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The map label for this gene is carA [H]

Identifier: 124023279

GI number: 124023279

Start: 1381074

End: 1382213

Strand: Direct

Name: carA [H]

Synonym: P9303_15771

Alternate gene names: 124023279

Gene position: 1381074-1382213 (Clockwise)

Preceding gene: 124023278

Following gene: 124023280

Centisome position: 51.48

GC content: 53.86

Gene sequence:

>1140_bases
ATGATTGCTTCATCGCGAAATTCGGCGCTCTTGGTGCTTGCTGATGGCACGGTGCTTGAGGGGGAGGCCTTTGGTCATCG
CGGTGCCGCGATGGGCGAGGTGGTTTTCAACACCGGCATGACGGGTTACCAGGAAGTACTTACAGATCCCAGTTACTCCG
GTCAGTTGGTCACATTCACCTATCCAGAGTTGGGCAACACAGGCGTAAATCTTGATGATCAAGAATCATCCCGGCCTCAT
GCAAGGGGTGTCATCGCCCGTCAACTCTCCCCAACGCCGAGCAGTTGGCGTTGCCAACAGTCGCTGCAGACTTGGCTTGA
GGAGCAAAGAGTTGTTGGTATCTCAGGTGTTGATACCAGGGCGCTTGTGAGACATCTGCGCGAGGTCGGAGCCATGAATG
GCGTCATAAGCAGCGATGGTTCCACCCCTAAGCAGTTGATGGAACTGTTGCAGCAGGCTCCATCGATGGAGGGATTAAAC
CTTGCAGATCAGGTTTCAACGAAAGATCCATACAGCTGGAGATCGACTTGTGCTGTGGGCTTTGATCAAAGGTTGAAGCG
ACACATTGATTCGCCCTATCGCGTTGTCGCGATTGATTTCGGTATCAAGCGGGCCATACTCGACCGTTTGGTCGCTCACG
GTTGTGAGGTCACCGTCCTGCCGGCCAGTTCCGATTTAGCCACCGTGTTGAGTTCACAACCCGAGGGCGTATTTCTATCT
AATGGACCTGGTGACCCTGCTGCCGTGACTGGTGGTATTGCCCTGGTTAAAGATCTTCTCAATAAGGTTGATATCCCCCT
GTTTGGTATTTGCCTAGGTCATCAGATTATTGGGCTTGCCATGGGTGGCCGAACTTTCAAATTGGCCTATGGCCATCGCG
GGCTCAACCATCCCTGCGGCACAACGGGGCAGGTGGAGATCACCAGTCAAAACCATGGATTCGCACTTGACGCCTCGTCT
CTGTCACAGGTCAGCGTGGTTGTGACCCATCTCAATCTCAATGACCGCACTGTTGCCGCGATAGCGCATCGGGATCGACC
TGTTTTTGGTGTGCAATACCACCCGGAGGCGAGTCCTGGTCCTCATGATGCAGACCACCACTTCGCTCACTTTGTTGAGC
TGATGGCAGATCGTCGCTAA

Upstream 100 bases:

>100_bases
GCAGGAGGGCCTCCCCTGGCAGCGGCTAGAAGGGCTCCGCATGGCACTTGATCATCAAATTGGAGAATGAGACTGCGATG
ACTTCGGGATCCTTCTGCTG

Downstream 100 bases:

>100_bases
AGCGTGGTGCGGATCACTCAGTATCACTACACTTCGTGCCATTGCGTCCCGGGGGACTGGTCCCATCACAGAATTGCAGC
GACTGACCGTTTCTCTAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIASSRNSALLVLADGTVLEGEAFGHRGAAMGEVVFNTGMTGYQEVLTDPSYSGQLVTFTYPELGNTGVNLDDQESSRPH
ARGVIARQLSPTPSSWRCQQSLQTWLEEQRVVGISGVDTRALVRHLREVGAMNGVISSDGSTPKQLMELLQQAPSMEGLN
LADQVSTKDPYSWRSTCAVGFDQRLKRHIDSPYRVVAIDFGIKRAILDRLVAHGCEVTVLPASSDLATVLSSQPEGVFLS
NGPGDPAAVTGGIALVKDLLNKVDIPLFGICLGHQIIGLAMGGRTFKLAYGHRGLNHPCGTTGQVEITSQNHGFALDASS
LSQVSVVVTHLNLNDRTVAAIAHRDRPVFGVQYHPEASPGPHDADHHFAHFVELMADRR

Sequences:

>Translated_379_residues
MIASSRNSALLVLADGTVLEGEAFGHRGAAMGEVVFNTGMTGYQEVLTDPSYSGQLVTFTYPELGNTGVNLDDQESSRPH
ARGVIARQLSPTPSSWRCQQSLQTWLEEQRVVGISGVDTRALVRHLREVGAMNGVISSDGSTPKQLMELLQQAPSMEGLN
LADQVSTKDPYSWRSTCAVGFDQRLKRHIDSPYRVVAIDFGIKRAILDRLVAHGCEVTVLPASSDLATVLSSQPEGVFLS
NGPGDPAAVTGGIALVKDLLNKVDIPLFGICLGHQIIGLAMGGRTFKLAYGHRGLNHPCGTTGQVEITSQNHGFALDASS
LSQVSVVVTHLNLNDRTVAAIAHRDRPVFGVQYHPEASPGPHDADHHFAHFVELMADRR
>Mature_379_residues
MIASSRNSALLVLADGTVLEGEAFGHRGAAMGEVVFNTGMTGYQEVLTDPSYSGQLVTFTYPELGNTGVNLDDQESSRPH
ARGVIARQLSPTPSSWRCQQSLQTWLEEQRVVGISGVDTRALVRHLREVGAMNGVISSDGSTPKQLMELLQQAPSMEGLN
LADQVSTKDPYSWRSTCAVGFDQRLKRHIDSPYRVVAIDFGIKRAILDRLVAHGCEVTVLPASSDLATVLSSQPEGVFLS
NGPGDPAAVTGGIALVKDLLNKVDIPLFGICLGHQIIGLAMGGRTFKLAYGHRGLNHPCGTTGQVEITSQNHGFALDASS
LSQVSVVVTHLNLNDRTVAAIAHRDRPVFGVQYHPEASPGPHDADHHFAHFVELMADRR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=38.8451443569554, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI169790915, Length=388, Percent_Identity=35.8247422680412, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI21361331, Length=388, Percent_Identity=35.8247422680412, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=47.8835978835979, Blast_Score=345, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI193204318, Length=382, Percent_Identity=38.4816753926702, Blast_Score=234, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=387, Percent_Identity=37.4677002583979, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=35.4114713216958, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=37.0179948586118, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=37.0179948586118, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40758; Mature: 40758

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASSRNSALLVLADGTVLEGEAFGHRGAAMGEVVFNTGMTGYQEVLTDPSYSGQLVTFT
CCCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEE
YPELGNTGVNLDDQESSRPHARGVIARQLSPTPSSWRCQQSLQTWLEEQRVVGISGVDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCHH
ALVRHLREVGAMNGVISSDGSTPKQLMELLQQAPSMEGLNLADQVSTKDPYSWRSTCAVG
HHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHCC
FDQRLKRHIDSPYRVVAIDFGIKRAILDRLVAHGCEVTVLPASSDLATVLSSQPEGVFLS
HHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEE
NGPGDPAAVTGGIALVKDLLNKVDIPLFGICLGHQIIGLAMGGRTFKLAYGHRGLNHPCG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCC
TTGQVEITSQNHGFALDASSLSQVSVVVTHLNLNDRTVAAIAHRDRPVFGVQYHPEASPG
CCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCC
PHDADHHFAHFVELMADRR
CCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIASSRNSALLVLADGTVLEGEAFGHRGAAMGEVVFNTGMTGYQEVLTDPSYSGQLVTFT
CCCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEE
YPELGNTGVNLDDQESSRPHARGVIARQLSPTPSSWRCQQSLQTWLEEQRVVGISGVDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCHH
ALVRHLREVGAMNGVISSDGSTPKQLMELLQQAPSMEGLNLADQVSTKDPYSWRSTCAVG
HHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHCC
FDQRLKRHIDSPYRVVAIDFGIKRAILDRLVAHGCEVTVLPASSDLATVLSSQPEGVFLS
HHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEE
NGPGDPAAVTGGIALVKDLLNKVDIPLFGICLGHQIIGLAMGGRTFKLAYGHRGLNHPCG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCC
TTGQVEITSQNHGFALDASSLSQVSVVVTHLNLNDRTVAAIAHRDRPVFGVQYHPEASPG
CCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCC
PHDADHHFAHFVELMADRR
CCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]