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The map label for this gene is metK

Identifier: 124023911

GI number: 124023911

Start: 1967800

End: 1969059

Strand: Direct

Name: metK

Synonym: P9303_22181

Alternate gene names: 124023911

Gene position: 1967800-1969059 (Clockwise)

Preceding gene: 124023910

Following gene: 124023912

Centisome position: 73.35

GC content: 55.63

Gene sequence:

>1260_bases
ATGAGCCGTTACGTCTTCACCTCTGAGTCCGTCACAGAAGGGCATCCCGACAAGATCTGTGATCAAGTGAGTGATGCCGT
TCTAGACGCCCTACTCGCCCAAGACCCGAGCAGCCGAGTTGCCTGTGAAACAGTTGTCAATACAGGGCTCTGCCTCATCA
CAGGCGAAGTGACCTCAAACTCCCAGGTTGATTTCATCCATCTTGTTAGGAACGTAATCCGCGAGATTGGCTATAGCGGT
GCTCGTGCAGGTGGGTTTGATGCCACCAGTTGCGCCGTGCTCGTTGCCCTGGATCAACAATCACCAGACATCGCCCAAGG
GGTTAATGAAGCCGACGACCATTCAGGTGATCCTCTTGACAAGGTGGGAGCCGGAGACCAAGGGATCATGTTTGGCTACG
CCTGCGACGACACCCCTGAGCTGATGCCCCTACCCATCAGCCTCGCCCATCGCCTTGCCCGTCAACTCGCTGCAGTCCGT
CATGACGGGTCTCTGAGCTACTTGCTACCTGATGGAAAGACCCAAGTCAGTGTGGTCTATGAAAACGACCGTCCTGTCGC
GATCGACACGATTTTGATCTCCACTCAACACACCTCGGAGGTAGAGGGCATCTCGGATGAGAATGGAATCCGTGAGCGGA
TCACTCAAGACCTCTGGACCCATGTCGTAGAACCAGCGACTGCTGACCTCCCCCTCAAACCCAATCGTGAGGCGACCCGC
TTTTTAGTCAATCCAACCGGTAAGTTTGTGGTCGGAGGCCCACAGGGAGATGCTGGCCTTACTGGCCGAAAGATCATCGT
AGATACCTACGGCGGCTATGCCCGCCATGGTGGTGGAGCCTTTTCCGGCAAAGACCCAACCAAAGTGGACCGCTCTGCCG
CCTACGCCGCACGATTTGTGGCCAAGTGTCTTGTAGCAGCAGGCCTCGCTCAACGTGCAGAGGTGCAACTCAGCTATGCC
ATTGGCGTGGCTAAACCCGTCTCGATCCTAGTGGAATCTTTTGGCACCGGAAAAGTGACGAATGACGAACTCACAGCCTT
GGTGCAAGACCATTTTGACCTGCGACCTGGTGCCATCATCGAACAATTCAAGCTGCGACAGCTGCCTCAGCAACGCGGAG
GACGGTTCTACCAAGACACTGCTGCCTATGGCCACTTCGGCCGCCCTGATCTAAATCTTCCCTGGGAAGACGTTACGGAC
AAGGCCTCCACTCTGCTTCAAGCAGAGGCCCAACAACAAAAACAGGGAAGCAGCCTCTAG

Upstream 100 bases:

>100_bases
ATCAACCCCCAACACTGACGAACTACCACCACCTCATCCACCACTGGAATGATCTAGTCGTCAAGGCAGACCCTAAAATT
ACGCATAACTTCAGTTCTCC

Downstream 100 bases:

>100_bases
AAACCCCATGCCTAACTCGTCCCTGGCGCTCGGCATCGACCTTGGCACCAGCGGCGTACGGCTTGCCGTGCTCAACGAGC
ACGGCAAGCTGATCCACACA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNSQVDFIHLVRNVIREIGYSG
ARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDDTPELMPLPISLAHRLARQLAAVR
HDGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYA
IGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLNLPWEDVTD
KASTLLQAEAQQQKQGSSL

Sequences:

>Translated_419_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNSQVDFIHLVRNVIREIGYSG
ARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDDTPELMPLPISLAHRLARQLAAVR
HDGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYA
IGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLNLPWEDVTD
KASTLLQAEAQQQKQGSSL
>Mature_418_residues
SRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNSQVDFIHLVRNVIREIGYSGA
RAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDDTPELMPLPISLAHRLARQLAAVRH
DGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYAI
GVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLNLPWEDVTDK
ASTLLQAEAQQQKQGSSL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=53.6523929471033, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=53.904282115869, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1789311, Length=408, Percent_Identity=55.8823529411765, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=52.6315789473684, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=52.7638190954774, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=399, Percent_Identity=52.8822055137845, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=53.6340852130326, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=47.7386934673367, Blast_Score=328, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=371, Percent_Identity=48.5175202156334, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=50.997150997151, Blast_Score=307, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=324, Percent_Identity=50.3086419753086, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=101, Percent_Identity=55.4455445544555, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=75, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=397, Percent_Identity=51.8891687657431, Blast_Score=391, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=51.6372795969773, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=50.5025125628141, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=50, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=50, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=49.0909090909091, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=53.2258064516129, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PROM3 (A2CBU3)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018218.1
- ProteinModelPortal:   A2CBU3
- SMR:   A2CBU3
- STRING:   A2CBU3
- GeneID:   4778066
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_22181
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44960; Mature: 44829

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSN
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCC
SQVDFIHLVRNVIREIGYSGARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLD
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHH
KVGAGDQGIMFGYACDDTPELMPLPISLAHRLARQLAAVRHDGSLSYLLPDGKTQVSVVY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
ENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
ECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKCLVAAGLAQRAEVQLSYAIGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHH
EQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLNLPWEDVTDKASTLLQAEAQQQKQGSSL
HHHHHHHCHHHHCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSN
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCC
SQVDFIHLVRNVIREIGYSGARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLD
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHH
KVGAGDQGIMFGYACDDTPELMPLPISLAHRLARQLAAVRHDGSLSYLLPDGKTQVSVVY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
ENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
ECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKCLVAAGLAQRAEVQLSYAIGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHH
EQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLNLPWEDVTDKASTLLQAEAQQQKQGSSL
HHHHHHHCHHHHCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA