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The map label for this gene is mdeA [H]

Identifier: 124023812

GI number: 124023812

Start: 1878661

End: 1880130

Strand: Direct

Name: mdeA [H]

Synonym: P9303_21191

Alternate gene names: 124023812

Gene position: 1878661-1880130 (Clockwise)

Preceding gene: 124023811

Following gene: 124023813

Centisome position: 70.03

GC content: 59.73

Gene sequence:

>1470_bases
GTGAGCCCACGCGACCTACTACAGGATCCCTGCTGGCACGCTGATGACCTCGGCAAAGCTCTACCAGATAGTCCCCATGC
CGTTTCGGTAGCCCTCCCCCGCTGGCAAGACGTGGTCGCCTATGAGGAGAAAGACCCTGCTTGTCTCAAGGCCCTGCAGG
CCATCTACCCCCGTTTCGGCTTCCATCCACTGGTGGCAGAGATCGCCCAAAAAGCACGAGAAGAGCATGGCTGTGCAGAT
GGCAGTGCCTGGCCCTATCCCACTGCTGCAGCCGCTGAGCGAGCCAGGTTTCATTGCCAGCGCAGTGAGCCGATGGCAAA
AACAACCATCAAAACCATACTGGGCCTGCCATGTTTGCTCACCGACGCAAAAGGCTGCAGCGCTGCCAAGGCCTTCTGGC
AACACAGCGGGCTTGGTGCTTCCTCACGTCAGGCTGCTAGCGCACTTGGGCTCGAACCATTCCCCTCAGTGCAAGCCAGC
GCGGACGCCCAGACGTTGCTAAGACAACGACTCGCAGAGATCTATGGCTGCGACCCAGAGCTCGTGCAGCTGCATCCCTC
TGGGATGGCTGCTCTCACCAAAGCACTGGAAGCGATTACGGCTCTACGCCCAGGCAAACCCTGCTTGCAGCTTGGCTTCC
CCTATGTGGATGTGCTCAAGCTGCCTGAGGTGGTGTTTGAAGGTTGCGACCTTCTACTGACAACAGAGCTGAACCGATTG
GCCCTGGAGTTCGATCGCAGACAACCAGCCGCTGTGATCGTTGAGCTACCCAGCAACCCCATGCTGCAGTGTGTCGACCT
GCCAGCGGTAGCCCGCCTAGCACAGGCTCGTGGTATCCCCGTGATCGCTGATGACACCATCGGCTCACCACTCAATCTTG
ATGCCCTGCCCCATGCAGACCTGGTGTTTAGCTCCCTCACCAAAAGCTTCGCCGGCCGTGGTGACATCCTGGCGGGTTCT
CTCGTTGTAAGCCCCAACTCCCGCTGGCGAGAGGAGCTGTCCAGAGAGCTCTCGACTGCCCTTGCTCCCCTCGGGGATGC
TGATGCCATCGCCCTAGAACAAGCCAGCCGAGATGTGGCCCAGCGGCTGCCGCAGCTCAACAACGCCTGTCAATATTTGG
CAGGCAAACTGGCAAGCCATCCTGCCGTTACTCGCGTCCACCATCCAGGCCAATGCGCCAACTTCCAAGCCCTGATGCGC
CCTGGCGCCGGCCACGGCTGCCTGCTCTCTTTTGAGTTATCGGGGGGGGTAGCGCAAGCAAAGCGGGTGTATGACGCCCT
GCAGGTCTGCAAAGGTCCAAGCCTCGGCACAAGCTTCACGCTGGTGTGCCCCTATGTACTGCTCGCCCATTACAACGAAC
TGAGCTGGGCAGAATCCTGTGGAGTGCCGAGCCACCTGCTGCGTGTATCTGTAGGGCTTGAAGATCCCGATACCCTTTGG
CAACGCTTTGAGATGGCCTTCAAGGCCTAA

Upstream 100 bases:

>100_bases
GAAAACTTGGCATCGATGATGGTCTGGTGCGCTTCTCCGTGGGCTGCGAAGACCTCGCTGATCTCAGCACAGATTTAGAT
CAAGCCCTCAAACAGCTGTA

Downstream 100 bases:

