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The map label for this gene is sir [H]

Identifier: 124023373

GI number: 124023373

Start: 1458756

End: 1460552

Strand: Reverse

Name: sir [H]

Synonym: P9303_16711

Alternate gene names: 124023373

Gene position: 1460552-1458756 (Counterclockwise)

Preceding gene: 124023375

Following gene: 124023359

Centisome position: 54.44

GC content: 53.26

Gene sequence:

>1797_bases
GTGACCGAAGGGCAGACAACATCTGGCCAAGCGGCAGATCTCAAGTTCGCAGCTTGTCTCGCCAGTGGAGCAGACCTGAG
CAAATTTGAACAATTCAAGGCTGACAGCTGCTACTTACTAGACCCCCTGGCTGCTGAACTTGAGAACAGCAGTGATCACT
TCAGCAACGACGCCGTTCAGCTGCTCAAATTCCACGGAAGCTATCAACAGGACAACCGAGATAACCGCCAGAAAGGTGAG
GGTAAGGATTGGCAAATGATGCTGCGTCTGCGCAGCCCTGGCGGACGTATCCCACCATCACTGTTTTTAGCCTTAGATGA
TCTGTCCAATCGCCTTGGCAATGGGAGCCTGCGGGCCACTACCCGCCAGGCGTTCCAGATTCATGGTGTCCGCAAGGAGG
ACCTGCGCGAAGTTATCAGCACAATCATCCGATCAATGGGATCAACCCTGGCGGCTTGTGGTGATATCACTCGCAACGTT
ATGGCACCAGCAGCTCCCTATGAGAAAGGTGGTTATCCAGCTGCTCGACAACTTGCCAATGAAATCGCCGATGTTCTCAG
CCCAACGGCAGCGGAGGGCTCCTATCTGGACCTATGGGTGAATGGAAGTGCGACCTACCGCATCCGCCCCTCCAGGCGAG
TGAAAAAAGTTCGTACCCGCCAGCATCAGGGCAACGTCTTCAGTGGAGATACCAAGGAACCTCTATATGGCAAGACCTAT
TTGCCGCGCAAATTTAAGTGCGCCGTCACCGTGCCAGGCGATAACTCAGTTGATCTACTTACCAATGACATCGGTCTAGT
TGTCTTTACTGCTGCAAACGGTGAAATCAAGGGTTGCAATGTCTATGTGGGTGGCGGCATGGGTCGAACCCACAACAATG
AACAGACATTCGCCCGTACAGCTGATCCTCTTGGCTATGTGGCGGCAGAGCATGTATTGGATTTGGTGCAGTCGATTTTG
GCCTTGCAACGCGACTACGGAGACCGAAAGACAAGGCGTCATGCCCGCATGAAATATCTTCTCAATGATCAGGGGATCAG
CTGGTTTAAACAGGAACTCAAGAGCAAATACTTCCTTCATCCAATCAAAGCGTTAAGGCCTGAACCAAAGAGCAAACTTG
AGGACTATCTGGGCTGGCATCAGCAAAGCGTTGGCCAATGGTTTGTTGGTTTGCCACTGCTCTCTGGACGCTTGGAAGGA
GATCTCAAGAAAGGACTCCGCCAACTGGTGGAGACCTTTCAACTTGAAATCCGCCTAACACCGAATCAGGATCTACTGCT
CTGCAATATCGGGACGTCACAACGGTCCAGTGTTCGCAAAGGTCTAGCCGCTCTAGGCATCCGCGCCCCAGAGGCTCCGG
CGATGCTGGCACGGCATGCAATTGCTTGCCCTGCGTTGCCCTTATGTGGTCTGGCAGTAACCGAAGCAGAGAGAACGCTG
CCTGTCTTGCTTGAGCGAATTGATGCTCAGCTGCGACGTCTTGAAATTACTAAATCGATCCTGATCCGCATGACAGGTTG
TCCCAATGGATGCGCGCGGCCCTACATGGCCGAGCTTGCTCTAGTGGGAAGTGGTGTCGATCAATATCAACTCTGGCTGG
GAGGCACCCCAAACCTGCAACGTCTTGCCAAACCCTATCTACAGCGGATGCCCCTCGATCAACTCGAGAGCACACTTGAG
CCCCTATTGCTGAGCTGGAAGCAAGCTGGTGGTCGACGCAGCCTCGGAGATCATGTCAACAAACTTGGCGATCAAGCCGT
CCTTGGTTTGCTGTCTGGTACTGGCAAGGCTCCATAG

