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The map label for this gene is gatA

Identifier: 124023284

GI number: 124023284

Start: 1385050

End: 1386510

Strand: Direct

Name: gatA

Synonym: P9303_15821

Alternate gene names: 124023284

Gene position: 1385050-1386510 (Clockwise)

Preceding gene: 124023283

Following gene: 124023285

Centisome position: 51.63

GC content: 54.89

Gene sequence:

>1461_bases
ATGGCGATCGCTGAATGGCGTCAGCAACTAAAGCTCGGCGAGGTTTCCGCCAGGGAACTCATAGATCACCAACTTGCTCG
TATCGCAGTGGTGGATCCCACTCTGCACGCTTTTCTGGATGTCACAGCAGAGCGAGCTCGTGCCGATGCCGATCGCATCG
ATGAAGCCTTGGCTGCGGGTGAGTCTCTACCACCTCTGGCTGGTGTGCCTTTGGCAATTAAGGACAACCTCTGCACGAAA
GGAATTCGCACCACCTGTTCCAGTCGAATGCTGGAAACCTTTGTGCCCCCCTATGAATCCACCGTCACTGAGCGCCTCTG
GCAGGCCGGTGCTGTGCTTCTCGGGAAAACAAATCTCGATGAGTTTGCCATGGGTAGCTCGACCGAGACATCTGCGTTTG
GGGCTACATCCAATCCCTGGGATATCAGCCGTGTGCCGGGTGGCAGTTCTGGTGGCAGTGCAGCAGCTGTTGCGGCAGGT
GAATGCATGGCTGCCTTGGGTTCGGATACTGGGGGCTCAATCCGTCAGCCCGCTTCATTTTGTGGGGTCGTAGGCCTTAA
ACCCACCTATGGCCGTGTCAGTCGTTGGGGTTTGGTGGCTTTTGCCAGTTCTCTTGATCAAGTTGGACCGTTCACAACCA
ACGTGGCTGATGCTGCTGAGCTTTTGCAAGTGATCGCAGGATCTGATCCTCGTGACTCAACTTGTCTCAATGTTGCTGTT
CCCGATTATTGCTCTGCCCTTTCGCAACCAATGTCTGGTGTGCGCATCGGCTTGATTCGAGAGTGTTTTGATCAGAACGG
CTTGGATGCGCAAGTGAAGAGCACAGTTTTGGAAGCTGCAGAGAAGCTGCAATCCTTGGGAGCGGAACTGGTTGAGGTGA
GTTGCCCACGCTTTAGCGATGGCATCGCCACCTATTACGTGATTGCCCCGTCAGAGGCTTCAGCCAACTTGGCGCGTTAC
GACGGCGTGAAGTACGGCTACCGAGCTGAAGGAGCTGATGCTCTAGCCGCGATGACAGCCCGAAGTCGCGCAGAGGGTTT
TGGTAGCGAGGTGCAGCGACGCATCCTGATCGGCACCTATGCCCTGTCGGCTGGTTACATGGATGCTTACTACAAGAAAG
CCCAGCAGGTCCGCACATTGATCCGTCAAGATTTTGATGCTGCCTTCCAAACGGTCGATGTGCTGCTGACACCGACATCA
CCTACCACTGCATTCCAAGTCGGTGCCCACGCTGATGATCCACTTGCCATGTACCTGGCTGACCTGCTGACTATCCCAGC
CAATCTTGCTGGCTTACCTGCCATCAGTCTTCCCTGTGGATTCGATGACGATGGCCTTCCCATCGGGGTGCAGTTGATTG
CCAATGTGCTGGAGGAGTCGCGCCTTCTACAAGTGGCTTTTCACTATGAGCAATCAGCAAACGTGATGACAAATCACCCG
CAGGGAAACTTCATTCCCTAA

Upstream 100 bases:

>100_bases
CCTCGTGCGGTGTCGTCGCGTTGAGCCGCTGACGATCGCAGTCGAAGGCTGGTCAGACGACTGATAGCGTCGCTGCTGCA
CAGCACAGCACCGCAAGCCG

Downstream 100 bases:

>100_bases
ACGATCTCTTGCACTAGATGCAGCGTTTGGCTACATGAACAACCCTGCATCTTGCGCCTCCTCTTAGGCTGGGGGCATGG
CATTCGTTCCTCTTCACAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAIAEWRQQLKLGEVSARELIDHQLARIAVVDPTLHAFLDVTAERARADADRIDEALAAGESLPPLAGVPLAIKDNLCTK
GIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAG
ECMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKSTVLEAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARY
DGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTS
PTTAFQVGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQSANVMTNHP
QGNFIP

Sequences:

>Translated_486_residues
MAIAEWRQQLKLGEVSARELIDHQLARIAVVDPTLHAFLDVTAERARADADRIDEALAAGESLPPLAGVPLAIKDNLCTK
GIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAG
ECMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKSTVLEAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARY
DGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTS
PTTAFQVGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQSANVMTNHP
QGNFIP
>Mature_485_residues
AIAEWRQQLKLGEVSARELIDHQLARIAVVDPTLHAFLDVTAERARADADRIDEALAAGESLPPLAGVPLAIKDNLCTKG
IRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLDEFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGE
CMAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAVP
DYCSALSQPMSGVRIGLIRECFDQNGLDAQVKSTVLEAAEKLQSLGAELVEVSCPRFSDGIATYYVIAPSEASANLARYD
GVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTYALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSP
TTAFQVGAHADDPLAMYLADLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQSANVMTNHPQ
GNFIP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=487, Percent_Identity=40.041067761807, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=26.2948207171315, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=232, Percent_Identity=33.1896551724138, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=39.3181818181818, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=24.7967479674797, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=235, Percent_Identity=33.1914893617021, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=316, Percent_Identity=30.379746835443, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=315, Percent_Identity=26.984126984127, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=312, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=287, Percent_Identity=29.9651567944251, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=232, Percent_Identity=25.4310344827586, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=35.1674641148325, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=457, Percent_Identity=32.6039387308534, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=190, Percent_Identity=34.7368421052632, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648113, Length=473, Percent_Identity=43.9746300211416, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24644968, Length=492, Percent_Identity=27.6422764227642, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=25.3549695740365, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=25.3549695740365, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=25.3549695740365, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=25.3549695740365, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=25.7606490872211, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=25.7606490872211, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=25.7606490872211, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=26.3888888888889, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648441, Length=435, Percent_Identity=26.4367816091954, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648439, Length=435, Percent_Identity=26.4367816091954, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=23.9263803680982, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PROM3 (A2CA16)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017591.1
- ProteinModelPortal:   A2CA16
- SMR:   A2CA16
- STRING:   A2CA16
- GeneID:   4776097
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_15821
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51440; Mature: 51308

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 74-74 ACT_SITE 149-149 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAEWRQQLKLGEVSARELIDHQLARIAVVDPTLHAFLDVTAERARADADRIDEALAAG
CCHHHHHHHHHHCCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ESLPPLAGVPLAIKDNLCTKGIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCH
EFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGECMAALGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKSTVLEAAEKLQSLGAELVEVSCPRFSD
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSPTTAFQVGAHADDPLAMYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQSANVMTNHP
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
QGNFIP
CCCCCC
>Mature Secondary Structure 
AIAEWRQQLKLGEVSARELIDHQLARIAVVDPTLHAFLDVTAERARADADRIDEALAAG
CHHHHHHHHHHCCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ESLPPLAGVPLAIKDNLCTKGIRTTCSSRMLETFVPPYESTVTERLWQAGAVLLGKTNLD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCH
EFAMGSSTETSAFGATSNPWDISRVPGGSSGGSAAAVAAGECMAALGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQVGPFTTNVADAAELLQVIAGSDPRDSTCLNVAV
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
PDYCSALSQPMSGVRIGLIRECFDQNGLDAQVKSTVLEAAEKLQSLGAELVEVSCPRFSD
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRAEGADALAAMTARSRAEGFGSEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALSAGYMDAYYKKAQQVRTLIRQDFDAAFQTVDVLLTPTSPTTAFQVGAHADDPLAMYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDDDGLPIGVQLIANVLEESRLLQVAFHYEQSANVMTNHP
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
QGNFIP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA