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The map label for this gene is metB [H]

Identifier: 124023811

GI number: 124023811

Start: 1877492

End: 1878661

Strand: Direct

Name: metB [H]

Synonym: P9303_21181

Alternate gene names: 124023811

Gene position: 1877492-1878661 (Clockwise)

Preceding gene: 124023809

Following gene: 124023812

Centisome position: 69.99

GC content: 55.98

Gene sequence:

>1170_bases
GTGGCCCCAGCTCAAAGCGATCAACAGCAGGAGGTTGCAGGGATCAGCACCCGCGCCATCCACCACGGCGAGAGCTTTGC
AGCAGAGACCGGCGCCGTGATGCCACCGATCTTTACCAGTTCAACCTTTGCCCACGGCAATCCTCAGGGCTTCGACTACA
CCCGTTCAGGCAATCCCAATTTCCGCATCCTCGAATCAGTGTTGGCAGCAGTGGAAAGCTGTCAATTCGCCACTGCCTTC
GCTTCAGGGGTGAGCGCCATCACAGCCATCGCCTCCACACTCCAAGCCGGAGATCTGGTGCTCTGTGAGGAAAATCTCTA
CGGCTGCACGGTGCGCTTATTCGAGCAGGTTTTTAGTCGTTTTGGTGTACAAACAGCCTGGCTGGATTTCACCAATCCAT
CAGCTCTTGATGCCATTGCAGTTCAAAAACCAGCAATGGTCTGGCTGGAGAGTCCAACCAATCCCCTGCTCAAAGTGATC
GATCTAGCAGCCGTCTGTGATGCCGCTGCAGCAGAAAAAATCCCAGTTGTCGTCGACAACACGTTTGCCACAGCCCTGCT
GCAACGACCACTGCAGCTAGGCGCCACCCTCTCACTCACCAGCACCACCAAATACATCAACGGCCATTCCGATGCCCTTG
GCGGTGTGGTCTGCAGCGACGACCCGCAATGGCAGCAAAAGATGGTGTTTGCACAAAAGGCCTTGGGATTACAACCCTCC
CCATTTGACGCCTGGTTGATTACTCGCGGCATCAAAACCCTGCCACTCAGGCTCGAACGCCAAATCACTAATGCTGCCGC
CATCGCCGATCAACTGGCCAGCCATCCACGCATGCACTGGGTGCGCTATCCATTCCGTTCAGACCATCCCCAGCAGGCTT
TAGCCCGCAAGCAGATGAGCGCGGGTGGTGCAATTGTCACGGTCAGTCTCGACGCTGACCAAAACCAGACTTACGCCTTC
TGCAAGAGCCTGCGCTATTTCACCATGGCCGAAAGCCTGGGCGGTGTTGAAAGCCTCGTTTGTCATCCCGCCACCATGAC
CCATGCCTCAGTCGATCCAGATGTCAAGCGAAAACTTGGCATCGATGATGGTCTGGTGCGCTTCTCCGTGGGCTGCGAAG
ACCTCGCTGATCTCAGCACAGATTTAGATCAAGCCCTCAAACAGCTGTAG

Upstream 100 bases:

>100_bases
AGGTTTGAATCAAATTCAGCCAAGCGTTCTTCAACAGGCCCCTGCTTTGCAGGGGTTTTTTGTTGCTGTTGCCTACCCTG
CTCAAAGCCCTCTCTAGATC

Downstream 100 bases:

>100_bases
TGAGCCCACGCGACCTACTACAGGATCCCTGCTGGCACGCTGATGACCTCGGCAAAGCTCTACCAGATAGTCCCCATGCC
GTTTCGGTAGCCCTCCCCCG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAPAQSDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATAF
ASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMVWLESPTNPLLKVI
DLAAVCDAAAAEKIPVVVDNTFATALLQRPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
PFDAWLITRGIKTLPLRLERQITNAAAIADQLASHPRMHWVRYPFRSDHPQQALARKQMSAGGAIVTVSLDADQNQTYAF
CKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSTDLDQALKQL

Sequences:

>Translated_389_residues
MAPAQSDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATAF
ASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMVWLESPTNPLLKVI
DLAAVCDAAAAEKIPVVVDNTFATALLQRPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
PFDAWLITRGIKTLPLRLERQITNAAAIADQLASHPRMHWVRYPFRSDHPQQALARKQMSAGGAIVTVSLDADQNQTYAF
CKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSTDLDQALKQL
>Mature_388_residues
APAQSDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATAFA
SGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMVWLESPTNPLLKVID
LAAVCDAAAAEKIPVVVDNTFATALLQRPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPSP
FDAWLITRGIKTLPLRLERQITNAAAIADQLASHPRMHWVRYPFRSDHPQQALARKQMSAGGAIVTVSLDADQNQTYAFC
KSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSTDLDQALKQL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=43.3862433862434, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=39.9470899470899, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=38.9920424403183, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=37.7659574468085, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789383, Length=343, Percent_Identity=25.9475218658892, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=43.6507936507937, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=43.9153439153439, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=32.1256038647343, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=40.0510204081633, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321254, Length=384, Percent_Identity=30.9895833333333, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=28.3980582524272, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.4615384615385, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=304, Percent_Identity=25.9868421052632, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=41.9270833333333, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41900; Mature: 41769

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAQSDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPN
CCCCCCCCHHHHHCCHHHHEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FRILESVLAAVESCQFATAFASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
FGVQTAWLDFTNPSALDAIAVQKPAMVWLESPTNPLLKVIDLAAVCDAAAAEKIPVVVDN
HCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECC
TFATALLQRPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
HHHHHHHHHHHHHCCEEEECCCHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCC
PFDAWLITRGIKTLPLRLERQITNAAAIADQLASHPRMHWVRYPFRSDHPQQALARKQMS
CCHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHC
AGGAIVTVSLDADQNQTYAFCKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCHHHHHHCC
IDDGLVRFSVGCEDLADLSTDLDQALKQL
CCCCEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APAQSDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPN
CCCCCCCHHHHHCCHHHHEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FRILESVLAAVESCQFATAFASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
FGVQTAWLDFTNPSALDAIAVQKPAMVWLESPTNPLLKVIDLAAVCDAAAAEKIPVVVDN
HCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECC
TFATALLQRPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
HHHHHHHHHHHHHCCEEEECCCHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCC
PFDAWLITRGIKTLPLRLERQITNAAAIADQLASHPRMHWVRYPFRSDHPQQALARKQMS
CCHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHC
AGGAIVTVSLDADQNQTYAFCKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCHHHHHHCC
IDDGLVRFSVGCEDLADLSTDLDQALKQL
CCCCEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]