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The map label for this gene is thiE

Identifier: 124023639

GI number: 124023639

Start: 1706747

End: 1707808

Strand: Direct

Name: thiE

Synonym: P9303_19391

Alternate gene names: 124023639

Gene position: 1706747-1707808 (Clockwise)

Preceding gene: 124023637

Following gene: 124023640

Centisome position: 63.62

GC content: 54.9

Gene sequence:

>1062_bases
ATGAAATCGATGCCTGTCGCCCCGATCGCAGATCTACGTGTGGCTCAGCTGATCGATGCCAACCTCGATCGAGCTCGAGA
AGGACTGCGAGTCGTCGAAGACTGGTGCCGCTTCGGCCTAGATCGTGAAGACCTTGTGGTGACTCTCAAAGACTGGCGTC
AGCGACTGGGTCGCCATCATCACGACAGCTACAAACAGGCACGCTCCACTGCTACAGATCAAGGGATCGGCCTCAGTCAT
CCTGCTCAGCAAGAGCGACACGAACCATGGCATGTTGTGGCAGCCAACTGTGCACGCGTTCAAGAAGCTCTACGCGTACT
GGAAGAGTTCGCCCGTCAGCCAGATCCTCAGCTGGCTGCCAGCGCTGCTGCAATCCGCTATGGCCTCTACGACCTAGAGG
TGACCGTGCTGCAGGCCAACGCAGGCAAAAAGAGACGCCAACAACTGCAGGCCTGCCATCTTTGCCTGATTACGACATCA
CAATCCGATCTAGCCAACAACGATCTATTCAGAACAGTGAGCGCAGCACTAGTCGCTGGCATCGACATGGTGCAATACCG
CAATAAAGAAGCTAGCGACTTGCAACGACTGACTCAGGCAAAAGAGCTGGCCAGCCTATGCAGAAAGCATGGGGCGCTAT
TCATCGTTAATGACCGAATCGACTTAGCCCTTGCAGTGGACGCCGATGGCGTTCATCTCGGCCAGGACGACCTCCCCACA
GACGTAGCCAGGGGACTGATCGGCAGCGAACGACTACTGGGTCGAAGCACACAGTTCCTTGCCCAGCTTCAAAAAGCTGA
AGCAGAAGGTTGCGACTATCTAGGAGTAGGGCCTGTCAACAGCACAGCCACAAAACCGGAACGACAACCAATTGGGCTTG
CCTATGTGAAGGAGGCATCTAAAGCCACCCAGCTACCTTGGTTTGCCATTGGTGGCATCAACATCTCAAACCTAGAAGCA
GTACGTCAAGCCGGAGCAAAGCGAATCGCTGTGATCGGAGCGATCATGAATTCCAAAGATCCTGCCGCTACCAGCCTTCA
ACTACTGGAGGCTCTGAGATGA

Upstream 100 bases:

>100_bases
CCACCTAAAAGAACAACCCCCCAGATCAGAACGTTCATGGTGCTATCTGAACCACTGTTGTCCATGATCGGAATGGGGAT
GCAATTGTGCCAAGACTGCC

Downstream 100 bases:

>100_bases
AACTGATGGTGAATGGTGAAATCAGGGACATCGAGCCAGAACCGATGCCAACCTCGCTGGCGGCCGTGATCGAACAGCTG
GGCTACCACCCTCGCCTTGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKSMPVAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREDLVVTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQACHLCLITTS
QSDLANNDLFRTVSAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARGLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
VRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR

Sequences:

>Translated_353_residues
MKSMPVAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREDLVVTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQACHLCLITTS
QSDLANNDLFRTVSAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARGLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
VRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
>Mature_353_residues
MKSMPVAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREDLVVTLKDWRQRLGRHHHDSYKQARSTATDQGIGLSH
PAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAASAAAIRYGLYDLEVTVLQANAGKKRRQQLQACHLCLITTS
QSDLANNDLFRTVSAALVAGIDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
DVARGLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEASKATQLPWFAIGGINISNLEA
VRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=176, Percent_Identity=32.3863636363636, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=222, Percent_Identity=33.7837837837838, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_PROM3 (A2CB21)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017946.1
- ProteinModelPortal:   A2CB21
- SMR:   A2CB21
- STRING:   A2CB21
- GeneID:   4777528
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_19391
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   EEWCRFG
- ProtClustDB:   PRK02615
- HAMAP:   MF_01327
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000512
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 38727; Mature: 38727

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMPVAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREDLVVTLKDWRQRLGRHH
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHHHHCCCC
HDSYKQARSTATDQGIGLSHPAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAA
CHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAAAIRYGLYDLEVTVLQANAGKKRRQQLQACHLCLITTSQSDLANNDLFRTVSAALVAG
HHHHHHHCEEEEEEEEEECCCCHHHHHHHHHHHEEEEECCCHHHCCHHHHHHHHHHHHHH
IDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCHH
DVARGLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEAS
HHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
KATQLPWFAIGGINISNLEAVRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
HHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKSMPVAPIADLRVAQLIDANLDRAREGLRVVEDWCRFGLDREDLVVTLKDWRQRLGRHH
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHHHHCCCC
HDSYKQARSTATDQGIGLSHPAQQERHEPWHVVAANCARVQEALRVLEEFARQPDPQLAA
CHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAAAIRYGLYDLEVTVLQANAGKKRRQQLQACHLCLITTSQSDLANNDLFRTVSAALVAG
HHHHHHHCEEEEEEEEEECCCCHHHHHHHHHHHEEEEECCCHHHCCHHHHHHHHHHHHHH
IDMVQYRNKEASDLQRLTQAKELASLCRKHGALFIVNDRIDLALAVDADGVHLGQDDLPT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCCCHH
DVARGLIGSERLLGRSTQFLAQLQKAEAEGCDYLGVGPVNSTATKPERQPIGLAYVKEAS
HHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
KATQLPWFAIGGINISNLEAVRQAGAKRIAVIGAIMNSKDPAATSLQLLEALR
HHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA