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The map label for this gene is hisH [H]

Identifier: 124023715

GI number: 124023715

Start: 1774726

End: 1775382

Strand: Direct

Name: hisH [H]

Synonym: P9303_20151

Alternate gene names: 124023715

Gene position: 1774726-1775382 (Clockwise)

Preceding gene: 124023714

Following gene: 124023716

Centisome position: 66.16

GC content: 36.83

Gene sequence:

>657_bases
ATGGCGCCCAAAATTCAAACTGTTATGAAGAGCTATAGTGATACTGTTGTTGTGATTGACTATGGAATGGGAAATATGTG
GTCTGTAATTAGTGCATTAAAGTATTTAAAGATCGAATACATTGTTACAAGCGACCCATGCAAGGTTGAAATAGCTAAGA
TACTTCTGCTGCCTGGGGTGGGATCATTCCGCAAAGGAATTGAATCGCTCATCGATTTGAATCTCATCGATCCTATTATT
GAAGCGGTCACTATTAGAAAGGCAAAGATACTTGGAATCTGTTTGGGGTTTCAGCTGCTTGCGATTTCTAGTACAGAAGA
TGGGTATACAAGCGGTCTCGGTGTAATACCAGGTATCGTAGAAAGATTCTCAGAAGGACAAGTAAACCCTATGAAGGTAC
CTCATATAGGTTTTAGTACTATAAATATCAATAAAAAAGAAGGGCTCTTTGAAGGGTTTGATACAAATGCCGACTTTTAT
TTTGTTCATTCATATAGAATATCACCATTATTAATAGCAGATAATTATGCAACATGTAAGTATGGTGAGAAGTTTCTTGC
GGCCTACCATCATAAAAATGTCATGGGAACACAATTTCATCCTGAAAAAAGTCAAACAAACGGACTGGCACTAATCAACA
ATTTCATGAAGATTTAA

Upstream 100 bases:

>100_bases
CAGAGATGTATCTAGAGTACTTTGAAGTTAATCAAGAAGAGTTTGATCAGATCCTAGACCAATGGGCAAACAAAAAATTA
TTTAACAAGATCAAGGGTAG

Downstream 100 bases:

>100_bases
TCAATGCCTAAAAAACGAATAATATTTACACTTTTGTACGATAGTCAAAGCGAACATTTCTTGTTAAGCAGGAATTTTCG
CGTGCAGGAAGTTGGAGATT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MAPKIQTVMKSYSDTVVVIDYGMGNMWSVISALKYLKIEYIVTSDPCKVEIAKILLLPGVGSFRKGIESLIDLNLIDPII
EAVTIRKAKILGICLGFQLLAISSTEDGYTSGLGVIPGIVERFSEGQVNPMKVPHIGFSTININKKEGLFEGFDTNADFY
FVHSYRISPLLIADNYATCKYGEKFLAAYHHKNVMGTQFHPEKSQTNGLALINNFMKI

Sequences:

>Translated_218_residues
MAPKIQTVMKSYSDTVVVIDYGMGNMWSVISALKYLKIEYIVTSDPCKVEIAKILLLPGVGSFRKGIESLIDLNLIDPII
EAVTIRKAKILGICLGFQLLAISSTEDGYTSGLGVIPGIVERFSEGQVNPMKVPHIGFSTININKKEGLFEGFDTNADFY
FVHSYRISPLLIADNYATCKYGEKFLAAYHHKNVMGTQFHPEKSQTNGLALINNFMKI
>Mature_217_residues
APKIQTVMKSYSDTVVVIDYGMGNMWSVISALKYLKIEYIVTSDPCKVEIAKILLLPGVGSFRKGIESLIDLNLIDPIIE
AVTIRKAKILGICLGFQLLAISSTEDGYTSGLGVIPGIVERFSEGQVNPMKVPHIGFSTININKKEGLFEGFDTNADFYF
VHSYRISPLLIADNYATCKYGEKFLAAYHHKNVMGTQFHPEKSQTNGLALINNFMKI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=206, Percent_Identity=37.378640776699, Blast_Score=124, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=35.6807511737089, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 24163; Mature: 24031

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKIQTVMKSYSDTVVVIDYGMGNMWSVISALKYLKIEYIVTSDPCKVEIAKILLLPGV
CCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCEEEEEEEEEECCC
GSFRKGIESLIDLNLIDPIIEAVTIRKAKILGICLGFQLLAISSTEDGYTSGLGVIPGIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCCHHHHHH
ERFSEGQVNPMKVPHIGFSTININKKEGLFEGFDTNADFYFVHSYRISPLLIADNYATCK
HHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCH
YGEKFLAAYHHKNVMGTQFHPEKSQTNGLALINNFMKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
APKIQTVMKSYSDTVVVIDYGMGNMWSVISALKYLKIEYIVTSDPCKVEIAKILLLPGV
CCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCEEEEEEEEEECCC
GSFRKGIESLIDLNLIDPIIEAVTIRKAKILGICLGFQLLAISSTEDGYTSGLGVIPGIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCCHHHHHH
ERFSEGQVNPMKVPHIGFSTININKKEGLFEGFDTNADFYFVHSYRISPLLIADNYATCK
HHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCH
YGEKFLAAYHHKNVMGTQFHPEKSQTNGLALINNFMKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]