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The map label for this gene is cocE [H]

Identifier: 124023539

GI number: 124023539

Start: 1598749

End: 1600392

Strand: Reverse

Name: cocE [H]

Synonym: P9303_18391

Alternate gene names: 124023539

Gene position: 1600392-1598749 (Counterclockwise)

Preceding gene: 124023540

Following gene: 124023538

Centisome position: 59.66

GC content: 56.45

Gene sequence:

>1644_bases
ATGTGCGTTGAAGGTCGAACGAGCTCTGGATCCGTGAACTGGCACGATGCCTGGCTGACACTCTCTGACGGGGTCAAGCT
CGTTGCCAGGTTATGGGTCCCGAAGGGTGAGGGACCCTGGCCTGCTCTCGTGATGCGTCAGCCCTACGGACGTGCGCTCG
CCTCAACGGTGACTTACATCCATCCTGGTTGGTGGGCAAGTCACGGCTATCTGGTCGTGGTCCAGGACGTACGTGGTCAA
GGGGATTCCGAAGGCCACTTCAATGGCTTCCTGCAAGAAGCTTCTGATACCAGTCAGACGCATGCATGGGTTCGGGAGTT
GCCAGAATGCAATGGCCGTCTTGGAACCTATGGGTTTTCCTATCAGGGCCTAACACAGCTGCTTGCCGAACCCGGGACGC
CGCCACCGGACTGTCTGGCACCAGCGATGGCAGGAGTTGATGAGCGCAACCATTGGAGTTGTGAGGGAGGTGCTCACTGG
TGGCATCTTGGCTTGGCCTGGGGGCTGCAACTTGCAGCACTACAAGCTCGTCGCTGTGGCAACTGGGAAGCATGGAGAGA
GCTTCGCCGCAGTTTGGAAGACGACAGCTACCTGTATGAGGGTCCGGCACTTCTGAAACGCCACGATCCCGATGGAATGA
GCTTGAGATGGCTACAACAAGCGAGCCAAAACGATCAAGGCTGGGTTGTACACAAGCCCTTGGATTCCTGGCTGCGTCAA
CCGATGCTGCTTCTGGGTGGCTGGTGGGACCCCCATTTGAATGGCTTGCTTGATCTCTATCAACGATCAAGCCAAGTAGG
TGGTAGTCCAGAACTTCACATCGGTCCAGCGACTCACCTGCAGTGGTGGCCTGATGCACAGCAACTTCAGCTGGAGTTCT
TTGATCGCCATCTGCAATCTTCGAAAGCCTTAACGAATTCAAGACCCCATGGGCGGATCTGGAATATCACGTCTTGTTCT
TGGCAGAGATTTGTAAGCCCCACCCAGACCACAACATCAGCCCATGCCGGCTGGAGTCTTGTCAGTGGAGGGATGGCCTG
CTTGGACCCCTCAGAAGGCGCCCTGCATCAGAACAAGGAAGGTGGCGGCGTGGTTTATGTGGTCCATGACCCTTGGCGAC
CGGTTCAAGCAGTGGGAGGACATCTCAGCCCAAAACCAGGAGTTGCTGAGCGCAGCGCCGTGGACCAGCGCGCCGATGTG
GCTACCTTCACAAGCACTGCTTTGCAGGAACCTCTCCAACTCAATGGGATCCCATTACTGCAGCTGACCGTGCAGTCAGA
TCAACCGGGATTTGACCTTTGCGTTGCCTTCTCCATTGTTAATCGCAGCCACAGCGAGGTGAAGCAGCTCTCAACAGGTT
TTCTGCGTGTGCAAGGAGAGCAGGCCCTGCGCATGCTGCCGCGCAAGGTGAAACTTCAACCAATATTTGCAGACCTGCAG
CGAGGAGAACATCTGCGCCTATCTCTCGCAGGCGCTGCCTGGCCGGCCATTGGTGTCAACCCAGGCCACGATCGTCATCC
CTGTGGCCCTCCAGGACCCCATTGCCAAGTGGTGACCATGACACTGCAGCTCAATGGATCCAAGTTGAGGCTTTTGCCAT
GGAACTCCGGCAAAATAGATTTCGATTTGCCCCAAGAGTTTTGA

Upstream 100 bases:

>100_bases
TATTCATCTCAAGCGATCTCAGAAGTCGCCCGCCAACGCAATCTCAGCTCCAATAACGCTGAGATTCTGGCCACCTATGT
GATTGGAATGAACTGCCCGG

Downstream 100 bases:

>100_bases
AGCTGTCTGTTTGCCTGCTGAGCCTATCCCTTGGATTGCCCATGGGCCTGAGTGCACTAACCCTTCCGACTGTGGCAGCG
CAGGAGACCATTCAAAAGAC

Product: acyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MCVEGRTSSGSVNWHDAWLTLSDGVKLVARLWVPKGEGPWPALVMRQPYGRALASTVTYIHPGWWASHGYLVVVQDVRGQ
GDSEGHFNGFLQEASDTSQTHAWVRELPECNGRLGTYGFSYQGLTQLLAEPGTPPPDCLAPAMAGVDERNHWSCEGGAHW
WHLGLAWGLQLAALQARRCGNWEAWRELRRSLEDDSYLYEGPALLKRHDPDGMSLRWLQQASQNDQGWVVHKPLDSWLRQ
PMLLLGGWWDPHLNGLLDLYQRSSQVGGSPELHIGPATHLQWWPDAQQLQLEFFDRHLQSSKALTNSRPHGRIWNITSCS
WQRFVSPTQTTTSAHAGWSLVSGGMACLDPSEGALHQNKEGGGVVYVVHDPWRPVQAVGGHLSPKPGVAERSAVDQRADV
ATFTSTALQEPLQLNGIPLLQLTVQSDQPGFDLCVAFSIVNRSHSEVKQLSTGFLRVQGEQALRMLPRKVKLQPIFADLQ
RGEHLRLSLAGAAWPAIGVNPGHDRHPCGPPGPHCQVVTMTLQLNGSKLRLLPWNSGKIDFDLPQEF

Sequences:

>Translated_547_residues
MCVEGRTSSGSVNWHDAWLTLSDGVKLVARLWVPKGEGPWPALVMRQPYGRALASTVTYIHPGWWASHGYLVVVQDVRGQ
GDSEGHFNGFLQEASDTSQTHAWVRELPECNGRLGTYGFSYQGLTQLLAEPGTPPPDCLAPAMAGVDERNHWSCEGGAHW
WHLGLAWGLQLAALQARRCGNWEAWRELRRSLEDDSYLYEGPALLKRHDPDGMSLRWLQQASQNDQGWVVHKPLDSWLRQ
PMLLLGGWWDPHLNGLLDLYQRSSQVGGSPELHIGPATHLQWWPDAQQLQLEFFDRHLQSSKALTNSRPHGRIWNITSCS
WQRFVSPTQTTTSAHAGWSLVSGGMACLDPSEGALHQNKEGGGVVYVVHDPWRPVQAVGGHLSPKPGVAERSAVDQRADV
ATFTSTALQEPLQLNGIPLLQLTVQSDQPGFDLCVAFSIVNRSHSEVKQLSTGFLRVQGEQALRMLPRKVKLQPIFADLQ
RGEHLRLSLAGAAWPAIGVNPGHDRHPCGPPGPHCQVVTMTLQLNGSKLRLLPWNSGKIDFDLPQEF
>Mature_547_residues
MCVEGRTSSGSVNWHDAWLTLSDGVKLVARLWVPKGEGPWPALVMRQPYGRALASTVTYIHPGWWASHGYLVVVQDVRGQ
GDSEGHFNGFLQEASDTSQTHAWVRELPECNGRLGTYGFSYQGLTQLLAEPGTPPPDCLAPAMAGVDERNHWSCEGGAHW
WHLGLAWGLQLAALQARRCGNWEAWRELRRSLEDDSYLYEGPALLKRHDPDGMSLRWLQQASQNDQGWVVHKPLDSWLRQ
PMLLLGGWWDPHLNGLLDLYQRSSQVGGSPELHIGPATHLQWWPDAQQLQLEFFDRHLQSSKALTNSRPHGRIWNITSCS
WQRFVSPTQTTTSAHAGWSLVSGGMACLDPSEGALHQNKEGGGVVYVVHDPWRPVQAVGGHLSPKPGVAERSAVDQRADV
ATFTSTALQEPLQLNGIPLLQLTVQSDQPGFDLCVAFSIVNRSHSEVKQLSTGFLRVQGEQALRMLPRKVKLQPIFADLQ
RGEHLRLSLAGAAWPAIGVNPGHDRHPCGPPGPHCQVVTMTLQLNGSKLRLLPWNSGKIDFDLPQEF

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 60682; Mature: 60682

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVEGRTSSGSVNWHDAWLTLSDGVKLVARLWVPKGEGPWPALVMRQPYGRALASTVTYI
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHEEE
HPGWWASHGYLVVVQDVRGQGDSEGHFNGFLQEASDTSQTHAWVRELPECNGRLGTYGFS
CCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
YQGLTQLLAEPGTPPPDCLAPAMAGVDERNHWSCEGGAHWWHLGLAWGLQLAALQARRCG
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCC
NWEAWRELRRSLEDDSYLYEGPALLKRHDPDGMSLRWLQQASQNDQGWVVHKPLDSWLRQ
CHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHCC
PMLLLGGWWDPHLNGLLDLYQRSSQVGGSPELHIGPATHLQWWPDAQQLQLEFFDRHLQS
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHH
SKALTNSRPHGRIWNITSCSWQRFVSPTQTTTSAHAGWSLVSGGMACLDPSEGALHQNKE
HHHHCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCC
GGGVVYVVHDPWRPVQAVGGHLSPKPGVAERSAVDQRADVATFTSTALQEPLQLNGIPLL
CCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
QLTVQSDQPGFDLCVAFSIVNRSHSEVKQLSTGFLRVQGEQALRMLPRKVKLQPIFADLQ
EEEEECCCCCHHHEEEHHHHCCCHHHHHHHHCCEEEECCHHHHHHCCHHEEECHHHHHHC
RGEHLRLSLAGAAWPAIGVNPGHDRHPCGPPGPHCQVVTMTLQLNGSKLRLLPWNSGKID
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEE
FDLPQEF
CCCCCCC
>Mature Secondary Structure
MCVEGRTSSGSVNWHDAWLTLSDGVKLVARLWVPKGEGPWPALVMRQPYGRALASTVTYI
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHEEE
HPGWWASHGYLVVVQDVRGQGDSEGHFNGFLQEASDTSQTHAWVRELPECNGRLGTYGFS
CCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
YQGLTQLLAEPGTPPPDCLAPAMAGVDERNHWSCEGGAHWWHLGLAWGLQLAALQARRCG
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCC
NWEAWRELRRSLEDDSYLYEGPALLKRHDPDGMSLRWLQQASQNDQGWVVHKPLDSWLRQ
CHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHCC
PMLLLGGWWDPHLNGLLDLYQRSSQVGGSPELHIGPATHLQWWPDAQQLQLEFFDRHLQS
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHH
SKALTNSRPHGRIWNITSCSWQRFVSPTQTTTSAHAGWSLVSGGMACLDPSEGALHQNKE
HHHHCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCC
GGGVVYVVHDPWRPVQAVGGHLSPKPGVAERSAVDQRADVATFTSTALQEPLQLNGIPLL
CCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
QLTVQSDQPGFDLCVAFSIVNRSHSEVKQLSTGFLRVQGEQALRMLPRKVKLQPIFADLQ
EEEEECCCCCHHHEEEHHHHCCCHHHHHHHHCCEEEECCHHHHHHCCHHEEECHHHHHHC
RGEHLRLSLAGAAWPAIGVNPGHDRHPCGPPGPHCQVVTMTLQLNGSKLRLLPWNSGKID
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEE
FDLPQEF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]