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The map label for this gene is pheS [H]

Identifier: 124023634

GI number: 124023634

Start: 1703140

End: 1704147

Strand: Reverse

Name: pheS [H]

Synonym: P9303_19341

Alternate gene names: 124023634

Gene position: 1704147-1703140 (Counterclockwise)

Preceding gene: 124023636

Following gene: 124023633

Centisome position: 63.52

GC content: 56.85

Gene sequence:

>1008_bases
GTGAGCGCCACTATCTCCTTGCAGCAGCTCACCGACCAGCTTGATGCCCTCGAGGCTGAGGCGGTGTCTGCTATCGCTGC
TGCGGCTGATGCAGATGCCTTGGAGCAATTGCGGGTGAGCCTGTTGGGCAAGAAAGGTCGCTTGTCTGGTGTTCTCGGGG
CGATGGGCAAGCTCCCTAGTGAAGAGCGACCGCTTGTTGGTCAGCGCGCCAATGTGCTTAAAACACAGGTGCAAAACCTG
CTAGGGGAACGGTTGCAGGTGGTGAAAGCGGCAGCTTTGGCTGATCGGCTGCTGCGGGAAACGATTGATGTCACTGCGCC
TTCCAGTGGCATTCCTGTGGGGCATCGCCACCCTTTGATTACAACAACTGAGCAGATCGTGGATCTGTTTTGTGGTTTGG
GCTATCGGGTGGCTGAAGGCCCGGAGGTAGAGACCGATCACTACAACTTTTCCGCCTTGAATATTCCCGAGGACCATCCC
GCTCGAGATATGCAGGACACGTTCTATCTGGGCGGCAATCTGCTCCTGCGTACCCACACCTCGCCTGTGCAGATTCGTTA
TCTAGAAGAGAATCCGCCGCCGGTAAGGATTGTGGCGCCAGGCCGGGTGTATCGGCGTGATGCAGTGGATGCGACCCATT
CGCCTGTCTTTCATCAGGTTGAGGTGTTGGCCATTGATGAAGGCCTTGATTTCAGCCATCTGCGCGGCACGGTGATGGCC
TTCCTGAAGGCATTCTTTGGCGATATGCCGGTGAGATTTCGGGCTAGTTATTTCCCCTTTACCGAACCATCGGCTGAGGT
GGATGTGCAGTGGCGTGGGCGTTGGCTGGAGGTGATGGGTTGCGGCATGGTTGATCCAGCAGTCTTAGAGGGCCTGGGGC
TTGATCCAGAGCGCTGGAGTGGGTTTGCGGCAGGCCTTGGTGTGGAACGCTTCTGCATGGTGCGTCATGGCATCGACGAC
ATTCGCAGGCTTTATACGAGTGATCTGCGCTTCCTTGAGCAGTTTTGA

Upstream 100 bases:

>100_bases
CAACGGAGTCATCTCCTTGTATCCACTGCGGGGAACTTAATCATGATGACTGCCTAAAGCATGATGACTGCCTAAAGTCA
GTGGCTGCGATCTTTTTTCA

Downstream 100 bases:

>100_bases
GTCGATAGCCGATCGTGCACTGGATGAAGCATCGCCGCGTTGCTGCATCCAAAAACAGCTGAATCCCCATAAAGTGAGGT
CGTTGTTTGCTTCATTCGAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNL
LGERLQVVKAAALADRLLRETIDVTAPSSGIPVGHRHPLITTTEQIVDLFCGLGYRVAEGPEVETDHYNFSALNIPEDHP
ARDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDD
IRRLYTSDLRFLEQF

Sequences:

>Translated_335_residues
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNL
LGERLQVVKAAALADRLLRETIDVTAPSSGIPVGHRHPLITTTEQIVDLFCGLGYRVAEGPEVETDHYNFSALNIPEDHP
ARDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDD
IRRLYTSDLRFLEQF
>Mature_334_residues
SATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPSEERPLVGQRANVLKTQVQNLL
GERLQVVKAAALADRLLRETIDVTAPSSGIPVGHRHPLITTTEQIVDLFCGLGYRVAEGPEVETDHYNFSALNIPEDHPA
RDMQDTFYLGGNLLLRTHTSPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMAF
LKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWSGFAAGLGVERFCMVRHGIDDI
RRLYTSDLRFLEQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=260, Percent_Identity=34.6153846153846, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI5729820, Length=230, Percent_Identity=34.3478260869565, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=51.6923076923077, Blast_Score=329, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI32566635, Length=301, Percent_Identity=32.890365448505, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=32.3420074349442, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=32.3420074349442, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=276, Percent_Identity=32.9710144927536, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=88, Percent_Identity=44.3181818181818, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=295, Percent_Identity=30.1694915254237, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137424, Length=278, Percent_Identity=29.8561151079137, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37016; Mature: 36885

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPS
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
EERPLVGQRANVLKTQVQNLLGERLQVVKAAALADRLLRETIDVTAPSSGIPVGHRHPLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
TTTEQIVDLFCGLGYRVAEGPEVETDHYNFSALNIPEDHPARDMQDTFYLGGNLLLRTHT
ECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCHHEEECCEEEEEECC
SPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
CCEEEEEECCCCCCEEEECCCHHHHHHCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHH
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWS
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHHHHC
GFAAGLGVERFCMVRHGIDDIRRLYTSDLRFLEQF
CHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATISLQQLTDQLDALEAEAVSAIAAAADADALEQLRVSLLGKKGRLSGVLGAMGKLPS
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
EERPLVGQRANVLKTQVQNLLGERLQVVKAAALADRLLRETIDVTAPSSGIPVGHRHPLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
TTTEQIVDLFCGLGYRVAEGPEVETDHYNFSALNIPEDHPARDMQDTFYLGGNLLLRTHT
ECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCHHEEECCEEEEEECC
SPVQIRYLEENPPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMA
CCEEEEEECCCCCCEEEECCCHHHHHHCCCCCCCCHHHEEEEEEECCCCCHHHHHHHHHH
FLKAFFGDMPVRFRASYFPFTEPSAEVDVQWRGRWLEVMGCGMVDPAVLEGLGLDPERWS
HHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHCCCCCHHHHC
GFAAGLGVERFCMVRHGIDDIRRLYTSDLRFLEQF
CHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]