Upstream 100 bases:

>100_bases
ACACGGAGGCGAGCAATCAAGATCGCCCAACTGTATTGAAGGGAGCAGCTCAACAGGTCGTGTGCATTTCGCTACGGTGT
ATTAGGAAAATTTTGACGCC

Downstream 100 bases:

>100_bases
ATTGCTTCAGCGAAACCCTTTCTCCACGCCCAGAGTTGATCTCCATAGCTGAAATGCCACCACTCATTCGGGTGCTGAGA
AAACCCAGCTTTACCCATGG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTEGQTTSGQAADLKFAACLASGADLSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGE
GKDWQMMLRLRSPGGRIPPSLFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNV
MAPAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGSATYRIRPSRRVKKVRTRQHQGNVFSGDTKEPLYGKTY
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSIL
ALQRDYGDRKTRRHARMKYLLNDQGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEG
DLKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAIACPALPLCGLAVTEAERTL
PVLLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLE
PLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGTGKAP

Sequences:

>Translated_598_residues
MTEGQTTSGQAADLKFAACLASGADLSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGE
GKDWQMMLRLRSPGGRIPPSLFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNV
MAPAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGSATYRIRPSRRVKKVRTRQHQGNVFSGDTKEPLYGKTY
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSIL
ALQRDYGDRKTRRHARMKYLLNDQGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEG
DLKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAIACPALPLCGLAVTEAERTL
PVLLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLE
PLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGTGKAP
>Mature_597_residues
TEGQTTSGQAADLKFAACLASGADLSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGEG
KDWQMMLRLRSPGGRIPPSLFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVM
APAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGSATYRIRPSRRVKKVRTRQHQGNVFSGDTKEPLYGKTYL
PRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSILA
LQRDYGDRKTRRHARMKYLLNDQGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGD
LKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAIACPALPLCGLAVTEAERTLP
VLLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLEP
LLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGTGKAP

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=583, Percent_Identity=32.2469982847341, Blast_Score=296, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6322597, Length=591, Percent_Identity=36.7174280879865, Blast_Score=332, Evalue=1e-91,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 65856; Mature: 65725

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGQTTSGQAADLKFAACLASGADLSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQ
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEECHHHHHHCCCCCCCCHHHHH
LLKFHGSYQQDNRDNRQKGEGKDWQMMLRLRSPGGRIPPSLFLALDDLSNRLGNGSLRAT
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVMAPAAPYEKGGYPAARQLAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EIADVLSPTAAEGSYLDLWVNGSATYRIRPSRRVKKVRTRQHQGNVFSGDTKEPLYGKTY
HHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFART
CCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHH
ADPLGYVAAEHVLDLVQSILALQRDYGDRKTRRHARMKYLLNDQGISWFKQELKSKYFLH
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
PIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGDLKKGLRQLVETFQLEIRLT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEC
PNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAIACPALPLCGLAVTEAERTL
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
PVLLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHH
RLAKPYLQRMPLDQLESTLEPLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGTGKAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEGQTTSGQAADLKFAACLASGADLSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQ
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEECHHHHHHCCCCCCCCHHHHH
LLKFHGSYQQDNRDNRQKGEGKDWQMMLRLRSPGGRIPPSLFLALDDLSNRLGNGSLRAT
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVMAPAAPYEKGGYPAARQLAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EIADVLSPTAAEGSYLDLWVNGSATYRIRPSRRVKKVRTRQHQGNVFSGDTKEPLYGKTY
HHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFART
CCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHH
ADPLGYVAAEHVLDLVQSILALQRDYGDRKTRRHARMKYLLNDQGISWFKQELKSKYFLH
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
PIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGDLKKGLRQLVETFQLEIRLT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEC
PNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAIACPALPLCGLAVTEAERTL
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
PVLLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHH
RLAKPYLQRMPLDQLESTLEPLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGTGKAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]