHPRVHGGILAMRGDPDHEVDLEQHQIPPIDVVVVNLYPFRETVANPQVSWETA
CCCCEEECCCCCCCEEEECCCCCCCCCCHHCCCCCEEEEEEEEECHHHHHCCCCCCHHHH
IENIDIGGPAMVRAAAKNHAHVAVLTRPDQYDRFLVALSDGVDGQLRRELALEAFEHTAA
HCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
YDVAISHWMGVRLSEQASQWLEAIPLRQRLRYGENPHQQAAWYSAPKQGWGGAIQLQGKE
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCC
LSTNNLLDLEAALATVREFGYGTDGAHQAVQDAAVVVKHTNPCGVAIGTGVASALSRALD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHC
ADRVSAFGGIVALNGLVDATTARELTSLFLECVVAPGFEPEAREILASKANLRLLELAPG
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCC
AVDAAGRDHIRTILGGVLVQDQDDQLIDPTSWTVASKRAPTAEENDDLTFAWRLVRHVRS
CCCCCCHHHHHHHHCCEEEECCCCCEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCC
NAIVVARAGQSLGVGAGQMNRVGSARLALEAAGDQARGAVLASDGFFPFDDTVRLAANHG
CEEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEECCCC
IRAVIQPGGSKRDADSIAVCDEFGLAMVLTGKRHFLH
EEEEECCCCCCCCCCCEEEHHCCCEEEEEECCHHCCC
>Mature Secondary Structure 
APIALLSVSNKHGIVSLAESLHRMHGFQLLSSGGTAKVLEDAGLPVTRVAEHTGAAEIL
CCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCCEEEHHHCCCCHHHHHHHCCHHHHH
GGRVKTLHPRVHGGILAMRGDPDHEVDLEQHQIPPIDVVVVNLYPFRETVANPQVSWETA
CCCCEEECCCCCCCEEEECCCCCCCCCCHHCCCCCEEEEEEEEECHHHHHCCCCCCHHHH
IENIDIGGPAMVRAAAKNHAHVAVLTRPDQYDRFLVALSDGVDGQLRRELALEAFEHTAA
HCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
YDVAISHWMGVRLSEQASQWLEAIPLRQRLRYGENPHQQAAWYSAPKQGWGGAIQLQGKE
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCC
LSTNNLLDLEAALATVREFGYGTDGAHQAVQDAAVVVKHTNPCGVAIGTGVASALSRALD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHC
ADRVSAFGGIVALNGLVDATTARELTSLFLECVVAPGFEPEAREILASKANLRLLELAPG
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCC
AVDAAGRDHIRTILGGVLVQDQDDQLIDPTSWTVASKRAPTAEENDDLTFAWRLVRHVRS
CCCCCCHHHHHHHHCCEEEECCCCCEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCC
NAIVVARAGQSLGVGAGQMNRVGSARLALEAAGDQARGAVLASDGFFPFDDTVRLAANHG
CEEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEECCCC
IRAVIQPGGSKRDADSIAVCDEFGLAMVLTGKRHFLH
EEEEECCCCCCCCCCCEEEHHCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA