Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 124023593

GI number: 124023593

Start: 1654972

End: 1656162

Strand: Direct

Name: amiC [H]

Synonym: P9303_18931

Alternate gene names: 124023593

Gene position: 1654972-1656162 (Clockwise)

Preceding gene: 124023592

Following gene: 124023594

Centisome position: 61.69

GC content: 56.51

Gene sequence:

>1191_bases
GTGCAGCCATTGCTCCTGTGGATAACTCGCACATTGGTCGCATTCGCGCGCAAATGCCAAGCCTCCAACACCGGCAGGCA
GCACTGTTCTGAGCCAATGGCCTCGGCTCCGTCCCGCCCTCTCATCCTGCTGGTGGTTGGTGCCTTGCAGATCCCTTTGG
TTTTGGCAGCGTTGCCGGCCAGAGCGGCAAGCGCTTTAGCGGCCTGGGCACTTGGAAGCGACGGGGTGTTGCAGTTGCGT
ACATCAAGAGGGGCTCGTCTAGAAGCGTTCTTTCAACCAGCGGATGGTGCCCAAGGAGCAAAGGTCTGGATTGATTTTCC
TGGAGAATTGAGCCGCTCCCGCAGCCTGCGGGGTAGCGGTCCGGTACGTGCGATTCGTTTGGGGAAACCAACGCCTGGAT
CAACTCGACTGGTGATTGAGTTCAAACCATCAATCTCTCTGAATCCAAATCAGCTCAAGCTGATAGGAACCTCTCCGGAC
CGCTGGAAGCTCAACTTCGAAGGCTTACCGACCCGTGGGCTTCGCCGTATCGGTGAAGGAGACCTGACAGCATCAACTTT
GAGCCGATGGGCTCCTGGGATCCGGATTACCCCCACACGGACGCCTATCAATGCCTCTGGTTTGCCGAATGTCCCGCGCG
GGCGCTTTAGAGTTGTCATTGACCCTGGTCATGGTGGTCCAGACCCTGGTGCTATAGGGATTCGCGGTGTGCGTGAGACG
GATGTTGTTCTCGATGTTTCCCTTCAGGTGGCCCAGCTATTAGAAGCTCGAGGGGTGCAGGTCATCATGACCCGCACGGC
TGATGTGGATGTGGATCTACCGCCAAGAGTGGCGATCGCCAATCGAGTCGCTGCTACTGCATTTGTAAGCATTCATGCCA
ATGCCATCAGCATGTCCAGACCACAGGTCAATGGAATCGAAACCTTCTATTTCTCTGATTCCCGTTCAGCCAGGTTGGCG
TCTCATATCCAGCAACAGGTGCTGAACGTCTCCCCTGGAAGCCCTAACAGAGGGGTCAGACGGGGCCGTTTCTTTGTCAT
CCGGCGAACCACAATGCCCTCCGCCCTTGTTGAGACAGGTTTTTTAACAGGACGGCTAGATGCACCTCGGCTGGCTTCAG
CATCCCATCGCCGCAAGTTGGCTCTGGCTATTGCTACTGGCATCCTCAACTATTTGCAGGGGGTCCGTTGA

Upstream 100 bases:

>100_bases
GCCCAGACAGGTCGTCATTACAGCCGTCGACAGAGCCATGGACATGCCATGGTGATTGATCCCTGGGGAACCGTCTTGGC
TGATGCAGGGGTGAGTCAGG

Downstream 100 bases:

>100_bases
AGCCTCGCTTGGGATTATTTGATAGCGGCCTGGGTGGGCTGACTGTGCTCAGGCGAGTGCTGGAGCGCCATGGGCAGGTG
TCTTGCCTCTATCTCGGTGA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MQPLLLWITRTLVAFARKCQASNTGRQHCSEPMASAPSRPLILLVVGALQIPLVLAALPARAASALAAWALGSDGVLQLR
TSRGARLEAFFQPADGAQGAKVWIDFPGELSRSRSLRGSGPVRAIRLGKPTPGSTRLVIEFKPSISLNPNQLKLIGTSPD
RWKLNFEGLPTRGLRRIGEGDLTASTLSRWAPGIRITPTRTPINASGLPNVPRGRFRVVIDPGHGGPDPGAIGIRGVRET
DVVLDVSLQVAQLLEARGVQVIMTRTADVDVDLPPRVAIANRVAATAFVSIHANAISMSRPQVNGIETFYFSDSRSARLA
SHIQQQVLNVSPGSPNRGVRRGRFFVIRRTTMPSALVETGFLTGRLDAPRLASASHRRKLALAIATGILNYLQGVR

