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The map label for this gene is rpoD4 [H]

Identifier: 124023525

GI number: 124023525

Start: 1585070

End: 1585996

Strand: Reverse

Name: rpoD4 [H]

Synonym: P9303_18251

Alternate gene names: 124023525

Gene position: 1585996-1585070 (Counterclockwise)

Preceding gene: 124023526

Following gene: 124023524

Centisome position: 59.12

GC content: 50.92

Gene sequence:

>927_bases
ATGGTGAGTTCTTTAAGTGCCTATCTTGGGGAGATCGGGCGCCACCAACTTCTGACACCTGAACAGGAACTCACAATGGG
TCGGAAGGTTCAGGCCATGGTTGCGCTTACTGACCGCTGCCATCTTGCTGGTGGAGCAGGGCCCGAATGCGAATACTCAG
ATGATCAGCGCCTCACCATTCGTCGTGGTGAAAAAGCCAAGAATCAGATGATCACTGCCAATTTAAGACTTGTAGTGAAT
CTTGCTAAGCGATACCAGGGCAAGGGTCTTGATCTTCTCGATCTGATTCAAGAAGGCACGCTTGGACTTACCAGAGCAGT
GGAGAAATATGACCCAACCCGTGGCCATAGATTTTCGACCTATGCCTATTGGTGGATTCGCCAGGGGCTTAACCGGGCAC
TATCCACCCAGAGCAGGACGATACGAATTCCCGTCAATGTGAATGAGAAGCTAACCAAATTAAGGGCAGCAAAGTCAAGA
CTGATGCAGAGCAACGGCTTGCCTCCCACTGCTGAGCAATTGGTAAAAACCATGCGACTGCCGATGGCCGAAGTGGAAGA
TCTGCTCGCTTGTGAATTACGCAGTGTGACAGTGAGCCTTCAAGGAGTTGTGAAGTCAAAATCCGACCCTTCAGAACTTG
TGGATGTCCTTCCCAGCGAAGAGATCCCTCCGATGGAGCGTGCCGAAATGGCGGAAAGGACTGCATCGGTATGGACTTTG
CTCAATCGTGCGAACCTCACTCCTAAAGAACGGATGGTGGTCACACTTCGTTTTGGACTCGACGGATCTCATGAATGGCG
CACCCTCGCAGAAGTTGCACGACATATGAGTTGCAGTCGCGAATACTGCAGGCAGGTGGTACAAAGGGCACTGCGAAAAC
TTCGCAAAACAGGAATTCAGAGTGGTTTGGTAGAAAGCACCCTCTAA

Upstream 100 bases:

>100_bases
AACTGTTTAATAACGCATAAAAAGTTAATTCCGTACTCTTCCTTACGCCGCCAGGATGGAAACACTTTGGCTCTAGGTTC
AATTAAGAGTCGAGTTCCAG

Downstream 100 bases:

>100_bases
GCGATAAACCTCACTGAAAAGTAGGAAATCCTCCCCAAGAAGGGGCGGAGCTCATCTTATGAATCCCCATAAATCAGGTA
AAAATGGAATGACATCGGCG

Product: type II alternative sigma factor, sigma70 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTDRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQSNGLPPTAEQLVKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELVDVLPSEEIPPMERAEMAERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVVQRALRKLRKTGIQSGLVESTL

Sequences:

>Translated_308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTDRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQSNGLPPTAEQLVKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELVDVLPSEEIPPMERAEMAERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVVQRALRKLRKTGIQSGLVESTL
>Mature_308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTDRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQSNGLPPTAEQLVKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELVDVLPSEEIPPMERAEMAERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVVQRALRKLRKTGIQSGLVESTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=38.7234042553192, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=37.3333333333333, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=28.1512605042017, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34683; Mature: 34683

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTDRCHLAGGAGPECEYSDDQRLTI
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
RRGEKAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFST
ECCCHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHH
YAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSRLMQSNGLPPTAEQLVKTMRL
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
PMAEVEDLLACELRSVTVSLQGVVKSKSDPSELVDVLPSEEIPPMERAEMAERTASVWTL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVVQRALRKLRKTGIQ
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVESTL
HCHHHHCC
>Mature Secondary Structure
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTDRCHLAGGAGPECEYSDDQRLTI
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
RRGEKAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFST
ECCCHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHH
YAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSRLMQSNGLPPTAEQLVKTMRL
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
PMAEVEDLLACELRSVTVSLQGVVKSKSDPSELVDVLPSEEIPPMERAEMAERTASVWTL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVVQRALRKLRKTGIQ
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVESTL
HCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA