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The map label for this gene is glyA [H]

Identifier: 124024161

GI number: 124024161

Start: 2173406

End: 2174680

Strand: Reverse

Name: glyA [H]

Synonym: P9303_24721

Alternate gene names: 124024161

Gene position: 2174680-2173406 (Counterclockwise)

Preceding gene: 124024166

Following gene: 124024160

Centisome position: 81.06

GC content: 55.61

Gene sequence:

>1275_bases
ATGACGGATCGCTGCTTGGCTTCTATTAATGCTGCCCTGACGGATTCCGACCCGGCGATTGCAGGCTTGATCGATCAGGA
GAGGCAACGTCAGGAGACCCATCTAGAGCTGATAGCCAGTGAGAACTTCACTTCTCAAGCTGTGATGCAGGCTCAGGGTT
CTGTGCTCACTAACAAGTACGCCGAAGGTCTTCCCCATAAGCGCTATTACGGCGGTTGTGAGCATGTCGATGCGATTGAG
GAGTTGGCGATCGAGCGTGCTCGGCGGCTCTTTGGTGCGGCTTGGGCCAACGTGCAACCTCATAGTGGCGCGCAGGCCAA
CTTCGCTGTCTTCCTGGCGCTTTTGCAGCCTGGTGACACCATCATGGGGATGGATCTATCCCATGGGGGGCATCTCACTC
ATGGTTCTCCTGTCAATGTCAGCGGCAAGTGGTTCAAGGTCGTTCATTACGGCGTGGAGCCTGATAGTCAGCAGCTCGAC
ATGGAGGCTGTGCGTCAGCTGGCCCTGAAAGAGCGTCCTCAACTGATTATCTGCGGCTACTCGGCCTATCCCCGCACGAT
CGATTTTGCTGCTTTTCGCAGCATCGCTGATGAGGTGGGCGCTTACTTGTTGGCGGACATGGCACACATTGCCGGCCTTG
TTGCGGCCGGGGTCCATCCGAGTCCTATCGCCCACTGTGATGTGGTGACGACGACCACCCACAAGACATTGCGTGGCCCC
AGAGGCGGCTTAATCCTTTGTCGTGATGCGGACTTTGGCCGTAAGTTTGACAAGGCAGTGTTTCCTGGCAGTCAGGGCGG
CCCTTTGGAACATGTGATCGCAGCCAAGGCTGTGGCCTTAGGAGAAGCTCTTCAGCCAGAGTTCCATGCTTATAGCTGCC
AGGTGGTGGCGAATGCCCAGGTTCTTGCTGGTCGCATTCAGGAGCGCGGGATTGCGGTTGTGAGTGGAGGAACAGATAAT
CACCTTGTCTTGCTGGATCTGCGCAGCATTGGGATGACCGGGAAGGTTGCTGATTTGCTTGTCAGTGACGTAAACATCAC
CGCCAATAAGAACACAGTGCCCTTTGATCCTGAGTCGCCTTTTGTGACCAGTGGTCTGCGCTTGGGCACGGCGGCTCTAA
CAACTCGTGGCTTTGATGAGGAGGCATTCCGTGAGGTTGCTGATGTGATTGCAGATCGTTTGCTTAAACCGCAAGATGAA
TCGATTAAGGCGCAGTGCTTGGAACGGGTGAGACAACTTTGTGGCCGTTTCCCTCTCTACAGGGGTTCCCTCTAG

Upstream 100 bases:

>100_bases
TTTTTTTGCACTTGGTTTCGGCGGCGGTCTTAGGGCTAAGGGCTGTTCTTAAGACGTGAAGAACCATTGCCTACGATCCG
AAAACTGTTGGATTCTGTAA

Downstream 100 bases:

>100_bases
CCAGCGCTTGCTTGACTGGCCCTTCGCTCTGGAATCACAGGTTTGCTCTGCCTAGGATTCATTCCATCGGCCCTTCTTTA
GGTCGCTTGCTACAGGAGCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTDRCLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIE
ELAIERARRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVEPDSQQLD
MEAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFHAYSCQVVANAQVLAGRIQERGIAVVSGGTDN
HLVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDEEAFREVADVIADRLLKPQDE
SIKAQCLERVRQLCGRFPLYRGSL

Sequences:

>Translated_424_residues
MTDRCLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIE
ELAIERARRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVEPDSQQLD
MEAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFHAYSCQVVANAQVLAGRIQERGIAVVSGGTDN
HLVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDEEAFREVADVIADRLLKPQDE
SIKAQCLERVRQLCGRFPLYRGSL
>Mature_423_residues
TDRCLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKYAEGLPHKRYYGGCEHVDAIEE
LAIERARRLFGAAWANVQPHSGAQANFAVFLALLQPGDTIMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVEPDSQQLDM
EAVRQLALKERPQLIICGYSAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGPR
GGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFHAYSCQVVANAQVLAGRIQERGIAVVSGGTDNH
LVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDEEAFREVADVIADRLLKPQDES
IKAQCLERVRQLCGRFPLYRGSL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=51.9900497512438, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=51.9900497512438, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=51.9900497512438, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=51.9900497512438, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=397, Percent_Identity=52.6448362720403, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=49.1271820448878, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=47.8036175710594, Blast_Score=308, Evalue=9e-84,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=57.3170731707317, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=48.7373737373737, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=48.7373737373737, Blast_Score=357, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=445, Percent_Identity=42.9213483146067, Blast_Score=356, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6323087, Length=405, Percent_Identity=42.962962962963, Blast_Score=332, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24640005, Length=412, Percent_Identity=47.5728155339806, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=412, Percent_Identity=47.5728155339806, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45740; Mature: 45608

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRCLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKY
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCHHHHHH
AEGLPHKRYYGGCEHVDAIEELAIERARRLFGAAWANVQPHSGAQANFAVFLALLQPGDT
HHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCE
IMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVEPDSQQLDMEAVRQLALKERPQLIICGY
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHCCCCEEEECC
SAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCC
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFHAYSCQVVANAQ
CCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHH
VLAGRIQERGIAVVSGGTDNHLVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCC
FVTSGLRLGTAALTTRGFDEEAFREVADVIADRLLKPQDESIKAQCLERVRQLCGRFPLY
CHHCCCHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
RGSL
CCCC
>Mature Secondary Structure 
TDRCLASINAALTDSDPAIAGLIDQERQRQETHLELIASENFTSQAVMQAQGSVLTNKY
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCHHHHHH
AEGLPHKRYYGGCEHVDAIEELAIERARRLFGAAWANVQPHSGAQANFAVFLALLQPGDT
HHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCE
IMGMDLSHGGHLTHGSPVNVSGKWFKVVHYGVEPDSQQLDMEAVRQLALKERPQLIICGY
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHCCHHHHHHHHHHCCCCEEEECC
SAYPRTIDFAAFRSIADEVGAYLLADMAHIAGLVAAGVHPSPIAHCDVVTTTTHKTLRGP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCC
RGGLILCRDADFGRKFDKAVFPGSQGGPLEHVIAAKAVALGEALQPEFHAYSCQVVANAQ
CCCEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHH
VLAGRIQERGIAVVSGGTDNHLVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCC
FVTSGLRLGTAALTTRGFDEEAFREVADVIADRLLKPQDESIKAQCLERVRQLCGRFPLY
CHHCCCHHCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
RGSL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]