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The map label for this gene is ispE

Identifier: 124023320

GI number: 124023320

Start: 1418555

End: 1419514

Strand: Direct

Name: ispE

Synonym: P9303_16181

Alternate gene names: 124023320

Gene position: 1418555-1419514 (Clockwise)

Preceding gene: 124023319

Following gene: 124023321

Centisome position: 52.88

GC content: 51.56

Gene sequence:

>960_bases
ATGAGCATATCTAGCCCTTCTGCTGCTTCAGGGCATCTGGTTAGCGTCTCGGCGCCAGCCAAGATCAATCTCCATCTTGA
GGTGCTTGGCCTCAGGTCTGATGGTTTTCATGAGCTAGCGATGGTGATGCAAAGCATCGAACTCGCTGATCAACTTCATT
TCCGCAATACGGCTGATGGCACCATCAGCCTGCGCTGCGATGATTCCAGCCTCAGCACTGCTGGCGATAATTTGATCGTG
CAAGCTGCGCATTTATTACGTGAGCGCTCAGGGTTCTCTGAACTTGGTGCCGCGATTGAATTGCAAAAACGCATCCCAAT
TGGAGCTGGTCTTGCGGGCGGCTCAAGTGATGGTGCAGCAACACTGGTGGGGTTAAACGGTCTCTGGAATCTCAATTTTT
CTCAGGGTCAACTTGAAGGTTTTGCGGCTGAGCTTGGCTCCGATATGCCCTTTTGCCTGGCAGGTGGAAGCCAATTGTGT
TTCGGTCGTGGGGAAAGGTTGGAATCGCTACAAGCGATGCAAGCATCAATGGCCGTGGTGTTGGTGAAGGATCCATCAGT
GAGCGTTTCAACCCCTTGGGCTTATGGACGCTGTAAGGAACTTTTCAGGAGTCGTTATCTTTCACAGGAATCTGATTTTG
AGCAACGTCGTCAGCAGCTCAGAGAATCTTCTTGGCTGAATCCTTTGCGGGCTGATGATCCACCACCTCTGCACAACGAT
CTTCAGGCTGTGGTTGCACCCGAAGTATTTGCTGTGCAAACCACATTGAAGTTGCTCAGTGATTTGCCTGGTTCTCTTGC
TGTAGCGATGAGTGGATCTGGTCCAAGCTGTTTTGCCCTTTTTGCTGACGTTGATTCAGCTCAGGCAGCCCTTAAGCGCC
AACAGCCTGCCTTCGACGCAGCTGGTTTAAGCAGTTGGTGCTGCGCGTTCCGCTCTGAAGGCATCAAACTGGAAGCATGA

Upstream 100 bases:

>100_bases
TTGGCATCAGCCTTCAGCAACGCCCCCAAGAACTGTCCCCTGCCACTTGGGTGGAATTGGCCAGGGGTTTGAATCGGGCG
GATCTAGTAGACCCTGAGCC

Downstream 100 bases:

>100_bases
GCGATTCCAAAAACAGCTCTTTTGAGACGGCAGAAGCAGAGTCATCCACCACATCATTGGCAGAGACGCCTGCGACACCA
CGCAAGGGCCCGCTGAGTTT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSISSPSAASGHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGTISLRCDDSSLSTAGDNLIV
QAAHLLRERSGFSELGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLC
FGRGERLESLQAMQASMAVVLVKDPSVSVSTPWAYGRCKELFRSRYLSQESDFEQRRQQLRESSWLNPLRADDPPPLHND
LQAVVAPEVFAVQTTLKLLSDLPGSLAVAMSGSGPSCFALFADVDSAQAALKRQQPAFDAAGLSSWCCAFRSEGIKLEA

Sequences:

>Translated_319_residues
MSISSPSAASGHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGTISLRCDDSSLSTAGDNLIV
QAAHLLRERSGFSELGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLC
FGRGERLESLQAMQASMAVVLVKDPSVSVSTPWAYGRCKELFRSRYLSQESDFEQRRQQLRESSWLNPLRADDPPPLHND
LQAVVAPEVFAVQTTLKLLSDLPGSLAVAMSGSGPSCFALFADVDSAQAALKRQQPAFDAAGLSSWCCAFRSEGIKLEA
>Mature_318_residues
SISSPSAASGHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADGTISLRCDDSSLSTAGDNLIVQ
AAHLLRERSGFSELGAAIELQKRIPIGAGLAGGSSDGAATLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCF
GRGERLESLQAMQASMAVVLVKDPSVSVSTPWAYGRCKELFRSRYLSQESDFEQRRQQLRESSWLNPLRADDPPPLHNDL
QAVVAPEVFAVQTTLKLLSDLPGSLAVAMSGSGPSCFALFADVDSAQAALKRQQPAFDAAGLSSWCCAFRSEGIKLEA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=180, Percent_Identity=36.6666666666667, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PROM3 (A2CA52)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017627.1
- ProteinModelPortal:   A2CA52
- SMR:   A2CA52
- STRING:   A2CA52
- GeneID:   4778340
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_16181
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK02534
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33882; Mature: 33750

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 21-21 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISSPSAASGHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADG
CCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TISLRCDDSSLSTAGDNLIVQAAHLLRERSGFSELGAAIELQKRIPIGAGLAGGSSDGAA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCFGRGERLESLQAMQASMAVV
EEEECCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHCEEEE
LVKDPSVSVSTPWAYGRCKELFRSRYLSQESDFEQRRQQLRESSWLNPLRADDPPPLHND
EEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LQAVVAPEVFAVQTTLKLLSDLPGSLAVAMSGSGPSCFALFADVDSAQAALKRQQPAFDA
HHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCC
AGLSSWCCAFRSEGIKLEA
CCHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
SISSPSAASGHLVSVSAPAKINLHLEVLGLRSDGFHELAMVMQSIELADQLHFRNTADG
CCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TISLRCDDSSLSTAGDNLIVQAAHLLRERSGFSELGAAIELQKRIPIGAGLAGGSSDGAA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLVGLNGLWNLNFSQGQLEGFAAELGSDMPFCLAGGSQLCFGRGERLESLQAMQASMAVV
EEEECCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHCEEEE
LVKDPSVSVSTPWAYGRCKELFRSRYLSQESDFEQRRQQLRESSWLNPLRADDPPPLHND
EEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LQAVVAPEVFAVQTTLKLLSDLPGSLAVAMSGSGPSCFALFADVDSAQAALKRQQPAFDA
HHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCC
AGLSSWCCAFRSEGIKLEA
CCHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA