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The map label for this gene is mrcB [H]

Identifier: 124023761

GI number: 124023761

Start: 1818695

End: 1820671

Strand: Direct

Name: mrcB [H]

Synonym: P9303_20671

Alternate gene names: 124023761

Gene position: 1818695-1820671 (Clockwise)

Preceding gene: 124023760

Following gene: 124023764

Centisome position: 67.79

GC content: 55.79

Gene sequence:

>1977_bases
GTGACTCGCAAGCGCCGTCACTGGATTGTTATTGCAGGTAGTGCTATCGCCATCGGGTGTGTCGCAGCCTTGGCTGAAAG
GACTCTCACTGATGCCTTGGATTCGGTTCTGCCCGATGCCCGGCGGATTGCAAACTTCAACCGGCCAGGCACCATCACGC
TGCTCTCCACTGATGGGCAGGTGATCCAAAAGCTTGGTCCCGCGACCCGCGAGAAGGTTGAGCAGGACAAGATGCCTCTA
CTTGTGGAGCAGGCCTTCGTCGCGGCAGAAGACCGCCGCTTTTACTACCACAATGGCTTGGACATCTGGGGTATCAGCCG
AGCCCTGGTGACAAACCTCCGCCAGGGTTCCGTGAGGGAAGGTGCCAGCACCATCACTCAGCAACTTGCTCGAACCGTAT
TCCTGAGTCAGGAACGAACCCTGCCGCGCAAACTCAAAGAAGCTGCTCTGGCCTACAAGCTGGAGCGACAGCTCAGCAAA
CAGCAAATCCTTGAGCAGTATCTGAATTACGTCTACTTGGGCTCAGGTGCTTACGGCGTAGCCGATGCAGCCTGGGTGTA
CTTCTCTAAATCCCCCAATCAACTCACACTGCCGGAAGCCGCTCTAATCGCTGGCATGCCCCCTGCTCCCTCAGTGTATT
CACCACTGGTCAACCAGAAAATCGCCCTAGAACGCCGCGCAATTGTCCTTAAACGAATGCAGCAGGCCGGATTTATCTCT
GCCAAAGAAGCCGACGCAGCTCGCAACAGCCCCCTCAATCTCAAGCCAGCGATCCCGAAGTACTTCAACAGCAGCGCACC
ATTTTTCACCAGCTGGGTAGCCCAGCAACTCCCCAATCTGCTCACCCCAGAGCAACTAGAAGTGGGTGGACTGAAGATTC
ACACCAGCCTCAATCTGGCTTGGCAAAAGCAAGCCCAAGAGGTGATCCGCAAATACGCCCCCGGGAACACAGAGGGTTCC
ATGGTGTCTATCGAACCAAGCAGCGGTGAAGTGCGGGTGATGGTAGGAGGCAAAAACTTCAATACCAGCCAATTCAATCG
AGCAACACAGGCTCTGCGTTCACCTGGCTCCACCTTCAAGCTTTTTCCCTATGCAGCCGCCATCAACGCCGGCATCAAGC
CAGAGGACAAGGTGGTGGACGCGAAACGCTGCTGGAGAGGATATTGCCCAAAGAACTTCGGCAACAAATACCTGGGATCA
GTAGCACTCGCTGATGCCCTGAGATACTCCCTAAACACTGTTGCAGTCCAACTACTAGACAAGGTGGGCTTTGATGCCGT
GATCAGCATGGCCAATAATCTTGGCATCGGCACCACTCGCCCATTGGGCAAGTACTATCCGCTCGCTATTGGGGCCTACG
AGCAAACAGTGCTCGACATGACGGCAGCCTATGCCGCAGTAGCCAACCGGGGCATTTACATGAAGCCCACGCCATTTGAA
GAGATCCGAGGCCCAGACGACGAGGTGATCTGGAACCATCGATTCGAAGGCTACAAAGGTCGTCGAGCCCTCGACAGCGA
CGTAGCAGACACCATGAACTGGATGCTCCAGCGCGTTGTCAGCGGTGGCACTGGAGCTGCGGCATCCCTCGGTGACCGGC
CGGTGGCGGGCAAAACCGGCACATCGGAAGGTGCCCGTGACCTCTGGTTCATTGGCTCAATACCACAACTAACAACCGCC
GTATGGTTTGGCTACGACAACAACCGAAAGACATCTGGCGGCAGTGGTGATGCTGCATGGGCCTGGAAGCAGTTCATGAC
CAAGATCCAAGACAATTTTGAGGTGCAACCCTTCCCACCAAAACCAGTTATGAACCGCACCTTTAGGCCACCAGGCAAAA
CAAGGAGCAAACCAAAGAACGGCAGCGAGGATGCTGCCAAAGACCAAGATCAAACCCAGGGCCCTCCGCCCAGATACATC
GCACCGCCAGGGGGACCACCTCTCAATGAAAACTTCGAGCCAATGCCTATGCCCTGA

Upstream 100 bases:

>100_bases
CCTTTGATGTGAGATATCAGACAAGTGCTCAACCATTCCTAATCCTTGGCATGTTGGTTACTGCTCTAGCCATAGGCCAT
AGCCCATTAACACAGGTGAT

Downstream 100 bases:

>100_bases
TCAGGCTCAGCTGAGATCCAACACGGCTGAATAAAAACCATCACCACCATGCTCAGGATCAGGCCATAGTTGCTGTTCCT
GAGACAAGCTCAATTCAGGA

Product: penicillin binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MTRKRRHWIVIAGSAIAIGCVAALAERTLTDALDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVEQDKMPL
LVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSK
QQILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
AKEADAARNSPLNLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQEVIRKYAPGNTEGS
MVSIEPSSGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGS
VALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
EIRGPDDEVIWNHRFEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTA
VWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYI
APPGGPPLNENFEPMPMP

Sequences:

>Translated_658_residues
MTRKRRHWIVIAGSAIAIGCVAALAERTLTDALDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVEQDKMPL
LVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSK
QQILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
AKEADAARNSPLNLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQEVIRKYAPGNTEGS
MVSIEPSSGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGS
VALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
EIRGPDDEVIWNHRFEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTA
VWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYI
APPGGPPLNENFEPMPMP
>Mature_657_residues
TRKRRHWIVIAGSAIAIGCVAALAERTLTDALDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVEQDKMPLL
VEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQ
QILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFISA
KEADAARNSPLNLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQEVIRKYAPGNTEGSM
VSIEPSSGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSV
ALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFEE
IRGPDDEVIWNHRFEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTAV
WFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIA
PPGGPPLNENFEPMPMP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=532, Percent_Identity=32.8947368421053, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082258, Length=305, Percent_Identity=39.672131147541, Blast_Score=209, Evalue=7e-55,
Organism=Escherichia coli, GI1788867, Length=595, Percent_Identity=29.4117647058824, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=38.2352941176471, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 72308; Mature: 72177

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKRRHWIVIAGSAIAIGCVAALAERTLTDALDSVLPDARRIANFNRPGTITLLSTDGQ
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCHH
VIQKLGPATREKVEQDKMPLLVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVRE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHCCCHHH
GASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQQILEQYLNYVYLGSGAYGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCC
ADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
CCEEEEEEECCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AKEADAARNSPLNLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLA
CHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHH
WQKQAQEVIRKYAPGNTEGSMVSIEPSSGEVRVMVGGKNFNTSQFNRATQALRSPGSTFK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
LFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSVALADALRYSLNTVAVQLLD
EEEHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
HHCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEECCCCHH
EIRGPDDEVIWNHRFEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTG
HHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
TSEGARDLWFIGSIPQLTTAVWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPP
CCCCCCCEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
KPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIAPPGGPPLNENFEPMPMP
CHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRKRRHWIVIAGSAIAIGCVAALAERTLTDALDSVLPDARRIANFNRPGTITLLSTDGQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCHH
VIQKLGPATREKVEQDKMPLLVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVRE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHCCCHHH
GASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQQILEQYLNYVYLGSGAYGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCC
ADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
CCEEEEEEECCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AKEADAARNSPLNLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLA
CHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHH
WQKQAQEVIRKYAPGNTEGSMVSIEPSSGEVRVMVGGKNFNTSQFNRATQALRSPGSTFK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
LFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSVALADALRYSLNTVAVQLLD
EEEHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
HHCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEECCCCHH
EIRGPDDEVIWNHRFEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTG
HHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
TSEGARDLWFIGSIPQLTTAVWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPP
CCCCCCCEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
KPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIAPPGGPPLNENFEPMPMP
CHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]