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The map label for this gene is rlmH

Identifier: 124023305

GI number: 124023305

Start: 1406439

End: 1406891

Strand: Reverse

Name: rlmH

Synonym: P9303_16031

Alternate gene names: 124023305

Gene position: 1406891-1406439 (Counterclockwise)

Preceding gene: 124023308

Following gene: 124023304

Centisome position: 52.44

GC content: 52.32

Gene sequence:

>453_bases
TTGCTGCAGTTGTTCTTGAGAAACTGTGCCTTTAGAATTATCGCCGTTGGCAAGGTACGCAAAGGCTGGATTCAAGACGG
CCTAGCGATGTATCAAAAGCGCCTACCAGGGCTCATGATCACAGAGGTTCGTGATGCATCTCTGCCGCGAGAAGCAGAAG
CGATCCTGGCCGCACTGAATAGCAACGAAGTGCTGGTCCCTCTCAGCGAGGAAGGGGAAGCTCTGACTTCAGTGTCCTTT
GCTAAACGCCTTGAAAAATATGGCTCTCAACGTCTTGCCTTTGTGATTGGTGGCGCTGATGGCCTATCAGCAGAGCTGAA
AAACTCAACCCAATGGCAGCTCAGCTTGTCTGCAATGACGCTTCCCCATGAACTTGCTCGCCTGTTGCTTGTGGAGCAGC
TCTACCGAGCCCAGACCATCCTGCAAGGAGGTAAATACCACAGGGGCTCATGA

Upstream 100 bases:

>100_bases
TGCTTCGCTCTGCGGTATTCCGCCTGGAGGACTTCAAGCAAAATTCTCTTCTGGGGATCTGTTGAACAGTGCATCTAAGC
GAGCATCATGGAGATGGATC

Downstream 100 bases:

>100_bases
TTCAGAAGATCTGAATTGCGATCACTGATAAAACAGCTGCATTTGATTAATGCGATAACGCTTGAGCACACTGAAAAGGA
AATCAGGTTAAGGCAATGAT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MLQLFLRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLMITEVRDASLPREAEAILAALNSNEVLVPLSEEGEALTSVSF
AKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTILQGGKYHRGS

Sequences:

>Translated_150_residues
MLQLFLRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLMITEVRDASLPREAEAILAALNSNEVLVPLSEEGEALTSVSF
AKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTILQGGKYHRGS
>Mature_150_residues
MLQLFLRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLMITEVRDASLPREAEAILAALNSNEVLVPLSEEGEALTSVSF
AKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTILQGGKYHRGS

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PROM3 (A2CA37)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017612.1
- ProteinModelPortal:   A2CA37
- STRING:   A2CA37
- GeneID:   4776457
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_16031
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   CLSK919387
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16613; Mature: 16613

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLFLRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLMITEVRDASLPREAEAILAALN
CHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCC
SNEVLVPLSEEGEALTSVSFAKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMT
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCEEEEEEHHHH
LPHELARLLLVEQLYRAQTILQGGKYHRGS
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLQLFLRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLMITEVRDASLPREAEAILAALN
CHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCC
SNEVLVPLSEEGEALTSVSFAKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMT
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCEEEEEEHHHH
LPHELARLLLVEQLYRAQTILQGGKYHRGS
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA