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The map label for this gene is def [H]

Identifier: 124023604

GI number: 124023604

Start: 1664947

End: 1665492

Strand: Direct

Name: def [H]

Synonym: P9303_19041

Alternate gene names: 124023604

Gene position: 1664947-1665492 (Clockwise)

Preceding gene: 124023603

Following gene: 124023607

Centisome position: 62.06

GC content: 50.73

Gene sequence:

>546_bases
ATGGCTGTGAAGGAAATCCTGCGTATGGGCAACCCTCAATTGAGGCAAGTGTCCAAGGTCGTCGATGATGTCTCTGCTGA
GCTGATCATTTCACTCATCAAGGATTTACAAGACACGGTGAAGGCCCATCAAGGAGCAGGACTCGCCGCACCCCAAATTG
GTATCCCTTTAAGAGTGGTGCTGTTTGGTGGAGGAGGCCCTAATCCCCGCTATCCCGAGGCCCCATCGATTCCACAAACA
CTTCTGATTAATCCTGTCTTAACACCAATCGACTCTGATCTAGAGGATGGTTGGGAGGGGTGCTTGAGTGTGCCAGGCCT
GCGGGGCAAGGTATCCCGCTGGAGTCGGATTCACTACAGGGCGCTCAATGAAGATGGTTTAGAGATTGAGCATTGCCTTG
AGGGATTCCCAGCAAGAGTGATCCAACATGAATGTGACCACTTAGACGGGGTTTTGTTTACCGATCGTCTAGTTGACTCT
GCCTCATTTGGATTCACAGGGGAGTTAGAAGCTGCTGGAGTGGTCCCTATGAAAACAGATAAGTAG

Upstream 100 bases:

>100_bases
TGATAACTCACTTGTCCATTGACAGAATCTTGCTCATCCTTGTGGCTGGTTTTGGTGTGTTGGCGAGAACTACAGCAAAG
AGAAAAGGAGGTTAATAATC

Downstream 100 bases:

>100_bases
TAGATAAGTATAAGCAAACTCTGCAAAAAAGAGATTACGAATTAATTAGAAAAAATAGATTTGCATTAAAAAAAGCAATA
AAAGCAATGAAGAAAAACAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MAVKEILRMGNPQLRQVSKVVDDVSAELIISLIKDLQDTVKAHQGAGLAAPQIGIPLRVVLFGGGGPNPRYPEAPSIPQT
LLINPVLTPIDSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGLEIEHCLEGFPARVIQHECDHLDGVLFTDRLVDS
ASFGFTGELEAAGVVPMKTDK

Sequences:

>Translated_181_residues
MAVKEILRMGNPQLRQVSKVVDDVSAELIISLIKDLQDTVKAHQGAGLAAPQIGIPLRVVLFGGGGPNPRYPEAPSIPQT
LLINPVLTPIDSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGLEIEHCLEGFPARVIQHECDHLDGVLFTDRLVDS
ASFGFTGELEAAGVVPMKTDK
>Mature_180_residues
AVKEILRMGNPQLRQVSKVVDDVSAELIISLIKDLQDTVKAHQGAGLAAPQIGIPLRVVLFGGGGPNPRYPEAPSIPQTL
LINPVLTPIDSDLEDGWEGCLSVPGLRGKVSRWSRIHYRALNEDGLEIEHCLEGFPARVIQHECDHLDGVLFTDRLVDSA
SFGFTGELEAAGVVPMKTDK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1789682, Length=158, Percent_Identity=37.3417721518987, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=33.1360946745562, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19640; Mature: 19509

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKEILRMGNPQLRQVSKVVDDVSAELIISLIKDLQDTVKAHQGAGLAAPQIGIPLRVV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LFGGGGPNPRYPEAPSIPQTLLINPVLTPIDSDLEDGWEGCLSVPGLRGKVSRWSRIHYR
EECCCCCCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALNEDGLEIEHCLEGFPARVIQHECDHLDGVLFTDRLVDSASFGFTGELEAAGVVPMKTD
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
K
C
>Mature Secondary Structure 
AVKEILRMGNPQLRQVSKVVDDVSAELIISLIKDLQDTVKAHQGAGLAAPQIGIPLRVV
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LFGGGGPNPRYPEAPSIPQTLLINPVLTPIDSDLEDGWEGCLSVPGLRGKVSRWSRIHYR
EECCCCCCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ALNEDGLEIEHCLEGFPARVIQHECDHLDGVLFTDRLVDSASFGFTGELEAAGVVPMKTD
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]