>100_bases
CCGTAAGCAACCCATCACATGGTCGTCACAACAGATCATCGTCCTCACCAATAGAGCCAAATTCCAGCTAATGATGTGGT
CGATTCATAAGCACGCATGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSPRDLLQDPCWHADDLGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKAREEHGCAD
GSAWPYPTAAAAERARFHCQRSEPMAKTTIKTILGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGLEPFPSVQAS
ADAQTLLRQRLAEIYGCDPELVQLHPSGMAALTKALEAITALRPGKPCLQLGFPYVDVLKLPEVVFEGCDLLLTTELNRL
ALEFDRRQPAAVIVELPSNPMLQCVDLPAVARLAQARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAGS
LVVSPNSRWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQYLAGKLASHPAVTRVHHPGQCANFQALMR
PGAGHGCLLSFELSGGVAQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDTLW
QRFEMAFKA

Sequences:

>Translated_489_residues
MSPRDLLQDPCWHADDLGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKAREEHGCAD
GSAWPYPTAAAAERARFHCQRSEPMAKTTIKTILGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGLEPFPSVQAS
ADAQTLLRQRLAEIYGCDPELVQLHPSGMAALTKALEAITALRPGKPCLQLGFPYVDVLKLPEVVFEGCDLLLTTELNRL
ALEFDRRQPAAVIVELPSNPMLQCVDLPAVARLAQARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAGS
LVVSPNSRWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQYLAGKLASHPAVTRVHHPGQCANFQALMR
PGAGHGCLLSFELSGGVAQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDTLW
QRFEMAFKA
>Mature_488_residues
SPRDLLQDPCWHADDLGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKAREEHGCADG
SAWPYPTAAAAERARFHCQRSEPMAKTTIKTILGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGLEPFPSVQASA
DAQTLLRQRLAEIYGCDPELVQLHPSGMAALTKALEAITALRPGKPCLQLGFPYVDVLKLPEVVFEGCDLLLTTELNRLA
LEFDRRQPAAVIVELPSNPMLQCVDLPAVARLAQARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAGSL
VVSPNSRWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQYLAGKLASHPAVTRVHHPGQCANFQALMRP
GAGHGCLLSFELSGGVAQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDTLWQ
RFEMAFKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=247, Percent_Identity=29.9595141700405, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71980708, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=565, Percent_Identity=28.4955752212389, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322590, Length=443, Percent_Identity=30.9255079006772, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323557, Length=346, Percent_Identity=34.6820809248555, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=281, Percent_Identity=26.3345195729537, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922554, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 52604; Mature: 52473

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRDLLQDPCWHADDLGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFG
CCCHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCC
FHPLVAEIAQKAREEHGCADGSAWPYPTAAAAERARFHCQRSEPMAKTTIKTILGLPCLL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEE
TDAKGCSAAKAFWQHSGLGASSRQAASALGLEPFPSVQASADAQTLLRQRLAEIYGCDPE
ECCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
LVQLHPSGMAALTKALEAITALRPGKPCLQLGFPYVDVLKLPEVVFEGCDLLLTTELNRL
HEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHH
ALEFDRRQPAAVIVELPSNPMLQCVDLPAVARLAQARGIPVIADDTIGSPLNLDALPHAD
HHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHH
LVFSSLTKSFAGRGDILAGSLVVSPNSRWREELSRELSTALAPLGDADAIALEQASRDVA
HHHHHHHHHHCCCCCEEECEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QRLPQLNNACQYLAGKLASHPAVTRVHHPGQCANFQALMRPGAGHGCLLSFELSGGVAQA
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHH
KRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDTLW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHEEECCCCCHHHHH
QRFEMAFKA
HHHHHHHCC
>Mature Secondary Structure 
SPRDLLQDPCWHADDLGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFG
CCHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCC
FHPLVAEIAQKAREEHGCADGSAWPYPTAAAAERARFHCQRSEPMAKTTIKTILGLPCLL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEE
TDAKGCSAAKAFWQHSGLGASSRQAASALGLEPFPSVQASADAQTLLRQRLAEIYGCDPE
ECCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHH
LVQLHPSGMAALTKALEAITALRPGKPCLQLGFPYVDVLKLPEVVFEGCDLLLTTELNRL
HEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHH
ALEFDRRQPAAVIVELPSNPMLQCVDLPAVARLAQARGIPVIADDTIGSPLNLDALPHAD
HHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHH
LVFSSLTKSFAGRGDILAGSLVVSPNSRWREELSRELSTALAPLGDADAIALEQASRDVA
HHHHHHHHHHCCCCCEEECEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QRLPQLNNACQYLAGKLASHPAVTRVHHPGQCANFQALMRPGAGHGCLLSFELSGGVAQA
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHH
KRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDTLW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHEEECCCCCHHHHH
QRFEMAFKA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]