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The map label for this gene is leuB [H]

Identifier: 124023434

GI number: 124023434

Start: 1516996

End: 1518069

Strand: Reverse

Name: leuB [H]

Synonym: P9303_17341

Alternate gene names: 124023434

Gene position: 1518069-1516996 (Counterclockwise)

Preceding gene: 124023435

Following gene: 124023433

Centisome position: 56.59

GC content: 55.68

Gene sequence:

>1074_bases
ATGCGCCAGCACCGCATCGTTCTGCTGCCTGGTGATGGTATCGGTCCTGAAATCACTGCTGTAGCAAAGTTGTTACTTGA
TGCAGCAGGCGAACAACACGGTTTCAAGCTGAATTTCGAGCAACACCCCATAGGCGGTGTTGCCATAGATGCAAGCGGAT
CGCCGTTGCCCGCCAGCACCCTTGAGGCATGTCAAGCCAGTGATGCGGTGCTCCTTGCAGCAATTGGTAGCCCTCGCTTT
GATGCTCTGCCCAGGGAGCAACGGCCGGAGACAGGCCTGCTATCCCTTCGTGCTGGACTGAAACTATTTGCGAACCTCAG
GCCGGTGACGATCCTGCCGGCCCTGATCGATGCCAGCAGCCTGAAAGCAAATGTGATCAAAGGGGTTGATCTAATGGTGG
TGCGGGAGCTCACAGGCGGGATCTATTTCGGCCAACCGAAAGGGCGGATTGAGGCCGATGGTGATGAGCGCGCTTTCAAC
ACAATGACCTACTCCAGAACTGAGGTGGACCGAATCGCCAAGGTCGCTTTCGACCTCGCCCGCGATCGCAGCGGAAAGCT
TTGTTCGATAGATAAGGCAAACGTGCTTGATGTCAGTCAACTGTGGCGTGATCGTGTCGATGCTTTGGCTGCCAACTATG
GAGATGTAGAGCTCAGCCACATGTACGTGGACAATGCCGCGATGCAATTGGTGCGCAACCCCAGGCAATTTGACGTGCTG
CTTACCGGCAACCTGTTCGGAGACATCCTTAGTGACGAAGCAGCCATGCTTACCGGCTCGATTGGCATGCTGCCCTCAGC
TTCGCTCGGCAGCGAAGGACCAGGACTGTTCGAACCGGTTCATGGTTCCGCACCCGATATTGCAGGACAAGACCTGGCCA
ATCCAATGGCAATGGTGCTTTGCGCAGCAATGATGCTGAGAATTGGCTTAAAGGAAAACGATGCCGCCACGGCGCTTGAA
GGATCAGTAGAGCGTGTTCTCGCGGCAGGTTTCCGTACAGGTGACCTCATGAGTGAGGGTTGTACCCAACTGGGCTGCGC
AGGGATGGGTGAGCAATTGCTGCAAGCGCTTTAA

Upstream 100 bases:

>100_bases
TTCAGCAAATTACCGGAGATGGCCAAAATGTTGAGGAAGCTGACAAGAGATACACCTCAGTAACCTCACAGGAGGCACAA
GCCATTTTGGAGTCCTCCAC

Downstream 100 bases:

>100_bases
AGGATTTAAAACCCTGATGCGCAACTGCTGATGTTGCCAAATTGATCTGTTTGCCGCATGACCTGCCAAAATCTCTGGGC
CTGTCCTGCCTGCGTCGATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDAAGEQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFDLARDRSGKLCSIDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAATALE
GSVERVLAAGFRTGDLMSEGCTQLGCAGMGEQLLQAL

Sequences:

>Translated_357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDAAGEQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFDLARDRSGKLCSIDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAATALE
GSVERVLAAGFRTGDLMSEGCTQLGCAGMGEQLLQAL
>Mature_357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDAAGEQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFDLARDRSGKLCSIDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAATALE
GSVERVLAAGFRTGDLMSEGCTQLGCAGMGEQLLQAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=31.7142857142857, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI28178821, Length=308, Percent_Identity=30.5194805194805, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=30.9677419354839, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758582, Length=324, Percent_Identity=28.0864197530864, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178838, Length=324, Percent_Identity=28.0864197530864, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=145, Percent_Identity=37.2413793103448, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=51.7045454545455, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=36.9318181818182, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787381, Length=409, Percent_Identity=25.1833740831296, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=351, Percent_Identity=33.048433048433, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=31.3609467455621, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=319, Percent_Identity=30.0940438871473, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=307, Percent_Identity=28.6644951140065, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=46.1971830985916, Blast_Score=279, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=34.7457627118644, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=31.8452380952381, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324709, Length=349, Percent_Identity=30.0859598853868, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=34.0236686390533, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=34.0236686390533, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24661184, Length=347, Percent_Identity=31.9884726224784, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=28.8629737609329, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=28.8629737609329, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=26.6081871345029, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=26.6081871345029, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=26.6081871345029, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=26.6081871345029, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=26.7062314540059, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37779; Mature: 37779

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHRIVLLPGDGIGPEITAVAKLLLDAAGEQHGFKLNFEQHPIGGVAIDASGSPLPAST
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCCCCCCHHH
LEACQASDAVLLAAIGSPRFDALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASS
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFNTMTYSRTEVDRIAKVAFDLA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHH
RDRSGKLCSIDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
HCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVL
EECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
CAAMMLRIGLKENDAATALEGSVERVLAAGFRTGDLMSEGCTQLGCAGMGEQLLQAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHC
>Mature Secondary Structure
MRQHRIVLLPGDGIGPEITAVAKLLLDAAGEQHGFKLNFEQHPIGGVAIDASGSPLPAST
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCCCCCCHHH
LEACQASDAVLLAAIGSPRFDALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASS
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFNTMTYSRTEVDRIAKVAFDLA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHH
RDRSGKLCSIDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
HCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVL
EECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
CAAMMLRIGLKENDAATALEGSVERVLAAGFRTGDLMSEGCTQLGCAGMGEQLLQAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]