Click here to switch to the map view.

The map label for this gene is truA

Identifier: 124024021

GI number: 124024021

Start: 2047471

End: 2048358

Strand: Direct

Name: truA

Synonym: P9303_23281

Alternate gene names: 124024021

Gene position: 2047471-2048358 (Clockwise)

Preceding gene: 124024020

Following gene: 124024022

Centisome position: 76.32

GC content: 54.39

Gene sequence:

>888_bases
ATGTTCCCTAACTCCAAAGTCGAACTGCCAAAAAGTACGCCAGTTCGACGTATTGGCCTCAGCGTGCAATATGAAGGTTC
AAACTTTTGCGGTTGGCAGCGTCAACCACAAGCGGTGAGCGTTCAACAAGTACTTGAAGAGGCAATCTCCCAACTTGATC
CTCAGAGGCCTATTCAGGCCGTTGCAGCAGGTCGCACAGATGCTGGTGTCCATGCTGCGGGCCAAGTTGTGCACTTCGAC
TGCTGTGGTCCTATCCCAGCTGAACGCTGGGCACCTGCTCTAAACGGACGACTACCTAACACGATTCGTGTGCGGGAAGC
AGTAGCACGCCCAGCCTCCTGGCATGCCTGTCATTCAGCCACATACCGAAGATATCGCTACACGATCTACAACGGTCGGC
GACCCAATCTGTTCCTCACCCCCTGGAGCTGGCACCGCTACCACGTTCGTCTTGATGAAACCGTGATGCAAGTGGCTCTA
GAGGGGCTCCTTGGGTTGCAAGACCTCAGCGCTTTTCAACGGACTGGCAGTCGACGAGCCCATGCCCGCACCACAGTTCA
GGACGTAAAGATTGAGCGGCAGGGAGATTTAATAATGATTGAGATCCAAGCCAGCGGCTTCCTCTATGGCATGGTGCGGC
TGTTGATGGGCCAATTGGTGGCCTTAGGCGAACACCGACTTAATCTTCAGACCTTTGAGCGGCGCTGGAAGGAGAAACGC
CGTCAAGAAGTGAAAGAAGCGGCACCAGCTCAGGGTCTTTGCCTCCTGCGTGCAGGATACGAAGAAGACATTTTCTCTAA
AGGCGGTTGGTATGACTGCCAACCGCGCTATTCCCTCGGAGCAAATGATCCTCCTTTGGATCCTCCATCTGCACCGGAGT
CAAGCTGA

Upstream 100 bases:

>100_bases
TCATCCGTACGGTGCCTCGCCGCGGCGACAATGCTGAGATGGCAATCATAGAGTTGGTCTAAAGCCTGGGAACAGTGACA
AAATGCTGAACTGATCAGCA

Downstream 100 bases:

>100_bases
ACTCCCACCACCACGCCTCACCCCTAGGGCAAACACAAGGGCTTCGCGAGCCATTCCATGAGTGATCTTTTTGGATTTGC
TCCCATGAAAGGCTAAACTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEEAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPNTIRVREAVARPASWHACHSATYRRYRYTIYNGRRPNLFLTPWSWHRYHVRLDETVMQVAL
EGLLGLQDLSAFQRTGSRRAHARTTVQDVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLQTFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS

Sequences:

>Translated_295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEEAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPNTIRVREAVARPASWHACHSATYRRYRYTIYNGRRPNLFLTPWSWHRYHVRLDETVMQVAL
EGLLGLQDLSAFQRTGSRRAHARTTVQDVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLQTFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
>Mature_295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEEAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPNTIRVREAVARPASWHACHSATYRRYRYTIYNGRRPNLFLTPWSWHRYHVRLDETVMQVAL
EGLLGLQDLSAFQRTGSRRAHARTTVQDVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLQTFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=34.0579710144928, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=36.9477911646586, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PROM3 (A2CC53)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018328.1
- ProteinModelPortal:   A2CC53
- SMR:   A2CC53
- STRING:   A2CC53
- GeneID:   4777177
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_23281
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 33342; Mature: 33342

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEEAISQLDPQRPIQA
CCCCCCEECCCCCCHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VAAGRTDAGVHAAGQVVHFDCCGPIPAERWAPALNGRLPNTIRVREAVARPASWHACHSA
HHCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCCCHHHHHHH
TYRRYRYTIYNGRRPNLFLTPWSWHRYHVRLDETVMQVALEGLLGLQDLSAFQRTGSRRA
HHHEEEEEEECCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCHH
HARTTVQDVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLQTFERRWKEKR
HHHHHHHHEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
HHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEEAISQLDPQRPIQA
CCCCCCEECCCCCCHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VAAGRTDAGVHAAGQVVHFDCCGPIPAERWAPALNGRLPNTIRVREAVARPASWHACHSA
HHCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCCCHHHHHHH
TYRRYRYTIYNGRRPNLFLTPWSWHRYHVRLDETVMQVALEGLLGLQDLSAFQRTGSRRA
HHHEEEEEEECCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCHH
HARTTVQDVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLQTFERRWKEKR
HHHHHHHHEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
HHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA