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The map label for this gene is ruvA [H]

Identifier: 124023288

GI number: 124023288

Start: 1390956

End: 1391657

Strand: Reverse

Name: ruvA [H]

Synonym: P9303_15861

Alternate gene names: 124023288

Gene position: 1391657-1390956 (Counterclockwise)

Preceding gene: 124023290

Following gene: 124023287

Centisome position: 51.88

GC content: 54.84

Gene sequence:

>702_bases
ATGGATGACATCAAGAGCAACCTCATGATCGGCTGGCTGCAAGGGCAAAAAGTAGAAGCCTGGCAGCAAGGGACTCGGCA
AGGAGTTGTTTTGGCATGTGCTGGTGTTGGTTACGAGGTTCAGATTGCACCGCGCCATCTCAGCGAAATGGAGCACGGGC
AAAACACATTCATCCTCTGGATCCATCAGGTGCAGCGCGATGATGGATCCAGCTTGTTTGGCTTCCCGGAACGACGAGAA
CGGGACATGTTTCGTACCTTGATTGGTGTTAGCGGAGTGGGGCCCCAGATGGCCCTCGCTCTTCTAGAGGAATGTCAGAC
CGGAGAACTTGTAGAGGCGATTGTGCAGGGAGACCTACGCAAGCTTTGTCAGGCTCAAGGTGTAGGCAAGCGCACAGCCG
AACGTCTTGCGGTGGAATTGCGCACAAAGTTGGCTGAATTTAGTTGTCGTGATCCTGGAATGTCCCTGGTGGATAACGGT
GTGATCGATTCGCACCAGCTCAAGGATTCAAGTCTGCATGAGCTACAGATCACCCTCGGGGGCCTTGGTTACGAAGATCT
TGAAATACGCCGTGCCATAAGGGCTGTGGCCAGCGGTGCTGCAATAGGAGCCAGGGACATGCCTGAGTCTGTTCCCGCCA
TCGACGACACTGACGCCTGGCTGAGAGCAAGCCTGCGCTGGCTTAGTCAGGAAGCTGCGTAA

Upstream 100 bases:

>100_bases
AGGAATTGAGTTTTAGACCGGTCACGCAAAGCAAAACAAGCTCCAGAAGCTCTGAGCAGGACCTCGAACGATCAAGCAGA
GGCTGGACAGATCAGGCAGC

Downstream 100 bases:

>100_bases
ACAATGGTCCTCAAAGAGTTAGATTGTTTTTTTGGTCTCTTAGGGCAAAACGCCAAGCATGTCGCTCGATACAACAGAAA
AGCAACAGCTGATCAACGCC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDDIKSNLMIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRE
RDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNG
VIDSHQLKDSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGARDMPESVPAIDDTDAWLRASLRWLSQEAA

Sequences:

>Translated_233_residues
MDDIKSNLMIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRE
RDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNG
VIDSHQLKDSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGARDMPESVPAIDDTDAWLRASLRWLSQEAA
>Mature_233_residues
MDDIKSNLMIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRE
RDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNG
VIDSHQLKDSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGARDMPESVPAIDDTDAWLRASLRWLSQEAA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family [H]

Homologues:

Organism=Escherichia coli, GI1788168, Length=191, Percent_Identity=31.413612565445, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994 [H]

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 25666; Mature: 25666

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDIKSNLMIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILW
CCCCCCCEEEEEECCCEEHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCEEEEE
IHQVQRDDGSSLFGFPERRERDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLKDSSLHELQITLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCCCCEEEEEEEC
GLGYEDLEIRRAIRAVASGAAIGARDMPESVPAIDDTDAWLRASLRWLSQEAA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDIKSNLMIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILW
CCCCCCCEEEEEECCCEEHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCEEEEE
IHQVQRDDGSSLFGFPERRERDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLKDSSLHELQITLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCCCCEEEEEEEC
GLGYEDLEIRRAIRAVASGAAIGARDMPESVPAIDDTDAWLRASLRWLSQEAA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]