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The map label for this gene is ecm27 [H]

Identifier: 124023511

GI number: 124023511

Start: 1577077

End: 1578186

Strand: Reverse

Name: ecm27 [H]

Synonym: P9303_18111

Alternate gene names: 124023511

Gene position: 1578186-1577077 (Counterclockwise)

Preceding gene: 124023512

Following gene: 124023508

Centisome position: 58.83

GC content: 53.24

Gene sequence:

>1110_bases
ATGCCGCAATTCCTTGAACCGGCTTTACAAGTGCTGGCAGGAATCGGCCTGTTGTTTGGGGGCGGAGAACTGTTTGTGCA
GGGTTCCGTCGCCCTTGCACTGATCTTCGGTATACCTGAACTTGTGATCGGTCTCACTGTGGTGGCCTTGGGCACATGTG
CCCCGGAGCTGTTCGTCAGTGTGAGTTCAGTGCTCAAGGGCTCCGATGCCCTGGCCGTGAGCAATGTGGTTGGTAGCAAT
ATTTTTAATGTGATGGTGGTGCTCGGCAGCAGTGCCCTTGTGCTGCCACTCAGGGTGGAGAGTCGTTTAGTGCGACGTGA
TGTTCCTCTGCTACTAGCGGTGTCTGCAGCCGTATGGGGAATGGCTTCAGCAGGTCGTGTGACATGGCAGGCCGGAGTAG
CCCTCTTGATAGCTCTCGTGATCAATTCTGTATGGGAAATCCGTACGGCCCGGGAGGAACCGGATGGAATGGAGGAGGCT
GAGCCGCAGATCAATAGCGAAACTGCCAATGGCGGTTGGATCAAAGCTTGTATCCGCCTTTTGATTGGCATCCTGTTGTT
GACCGTCGGATCGAACCAGCTGGTTAAAGGGGCAAGCGATGCTGCTTCACTCTTAGGAGTGAGTGAGACCGTCATCGGAC
TCACGATCGTGTCTGCTGGCACCTCAATGCCGGAATTGTTCACATCTTTGGTGGCTGCTCTACGAGGCCGCACAGATCTA
GCGATTGGCAATGTGGTGGGCAGCAGCTTGCTCAATCAACTTCTGGTCCTGAGCAGTTGTGCCTTGATCTCTGGTGGGAC
TGGGCTGCAAGTCGATCGCTTGATCATTGAGCGAGATCTACCTGTGATGGTGCTCACCACGCTTGCCTGCATGCCAATTT
TTTGGACCAGGGGACGAATTAGCCGGCTTGAAGGAGGGATCCTCGTAGGGCTCTACGTTTTTTATGTGATCGATCAAGTT
CTCCCCCGCACCCTACCCACTTGGCAGGATGGATTTCGATTGACAATGCTCTGCATTGTTCTACCGATTGTTCTCGTTAT
GATTGTGCTTCAAGCCTTGTTGTATTGGCGTCAACTGCGCAGAGAACGAATAACTCATAGTGTTGATTGA

Upstream 100 bases:

>100_bases
CAAAACCTTAAGATCGTCTTACGCAAACAACCATGATTTTTGAGAGAGAGGGTCAGGGTTTGCGTTCAGATGCAGAATCC
CTTTAGTCCGTTCGGATCAC

Downstream 100 bases:

>100_bases
TCTAGCAAACATCCTGCTGAAGATGGAGACTAATCGAGTCAACTGATGCGAAGATACCGATATCATGTGACACGAACATC
AGAACATATACATCGCCAAG

Product: CaCA family sodium/calcium exchanger

Products: Na (I) [Cytoplasm]; Ca (II) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPQFLEPALQVLAGIGLLFGGGELFVQGSVALALIFGIPELVIGLTVVALGTCAPELFVSVSSVLKGSDALAVSNVVGSN
IFNVMVVLGSSALVLPLRVESRLVRRDVPLLLAVSAAVWGMASAGRVTWQAGVALLIALVINSVWEIRTAREEPDGMEEA
EPQINSETANGGWIKACIRLLIGILLLTVGSNQLVKGASDAASLLGVSETVIGLTIVSAGTSMPELFTSLVAALRGRTDL
AIGNVVGSSLLNQLLVLSSCALISGGTGLQVDRLIIERDLPVMVLTTLACMPIFWTRGRISRLEGGILVGLYVFYVIDQV
LPRTLPTWQDGFRLTMLCIVLPIVLVMIVLQALLYWRQLRRERITHSVD

Sequences:

>Translated_369_residues
MPQFLEPALQVLAGIGLLFGGGELFVQGSVALALIFGIPELVIGLTVVALGTCAPELFVSVSSVLKGSDALAVSNVVGSN
IFNVMVVLGSSALVLPLRVESRLVRRDVPLLLAVSAAVWGMASAGRVTWQAGVALLIALVINSVWEIRTAREEPDGMEEA
EPQINSETANGGWIKACIRLLIGILLLTVGSNQLVKGASDAASLLGVSETVIGLTIVSAGTSMPELFTSLVAALRGRTDL
AIGNVVGSSLLNQLLVLSSCALISGGTGLQVDRLIIERDLPVMVLTTLACMPIFWTRGRISRLEGGILVGLYVFYVIDQV
LPRTLPTWQDGFRLTMLCIVLPIVLVMIVLQALLYWRQLRRERITHSVD
>Mature_368_residues
PQFLEPALQVLAGIGLLFGGGELFVQGSVALALIFGIPELVIGLTVVALGTCAPELFVSVSSVLKGSDALAVSNVVGSNI
FNVMVVLGSSALVLPLRVESRLVRRDVPLLLAVSAAVWGMASAGRVTWQAGVALLIALVINSVWEIRTAREEPDGMEEAE
PQINSETANGGWIKACIRLLIGILLLTVGSNQLVKGASDAASLLGVSETVIGLTIVSAGTSMPELFTSLVAALRGRTDLA
IGNVVGSSLLNQLLVLSSCALISGGTGLQVDRLIIERDLPVMVLTTLACMPIFWTRGRISRLEGGILVGLYVFYVIDQVL
PRTLPTWQDGFRLTMLCIVLPIVLVMIVLQALLYWRQLRRERITHSVD

Specific function: Unknown

COG id: COG0530

COG function: function code P; Ca2+/Na+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1789587, Length=294, Percent_Identity=34.3537414965986, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004481
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 39242; Mature: 39111

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQFLEPALQVLAGIGLLFGGGELFVQGSVALALIFGIPELVIGLTVVALGTCAPELFVS
CCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSSVLKGSDALAVSNVVGSNIFNVMVVLGSSALVLPLRVESRLVRRDVPLLLAVSAAVWG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHC
MASAGRVTWQAGVALLIALVINSVWEIRTAREEPDGMEEAEPQINSETANGGWIKACIRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LIGILLLTVGSNQLVKGASDAASLLGVSETVIGLTIVSAGTSMPELFTSLVAALRGRTDL
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
AIGNVVGSSLLNQLLVLSSCALISGGTGLQVDRLIIERDLPVMVLTTLACMPIFWTRGRI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
SRLEGGILVGLYVFYVIDQVLPRTLPTWQDGFRLTMLCIVLPIVLVMIVLQALLYWRQLR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERITHSVD
HHHHCCCCC
>Mature Secondary Structure 
PQFLEPALQVLAGIGLLFGGGELFVQGSVALALIFGIPELVIGLTVVALGTCAPELFVS
CCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSSVLKGSDALAVSNVVGSNIFNVMVVLGSSALVLPLRVESRLVRRDVPLLLAVSAAVWG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHC
MASAGRVTWQAGVALLIALVINSVWEIRTAREEPDGMEEAEPQINSETANGGWIKACIRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LIGILLLTVGSNQLVKGASDAASLLGVSETVIGLTIVSAGTSMPELFTSLVAALRGRTDL
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
AIGNVVGSSLLNQLLVLSSCALISGGTGLQVDRLIIERDLPVMVLTTLACMPIFWTRGRI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
SRLEGGILVGLYVFYVIDQVLPRTLPTWQDGFRLTMLCIVLPIVLVMIVLQALLYWRQLR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERITHSVD
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; Ca (II) [Cytoplasm] [C]

Specific reaction: Na (I) [Periplasm] + Ca (II) [Cytoplasm] = Na (I) [Cytoplasm] + Ca (II) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]