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The map label for this gene is lepA [H]

Identifier: 124023787

GI number: 124023787

Start: 1848563

End: 1850377

Strand: Reverse

Name: lepA [H]

Synonym: P9303_20941

Alternate gene names: 124023787

Gene position: 1850377-1848563 (Counterclockwise)

Preceding gene: 124023788

Following gene: 124023780

Centisome position: 68.98

GC content: 50.3

Gene sequence:

>1815_bases
ATGACCGACGTTCCCGTTTCTCGCTTGAGGAACTTCTGCATCATTGCCCATATCGATCACGGCAAGTCGACTTTGGCTGA
CAGGCTGTTGCAGGACACTGGCACGGTGGCCGGTCGGGATATGCAGGAGCAGTTTCTCGACAACATGGATTTGGAAAGAG
AGCGGGGCATCACGATCAAGCTCCAGGCTGCCCGGATGAACTACACGGCTGCCGATGGCGAAAGCTATGTGCTCAACCTG
ATTGATACCCCTGGCCATGTGGATTTCTCCTATGAGGTGAGCCGCTCCTTGCAGGCCTGTGAAGGGGCCCTGTTGGTGGT
GGATGCCAGTCAGGGTGTTGAGGCCCAGACCCTGGCAAATGTCTATTTAGCGCTGGAAAATGATCTAGAGATTATTCCTG
TGCTCAACAAGATTGATCTGCCTGGCTCTGATCCAGAACGGATTAAAGAGGAGATCGAGGCGATCATTGGCCTTGACACC
TCAACAGCAATTGCCTGTTCTGCCAAAACAGGCTTAGGCGTATCAGAGATTATGCAGGCGGTGGTGGATCGCATCCCGCC
CCCTGCAGATACCCTCGATGAGCCAACGAGGGCATTGATCTTCGATTCCTACTACGACTCCTACCGAGGCGTGATTGTGT
ATTTCCGGGTGATCAGTGGTCGGATCAGCACGAAGGACAAAGTGTTGCTGATGGCCAGTAAGAAGAGCTATGAGCTTGAT
GAGATCGGTGTGATGTCACCAGATCAGTGTGAGGTCAATGAACTTCATGCCGGGGAGGTGGGTTATCTGGCGGCTTCGAT
CAAGGCGGTTGCTGATGCACGGGTTGGAGACACGATTACCCTCCTCAATGCCCCAGCAGATGAGCCGCTACCTGGTTACA
CCGAGGCAAAGCCGATGGTGTTTTGTGGCTTGTTCCCTACGGATGCAGATCAATATCCAGATCTAAGGGAAGCGCTTGAC
AAGCTGCAGCTTTCAGATGCAGCACTGAAGTATGAACCTGAAACCAGCAGTGCGATGGGTTTTGGCTTTCGTTGTGGCTT
TCTAGGACTCTTGCATATGGAAATTGTGCAGGAGCGGCTTGAACGTGAATACGACTTGGATTTGATTGTTACTGCTCCTT
CAGTGATCTATAAGGTGAACATGATTGATGGGCAGATGTTGATGGTGGATAACCCTGCAACATTGCCAGATCCGCAGAAG
CGGGAATCAATAGAGGAGCCTTATGTGCGGATGGAGATCTATGCCCCCAATGAGTACAACGGCACTCTGATGGGTTTGTG
TCAGGAACGACGTGGTGAATACATTGATATGAAGTACCTCACCACTGAGCGAGTCACCTTAATTTATGAGCTGCCCTTGG
CTGAAGTGGTGACGGATTTCTTTGATCAGATGAAGAGTCGCACAAAGGGTTATGCCTCAATGGAATATCACTTGATTGGC
TATCGCCGCAATGATCTGGTGCGTTTGGATGTCTTGATCAATGCAGAAAAGGCTGATCCACTTACCACAATTGCGCACCG
TGATAAGGCTTATAGCATTGGTAAGGGTCTGGTGGAGAAGCTCAAGGAGCTGATCCCAAGACAACAGTTTAAAATTCCCT
TACAGGCTTCCATTGGTAGTCGCATTATTGCCAGTGAGAGCATCAGTGCTATGCGCAAGGATGTGCTTGCTAAGTGTTAT
GGCGGAGACATCTCAAGGAAAAAAAAGTTGTTGAAGAAGCAGGCCAAGGGTAAAAAGCGGATGAAGGCCATGGGCAAAGT
GGATGTGCCTCAGGAAGCATTCATGGCTGTATTGAAGCTGAATCAAACGAGCTGA

Upstream 100 bases:

>100_bases
TGAGAGGGCTACCAGCATTCTTCATGGCTGGTCAGATCAGCAGGATCCTTAGAATCTTGATTCTGACTCCAAAGCGCCCG
AGAGGGGAATTCGCCAACGC

Downstream 100 bases:

>100_bases
TTTATCTTTTACTATTGACCATTATTTAATGCCTTTGCAGCGGCATGTTTGTCAACCTGTAGATAATTACCGTTGGTTTT
AGTTGATAAGTCTTCCATCC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MTDVPVSRLRNFCIIAHIDHGKSTLADRLLQDTGTVAGRDMQEQFLDNMDLERERGITIKLQAARMNYTAADGESYVLNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENDLEIIPVLNKIDLPGSDPERIKEEIEAIIGLDT
STAIACSAKTGLGVSEIMQAVVDRIPPPADTLDEPTRALIFDSYYDSYRGVIVYFRVISGRISTKDKVLLMASKKSYELD
EIGVMSPDQCEVNELHAGEVGYLAASIKAVADARVGDTITLLNAPADEPLPGYTEAKPMVFCGLFPTDADQYPDLREALD
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNMIDGQMLMVDNPATLPDPQK
RESIEEPYVRMEIYAPNEYNGTLMGLCQERRGEYIDMKYLTTERVTLIYELPLAEVVTDFFDQMKSRTKGYASMEYHLIG
YRRNDLVRLDVLINAEKADPLTTIAHRDKAYSIGKGLVEKLKELIPRQQFKIPLQASIGSRIIASESISAMRKDVLAKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQTS

Sequences:

>Translated_604_residues
MTDVPVSRLRNFCIIAHIDHGKSTLADRLLQDTGTVAGRDMQEQFLDNMDLERERGITIKLQAARMNYTAADGESYVLNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENDLEIIPVLNKIDLPGSDPERIKEEIEAIIGLDT
STAIACSAKTGLGVSEIMQAVVDRIPPPADTLDEPTRALIFDSYYDSYRGVIVYFRVISGRISTKDKVLLMASKKSYELD
EIGVMSPDQCEVNELHAGEVGYLAASIKAVADARVGDTITLLNAPADEPLPGYTEAKPMVFCGLFPTDADQYPDLREALD
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNMIDGQMLMVDNPATLPDPQK
RESIEEPYVRMEIYAPNEYNGTLMGLCQERRGEYIDMKYLTTERVTLIYELPLAEVVTDFFDQMKSRTKGYASMEYHLIG
YRRNDLVRLDVLINAEKADPLTTIAHRDKAYSIGKGLVEKLKELIPRQQFKIPLQASIGSRIIASESISAMRKDVLAKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQTS
>Mature_603_residues
TDVPVSRLRNFCIIAHIDHGKSTLADRLLQDTGTVAGRDMQEQFLDNMDLERERGITIKLQAARMNYTAADGESYVLNLI
DTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENDLEIIPVLNKIDLPGSDPERIKEEIEAIIGLDTS
TAIACSAKTGLGVSEIMQAVVDRIPPPADTLDEPTRALIFDSYYDSYRGVIVYFRVISGRISTKDKVLLMASKKSYELDE
IGVMSPDQCEVNELHAGEVGYLAASIKAVADARVGDTITLLNAPADEPLPGYTEAKPMVFCGLFPTDADQYPDLREALDK
LQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNMIDGQMLMVDNPATLPDPQKR
ESIEEPYVRMEIYAPNEYNGTLMGLCQERRGEYIDMKYLTTERVTLIYELPLAEVVTDFFDQMKSRTKGYASMEYHLIGY
RRNDLVRLDVLINAEKADPLTTIAHRDKAYSIGKGLVEKLKELIPRQQFKIPLQASIGSRIIASESISAMRKDVLAKCYG
GDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQTS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.9291598023064, Blast_Score=633, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=250, Percent_Identity=36.4, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI18390331, Length=208, Percent_Identity=33.1730769230769, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI25306283, Length=157, Percent_Identity=43.312101910828, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI25306287, Length=157, Percent_Identity=43.312101910828, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI19923640, Length=157, Percent_Identity=43.312101910828, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=42.2018348623853, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=42.2018348623853, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=42.2018348623853, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI217272894, Length=163, Percent_Identity=31.2883435582822, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI217272892, Length=163, Percent_Identity=31.2883435582822, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI94966752, Length=249, Percent_Identity=29.3172690763052, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI53729339, Length=207, Percent_Identity=28.5024154589372, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729337, Length=207, Percent_Identity=28.5024154589372, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI34147630, Length=248, Percent_Identity=28.2258064516129, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=56.2289562289562, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=475, Percent_Identity=31.1578947368421, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789738, Length=203, Percent_Identity=32.0197044334975, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1790835, Length=184, Percent_Identity=33.1521739130435, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790412, Length=286, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789737, Length=286, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.3398692810458, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533571, Length=148, Percent_Identity=41.8918918918919, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=40, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=253, Percent_Identity=28.8537549407115, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=253, Percent_Identity=28.8537549407115, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=37.4193548387097, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.0902255639098, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115532065, Length=274, Percent_Identity=25.1824817518248, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI115532067, Length=274, Percent_Identity=25.1824817518248, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=44.3708609271523, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6323098, Length=185, Percent_Identity=38.3783783783784, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=42.4657534246575, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=42.4657534246575, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=116, Percent_Identity=42.2413793103448, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=39.1608391608392, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322675, Length=148, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=254, Percent_Identity=29.1338582677165, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=607, Percent_Identity=44.6457990115321, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=187, Percent_Identity=35.8288770053476, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=45.2554744525547, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=39.4557823129252, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=32.7160493827161, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550900, Length=289, Percent_Identity=26.9896193771626, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221458488, Length=146, Percent_Identity=39.041095890411, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28572034, Length=146, Percent_Identity=36.3013698630137, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67275; Mature: 67144

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPVSRLRNFCIIAHIDHGKSTLADRLLQDTGTVAGRDMQEQFLDNMDLERERGITIK
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHCCCEEE
LQAARMNYTAADGESYVLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENDLEIIPVLNKIDLPGSDPERIKEEIEAIIGLDTSTAIACSAKTGLGVSEIMQA
EEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
VVDRIPPPADTLDEPTRALIFDSYYDSYRGVIVYFRVISGRISTKDKVLLMASKKSYELD
HHHCCCCCCCCCCCCCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHH
EIGVMSPDQCEVNELHAGEVGYLAASIKAVADARVGDTITLLNAPADEPLPGYTEAKPMV
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPTDADQYPDLREALDKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKVNMIDGQMLMVDNPATLPDPQKRESIEEPYVRMEIYAPNEYN
HHHCCCEEEEECCCEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCC
GTLMGLCQERRGEYIDMKYLTTERVTLIYELPLAEVVTDFFDQMKSRTKGYASMEYHLIG
CHHHHHHHHHCCCEEEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
YRRNDLVRLDVLINAEKADPLTTIAHRDKAYSIGKGLVEKLKELIPRQQFKIPLQASIGS
ECCCCEEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHCCCC
RIIASESISAMRKDVLAKCYGGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NQTS
CCCC
>Mature Secondary Structure 
TDVPVSRLRNFCIIAHIDHGKSTLADRLLQDTGTVAGRDMQEQFLDNMDLERERGITIK
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHCCCEEE
LQAARMNYTAADGESYVLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENDLEIIPVLNKIDLPGSDPERIKEEIEAIIGLDTSTAIACSAKTGLGVSEIMQA
EEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
VVDRIPPPADTLDEPTRALIFDSYYDSYRGVIVYFRVISGRISTKDKVLLMASKKSYELD
HHHCCCCCCCCCCCCCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHH
EIGVMSPDQCEVNELHAGEVGYLAASIKAVADARVGDTITLLNAPADEPLPGYTEAKPMV
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPTDADQYPDLREALDKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKVNMIDGQMLMVDNPATLPDPQKRESIEEPYVRMEIYAPNEYN
HHHCCCEEEEECCCEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCC
GTLMGLCQERRGEYIDMKYLTTERVTLIYELPLAEVVTDFFDQMKSRTKGYASMEYHLIG
CHHHHHHHHHCCCEEEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
YRRNDLVRLDVLINAEKADPLTTIAHRDKAYSIGKGLVEKLKELIPRQQFKIPLQASIGS
ECCCCEEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHCCCC
RIIASESISAMRKDVLAKCYGGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NQTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]