Click here to switch to the map view.

The map label for this gene is vacB [H]

Identifier: 124023151

GI number: 124023151

Start: 1244201

End: 1246591

Strand: Reverse

Name: vacB [H]

Synonym: P9303_14471

Alternate gene names: 124023151

Gene position: 1246591-1244201 (Counterclockwise)

Preceding gene: 124023152

Following gene: 124023150

Centisome position: 46.47

GC content: 48.89

Gene sequence:

>2391_bases
ATGAAGTTCACGGTCGCTGACCTGCTTGACCAGTTACCCCCCACTGGGGGACTGGAGATCAAAAAACTCGAAAAAATTCT
CAAGCTCACCACAAAGGCCGATCGCGACGGTCTTGAAACAGCTCTTCAGGGTCTCTTGAGACTCGGCATTGTCAACAACG
AAGAAGCCGGTGCAATAAAGCGCAGTGATGACGAGTCACTGATTGAAGCTCGTTTGCGCTGCAGCAGTAAAGGATTTTGT
TTTGCCCTAAGAGATGATGGGGGAGACGATATCTATATTCGCGATCATCAGCTCAACCATGCCTGGAACGGAGATCGGGT
ACTAGTCCGCATCACAAGAGATGGGGGACGTCGAAGATCGCCAGAAGGTGGGGTGCAATGCATCCTCGAAAGGACCACCA
CCAGCTTGCTTGGTCATGTTGAACGCAAAGATCAAAATGTCCTTGCAATACCTCTTGATGACCGCATCCTTGCAACGATC
CAACTACCAGATGGTGATAAAGCCCATCTGAATGATGGGGAGCAGACAAGCGTGGTGGAGGTGAAGCTTGACCGCTATCC
CATTGCCCAGTTCCCCGCTGAAGGGCATGTAGCGCGGTCACTTCCCTTGAATGGAGGCCCCTCAAGTGATCGTGATCTTC
TTCTTACCAAAGCCAACCTTCAAGATCGACCTGCTCCACCACGCAGCAGCCTAAAAACCCCAACGGCTAAACATCGGCAA
GATCTGAGCTCACAACCAGCGTTGCTCCTGCGCAGCTGGCAGGTATCTGATGCTCCACCCCTACCAGCGGTCTATGTGGA
GCCCCATGCAGGTGGCACACGTCTGTGGATTCATGCGCCTGCTGTAGCAGAACGTCTGAGTATTGGGAACAACCTGGATC
TCTGGCTAAGAGATCGAGCTGAAGCTCTCTGCCTTGGCGAAGTTTGGCATCCCCTACTCGGGCAAATTCTTTCAAAGGCC
TGTTCATTTAAAGTTGGGGAAATAAACGATGCAGTAAGCGTTGCTCTCGACATCTCAGCAGATGGTGAGGTGATTGATTG
GCATTTCAGTCTTAGCTCAATCAAGCCAGTAGCCGAAATCGGACCGAAGACCCTCACAGCTCTTAGCAACAGGAAACCAA
AGGCAAGAACAGTCCCTGCTGCTCTGAAGTCCGTCAAGGATCACTTAGCCCAACTCGAGACATTGATCTTTTGTGCTCGT
ACATTGCAAATGGGAGAACAGGCCAGTGGCTCTATAGAGCTGGATCTGGCTGTTCCAGAACTTCAATGTCTTGGCGACCT
CCGCTGGGCAGACCCTGATTCATATCGTCATCAATGGACGCTTCCTCTTGATCAAACTGACCCCCAATCAGTTTTGAGCC
CAATAATTAGAGCTGCTCATCGCGCCTGGGCACAACATGCCCAGAACTTGCAACTCCCTGGCCTGGTGATTGAGGCTTCA
GAAGCAGAAAACAGCACCTTGAACGACGTTGCTAAATCAGCATTAGCTCTTGATATTGCCTTGGAACTTGATGAAGAAGG
TAGTCCTGCTGCCACAGAGCTTGCAAAAGCATTTGCGACAACGGCCTGTCGAAGAGTGCTTGATCAGCAACTACGTCATG
CACTGCCAGAACCTGTGTTACGGCTCGCAATTCATGATAGTCAAAAAGAGAATGGAAACATTGAATCCGACCATGAAGAC
ACAAAGTCAATGGATACGAACCTACAATCACCTTGGTGCTGTCCAACTATCCATTACACCGATCTTGTTAATCAAGAGAT
CATAGTGTCACTTTTAAGTAACGGTAAAGATCGTCCAAATGTCCGACAAAAAGAAAAATTGGTTCTGGGGTCCCGTGAAT
GCTGGCAACAACTCAAATGGCCACTATTCAGCACAAGCCAAGCAAAGAACCTAAAAGAAATCTGTAGTGAAAGCCTCGTG
CATCGCCTCAACACACTGCGCCGTCAGGCTGAAGAGCTTCGACAAGACCTGATCGCCATGGTTCAGGCACGTATCGTGGA
ACCCCTTGTTGGGGAAGAGCATCAGGGGGTAATAAGCGGGGTGCAGAGTTATGGGTTCTTTGTGGAGATTCCACCTTCTA
TGGCAGAGGGTCTTGTCCACGTCAGCTCTTTAAATGACGATTGGTACGAATATCGCTCACGTCAAAACCGATTGGTGGGT
CGGAAGAATCGCAAGATCTATCAACTTGGTGATCAAGTAAACGTGAAGGTTCTCAAAGTTGACGCCTTAAGGAACCAAAT
TGACCTCGAAGTTAATGGATCAGCAATGACTGTTGAAGCCAATATGAATGTTGATTCAGATCTAACCAATCAGGTAACAA
CAACAAAAAGTGAGGTCAAAGGCAAAATCACCAAGAATAATGAACAACTAGTCAGCTCAAGTGAGGCATAG

Upstream 100 bases:

>100_bases
AATTAGCTCACAGCCAATGTTGACTCTGCGCTGTCTTCGGATTCCTCTTTGGTGATGGCACCGCCGGTCATGAAGACTGG
TCTTCTACCTTCCTATTTCA

Downstream 100 bases:

>100_bases
TTTGTGGATCCCTATGTCCTTGCCGTTTCAGGAGCTTCAGCTCAACCTCTAGCTGAAAGATCGCTGCAGCTCTTGCTTGA
AAACAACCGTGACGTCCATT

Product: acetazolamide conferring resistance protein Zam

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MKFTVADLLDQLPPTGGLEIKKLEKILKLTTKADRDGLETALQGLLRLGIVNNEEAGAIKRSDDESLIEARLRCSSKGFC
FALRDDGGDDIYIRDHQLNHAWNGDRVLVRITRDGGRRRSPEGGVQCILERTTTSLLGHVERKDQNVLAIPLDDRILATI
QLPDGDKAHLNDGEQTSVVEVKLDRYPIAQFPAEGHVARSLPLNGGPSSDRDLLLTKANLQDRPAPPRSSLKTPTAKHRQ
DLSSQPALLLRSWQVSDAPPLPAVYVEPHAGGTRLWIHAPAVAERLSIGNNLDLWLRDRAEALCLGEVWHPLLGQILSKA
CSFKVGEINDAVSVALDISADGEVIDWHFSLSSIKPVAEIGPKTLTALSNRKPKARTVPAALKSVKDHLAQLETLIFCAR
TLQMGEQASGSIELDLAVPELQCLGDLRWADPDSYRHQWTLPLDQTDPQSVLSPIIRAAHRAWAQHAQNLQLPGLVIEAS
EAENSTLNDVAKSALALDIALELDEEGSPAATELAKAFATTACRRVLDQQLRHALPEPVLRLAIHDSQKENGNIESDHED
TKSMDTNLQSPWCCPTIHYTDLVNQEIIVSLLSNGKDRPNVRQKEKLVLGSRECWQQLKWPLFSTSQAKNLKEICSESLV
HRLNTLRRQAEELRQDLIAMVQARIVEPLVGEEHQGVISGVQSYGFFVEIPPSMAEGLVHVSSLNDDWYEYRSRQNRLVG
RKNRKIYQLGDQVNVKVLKVDALRNQIDLEVNGSAMTVEANMNVDSDLTNQVTTTKSEVKGKITKNNEQLVSSSEA

Sequences:

>Translated_796_residues
MKFTVADLLDQLPPTGGLEIKKLEKILKLTTKADRDGLETALQGLLRLGIVNNEEAGAIKRSDDESLIEARLRCSSKGFC
FALRDDGGDDIYIRDHQLNHAWNGDRVLVRITRDGGRRRSPEGGVQCILERTTTSLLGHVERKDQNVLAIPLDDRILATI
QLPDGDKAHLNDGEQTSVVEVKLDRYPIAQFPAEGHVARSLPLNGGPSSDRDLLLTKANLQDRPAPPRSSLKTPTAKHRQ
DLSSQPALLLRSWQVSDAPPLPAVYVEPHAGGTRLWIHAPAVAERLSIGNNLDLWLRDRAEALCLGEVWHPLLGQILSKA
CSFKVGEINDAVSVALDISADGEVIDWHFSLSSIKPVAEIGPKTLTALSNRKPKARTVPAALKSVKDHLAQLETLIFCAR
TLQMGEQASGSIELDLAVPELQCLGDLRWADPDSYRHQWTLPLDQTDPQSVLSPIIRAAHRAWAQHAQNLQLPGLVIEAS
EAENSTLNDVAKSALALDIALELDEEGSPAATELAKAFATTACRRVLDQQLRHALPEPVLRLAIHDSQKENGNIESDHED
TKSMDTNLQSPWCCPTIHYTDLVNQEIIVSLLSNGKDRPNVRQKEKLVLGSRECWQQLKWPLFSTSQAKNLKEICSESLV
HRLNTLRRQAEELRQDLIAMVQARIVEPLVGEEHQGVISGVQSYGFFVEIPPSMAEGLVHVSSLNDDWYEYRSRQNRLVG
RKNRKIYQLGDQVNVKVLKVDALRNQIDLEVNGSAMTVEANMNVDSDLTNQVTTTKSEVKGKITKNNEQLVSSSEA
>Mature_796_residues
MKFTVADLLDQLPPTGGLEIKKLEKILKLTTKADRDGLETALQGLLRLGIVNNEEAGAIKRSDDESLIEARLRCSSKGFC
FALRDDGGDDIYIRDHQLNHAWNGDRVLVRITRDGGRRRSPEGGVQCILERTTTSLLGHVERKDQNVLAIPLDDRILATI
QLPDGDKAHLNDGEQTSVVEVKLDRYPIAQFPAEGHVARSLPLNGGPSSDRDLLLTKANLQDRPAPPRSSLKTPTAKHRQ
DLSSQPALLLRSWQVSDAPPLPAVYVEPHAGGTRLWIHAPAVAERLSIGNNLDLWLRDRAEALCLGEVWHPLLGQILSKA
CSFKVGEINDAVSVALDISADGEVIDWHFSLSSIKPVAEIGPKTLTALSNRKPKARTVPAALKSVKDHLAQLETLIFCAR
TLQMGEQASGSIELDLAVPELQCLGDLRWADPDSYRHQWTLPLDQTDPQSVLSPIIRAAHRAWAQHAQNLQLPGLVIEAS
EAENSTLNDVAKSALALDIALELDEEGSPAATELAKAFATTACRRVLDQQLRHALPEPVLRLAIHDSQKENGNIESDHED
TKSMDTNLQSPWCCPTIHYTDLVNQEIIVSLLSNGKDRPNVRQKEKLVLGSRECWQQLKWPLFSTSQAKNLKEICSESLV
HRLNTLRRQAEELRQDLIAMVQARIVEPLVGEEHQGVISGVQSYGFFVEIPPSMAEGLVHVSSLNDDWYEYRSRQNRLVG
RKNRKIYQLGDQVNVKVLKVDALRNQIDLEVNGSAMTVEANMNVDSDLTNQVTTTKSEVKGKITKNNEQLVSSSEA

Specific function: Not known; control resistance to the carbonic anhydrase inhibitor acetazolamide [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082383, Length=766, Percent_Identity=22.5848563968668, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 88103; Mature: 88103

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTVADLLDQLPPTGGLEIKKLEKILKLTTKADRDGLETALQGLLRLGIVNNEEAGAIK
CCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
RSDDESLIEARLRCSSKGFCFALRDDGGDDIYIRDHQLNHAWNGDRVLVRITRDGGRRRS
CCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCC
PEGGVQCILERTTTSLLGHVERKDQNVLAIPLDDRILATIQLPDGDKAHLNDGEQTSVVE
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEE
VKLDRYPIAQFPAEGHVARSLPLNGGPSSDRDLLLTKANLQDRPAPPRSSLKTPTAKHRQ
EEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
DLSSQPALLLRSWQVSDAPPLPAVYVEPHAGGTRLWIHAPAVAERLSIGNNLDLWLRDRA
CCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECHHHHHHHHCCCCCCEEECCCC
EALCLGEVWHPLLGQILSKACSFKVGEINDAVSVALDISADGEVIDWHFSLSSIKPVAEI
HHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEEEEEEECCCCCEEEEEEEHHHCCHHHHC
GPKTLTALSNRKPKARTVPAALKSVKDHLAQLETLIFCARTLQMGEQASGSIELDLAVPE
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
LQCLGDLRWADPDSYRHQWTLPLDQTDPQSVLSPIIRAAHRAWAQHAQNLQLPGLVIEAS
HHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
EAENSTLNDVAKSALALDIALELDEEGSPAATELAKAFATTACRRVLDQQLRHALPEPVL
CCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLAIHDSQKENGNIESDHEDTKSMDTNLQSPWCCPTIHYTDLVNQEIIVSLLSNGKDRPN
HHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCC
VRQKEKLVLGSRECWQQLKWPLFSTSQAKNLKEICSESLVHRLNTLRRQAEELRQDLIAM
CHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQARIVEPLVGEEHQGVISGVQSYGFFVEIPPSMAEGLVHVSSLNDDWYEYRSRQNRLVG
HHHHHHHHHCCCCCHHHHHHHHHCCEEEEECHHHHHHEEEEECCCHHHHHHHHHHHCCCC
RKNRKIYQLGDQVNVKVLKVDALRNQIDLEVNGSAMTVEANMNVDSDLTNQVTTTKSEVK
CCCCEEEECCCCCCEEEEEEEHHCCEEEEEECCCEEEEEECCCCCCCHHHHHHCCHHHHC
GKITKNNEQLVSSSEA
CCEECCCHHHHCCCCC
>Mature Secondary Structure
MKFTVADLLDQLPPTGGLEIKKLEKILKLTTKADRDGLETALQGLLRLGIVNNEEAGAIK
CCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
RSDDESLIEARLRCSSKGFCFALRDDGGDDIYIRDHQLNHAWNGDRVLVRITRDGGRRRS
CCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCC
PEGGVQCILERTTTSLLGHVERKDQNVLAIPLDDRILATIQLPDGDKAHLNDGEQTSVVE
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEE
VKLDRYPIAQFPAEGHVARSLPLNGGPSSDRDLLLTKANLQDRPAPPRSSLKTPTAKHRQ
EEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
DLSSQPALLLRSWQVSDAPPLPAVYVEPHAGGTRLWIHAPAVAERLSIGNNLDLWLRDRA
CCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECHHHHHHHHCCCCCCEEECCCC
EALCLGEVWHPLLGQILSKACSFKVGEINDAVSVALDISADGEVIDWHFSLSSIKPVAEI
HHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEEEEEEECCCCCEEEEEEEHHHCCHHHHC
GPKTLTALSNRKPKARTVPAALKSVKDHLAQLETLIFCARTLQMGEQASGSIELDLAVPE
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
LQCLGDLRWADPDSYRHQWTLPLDQTDPQSVLSPIIRAAHRAWAQHAQNLQLPGLVIEAS
HHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
EAENSTLNDVAKSALALDIALELDEEGSPAATELAKAFATTACRRVLDQQLRHALPEPVL
CCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLAIHDSQKENGNIESDHEDTKSMDTNLQSPWCCPTIHYTDLVNQEIIVSLLSNGKDRPN
HHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCC
VRQKEKLVLGSRECWQQLKWPLFSTSQAKNLKEICSESLVHRLNTLRRQAEELRQDLIAM
CHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQARIVEPLVGEEHQGVISGVQSYGFFVEIPPSMAEGLVHVSSLNDDWYEYRSRQNRLVG
HHHHHHHHHCCCCCHHHHHHHHHCCEEEEECHHHHHHEEEEECCCHHHHHHHHHHHCCCC
RKNRKIYQLGDQVNVKVLKVDALRNQIDLEVNGSAMTVEANMNVDSDLTNQVTTTKSEVK
CCCCEEEECCCCCCEEEEEEEHHCCEEEEEECCCEEEEEECCCCCCCHHHHHHCCHHHHC
GKITKNNEQLVSSSEA
CCEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727754; 8905231 [H]