Sequences:

>Translated_396_residues
MQPLLLWITRTLVAFARKCQASNTGRQHCSEPMASAPSRPLILLVVGALQIPLVLAALPARAASALAAWALGSDGVLQLR
TSRGARLEAFFQPADGAQGAKVWIDFPGELSRSRSLRGSGPVRAIRLGKPTPGSTRLVIEFKPSISLNPNQLKLIGTSPD
RWKLNFEGLPTRGLRRIGEGDLTASTLSRWAPGIRITPTRTPINASGLPNVPRGRFRVVIDPGHGGPDPGAIGIRGVRET
DVVLDVSLQVAQLLEARGVQVIMTRTADVDVDLPPRVAIANRVAATAFVSIHANAISMSRPQVNGIETFYFSDSRSARLA
SHIQQQVLNVSPGSPNRGVRRGRFFVIRRTTMPSALVETGFLTGRLDAPRLASASHRRKLALAIATGILNYLQGVR
>Mature_396_residues
MQPLLLWITRTLVAFARKCQASNTGRQHCSEPMASAPSRPLILLVVGALQIPLVLAALPARAASALAAWALGSDGVLQLR
TSRGARLEAFFQPADGAQGAKVWIDFPGELSRSRSLRGSGPVRAIRLGKPTPGSTRLVIEFKPSISLNPNQLKLIGTSPD
RWKLNFEGLPTRGLRRIGEGDLTASTLSRWAPGIRITPTRTPINASGLPNVPRGRFRVVIDPGHGGPDPGAIGIRGVRET
DVVLDVSLQVAQLLEARGVQVIMTRTADVDVDLPPRVAIANRVAATAFVSIHANAISMSRPQVNGIETFYFSDSRSARLA
SHIQQQVLNVSPGSPNRGVRRGRFFVIRRTTMPSALVETGFLTGRLDAPRLASASHRRKLALAIATGILNYLQGVR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=266, Percent_Identity=29.6992481203008, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87082163, Length=336, Percent_Identity=25.8928571428571, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42560; Mature: 42560

Theoretical pI: Translated: 12.23; Mature: 12.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLLLWITRTLVAFARKCQASNTGRQHCSEPMASAPSRPLILLVVGALQIPLVLAALPA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHH
RAASALAAWALGSDGVLQLRTSRGARLEAFFQPADGAQGAKVWIDFPGELSRSRSLRGSG
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCHHHCCCCCCCC
PVRAIRLGKPTPGSTRLVIEFKPSISLNPNQLKLIGTSPDRWKLNFEGLPTRGLRRIGEG
CEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHCCCC
DLTASTLSRWAPGIRITPTRTPINASGLPNVPRGRFRVVIDPGHGGPDPGAIGIRGVRET
CCCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCC
DVVLDVSLQVAQLLEARGVQVIMTRTADVDVDLPPRVAIANRVAATAFVSIHANAISMSR
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHEEEEEEEEECEEECCC
PQVNGIETFYFSDSRSARLASHIQQQVLNVSPGSPNRGVRRGRFFVIRRTTMPSALVETG
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHC
FLTGRLDAPRLASASHRRKLALAIATGILNYLQGVR
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPLLLWITRTLVAFARKCQASNTGRQHCSEPMASAPSRPLILLVVGALQIPLVLAALPA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHH
RAASALAAWALGSDGVLQLRTSRGARLEAFFQPADGAQGAKVWIDFPGELSRSRSLRGSG
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCHHHCCCCCCCC
PVRAIRLGKPTPGSTRLVIEFKPSISLNPNQLKLIGTSPDRWKLNFEGLPTRGLRRIGEG
CEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHCCCC
DLTASTLSRWAPGIRITPTRTPINASGLPNVPRGRFRVVIDPGHGGPDPGAIGIRGVRET
CCCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCC
DVVLDVSLQVAQLLEARGVQVIMTRTADVDVDLPPRVAIANRVAATAFVSIHANAISMSR
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHEEEEEEEEECEEECCC
PQVNGIETFYFSDSRSARLASHIQQQVLNVSPGSPNRGVRRGRFFVIRRTTMPSALVETG
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHC
FLTGRLDAPRLASASHRRKLALAIATGILNYLQGVR
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]