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The map label for this gene is prfC [H]

Identifier: 124023123

GI number: 124023123

Start: 1215535

End: 1217250

Strand: Direct

Name: prfC [H]

Synonym: P9303_14191

Alternate gene names: 124023123

Gene position: 1215535-1217250 (Clockwise)

Preceding gene: 124023122

Following gene: 124023125

Centisome position: 45.31

GC content: 49.01

Gene sequence:

>1716_bases
ATGCACTTGCACCCGCCAGATCTTCGTCACCGCTTGACCTCTCTTCCCGATTCCACGAGCCCGCCGATTGATTCGGCCTC
TTCAATCGATATAGATCAAGTCCTTGAGGCCTCGTTGGCTGAGGCCATTAGTCGACGCCGCAATTTTGCGATCATTTCCC
ATCCGGATGCGGGGAAGACCACCCTTACAGAAAAATTGTTGCTTTACGGAGGTGCGATCCAGCAAGCAGGGGCTGTTAAA
GCCCGTGGTGAGCAGCGCAAGGTGACCTCTGATTGGATGGAGTTGGAGAAGCAGCGGGGTATATCGATCACCTCCACCGT
GCTGCAGTTTGATTATGCAGATACCACAATTAACCTTCTTGATACCCCCGGACACCAGGATTTCTCCGAAGACACTTATC
GGACCCTGGCGGCTGCAGATAATGCGGTGATGTTGGAGGATGCAGCTAAGGGTTTAGAACCTCAGACAAGAAAATTGTTT
GAGGTCTGTCGCATGAGAAATATCCCAATCTTTACTTTCATAAACAAGATGGATCGTGCAGGGCGGGATCCTCTGTTGCT
GTTGGATGAGATTGAAGCAGAGTTGGAACTAATACCTTGGGCCGTGAATTGGCCTATTGGTAGTGGGGAGTTGTTTCGTG
GTGTTATTGATCGTCGAACTAGAAATGTCGTTCTTTTTACACGAGCGGAAAGGGGCCGGCAAGCCAGTGAACGACACCTT
CATCTTGACGATCCGGAATTACCAAGCCTTGTCGAGCAGGAGCTTTTGGACCAAGCTTTGGAAGAGCTAGAGCTCTTGGA
GGCAGCAGGTGCAGAGCTTGATCTGGAATTAGTGCATGCAGGCGAGTTGACACCAGTGTTTTTTGGTTCAGCAATGACCA
ATTTTGGGGTAAGGCCTTTCCTTGATGCCTTTCTTGAGATGGCGCAGCGTCCGGTAGCTCGTTCTACAAGTGAGGGCCCT
GTTGATCCTTTGCGATCGTCCTTTAGTGGCTTTGTGTTCAAATTACAGGCCAATATGGATCCTCGTCATCGTGATCGTGT
CGCCTTTGTAAGGGTCTGCAGCGGACGTTTTGAAAAGGACATGACGGTGCGCTTAGCACGAACCGGTAAATCCATACGTC
TATCTCGCCCACAGAAGTTATTTGGCCAAGAACGTTCGGTCGTTGATGATGCCTATCCAGGTGATGTTATCGGTCTAAAT
AATCCTGGTATGTTTGCGATTGGTGATACTCTGTATACAGGCTCTAGAGTTGAATATGAGGGTATACCATGCTTTAGTCC
TGAGATATTTAGCTGGTTACGTAACCCGAATCCTTCAGCATTTAAAAATTTTCGTAAAGGAGTCAATGAGTTAAGAGAGG
AAGGTGCTGTACAAATTCTCTACGACACTGATCAGAGCAAGCGGGATCCGATTCTTGCTGCAGTAGGTCAGTTGCAGCTT
GATGTTGTTCAACATCGTTTAGAAAACGAATATGGCGTTCAAACCCGCCTTGAACCTATGGGATTCCAGGTGGCACGGTG
GGTCAGTGATGGTTGGCCTGCCCTAGATGAGATGGGTCGAATATTTAATTGCAAGACCGTTCAGGATGCCTGGCAGCGCC
CTGTTCTCTTGTTCAAGAACGAGTGGAACCTTAACCAGCTTCTTGATGATCACCCTGCGTTGGAACTTAGTGCTGTTGCG
CCTGTGGTTAGTGGTGTAGAGCCGATCAGCCTCTGA

Upstream 100 bases:

>100_bases
AGAGGCTGAAATATCTGCCTTGCACCTGCCAAGAAGTGAATTCCTTGTCTTATGAATCTTAGACGCAGTCAAAGGCTTGA
TCATTTATTGCACCTTTACT

Downstream 100 bases:

>100_bases
GCGTTGGGCTTAATTCTGCTTCCCTGACGAAAAGAACTCGCGATTCACTGCTCTAGTTAGCCAGAATCGTTAAAGAATGT
TTTCAGTGGTAGTGGACAAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MHLHPPDLRHRLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADTTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRSSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL

Sequences:

>Translated_571_residues
MHLHPPDLRHRLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADTTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRSSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL
>Mature_571_residues
MHLHPPDLRHRLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADTTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRSSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=30.8176100628931, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI19923640, Length=569, Percent_Identity=26.8892794376098, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI25306287, Length=505, Percent_Identity=27.3267326732673, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI25306283, Length=432, Percent_Identity=29.8611111111111, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=30.9677419354839, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=35.036496350365, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=166, Percent_Identity=31.9277108433735, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790835, Length=470, Percent_Identity=50.8510638297872, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1789738, Length=499, Percent_Identity=28.8577154308617, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=34.0909090909091, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=32.7868852459016, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1790412, Length=137, Percent_Identity=31.3868613138686, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789737, Length=137, Percent_Identity=31.3868613138686, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=490, Percent_Identity=27.1428571428571, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=483, Percent_Identity=25.4658385093168, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=34, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=32.0987654320988, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=31.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=31.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=29.1925465838509, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=512, Percent_Identity=26.171875, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323320, Length=172, Percent_Identity=31.9767441860465, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=32.5, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=32.5, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582462, Length=488, Percent_Identity=30.7377049180328, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI221458488, Length=524, Percent_Identity=26.3358778625954, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=35, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585711, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574573, Length=159, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 64096; Mature: 64096

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00732 RIBOSOMAL_S16 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLHPPDLRHRLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKT
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELEKQRGISITSTVLQFDYADTTINLL
HHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHEEECCCCEEEEE
DTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRNIPIFTFINKMDRA
ECCCCCCCCHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
CCCCEEEHHHHCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHH
LDAFLEMAQRPVARSTSEGPVDPLRSSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKD
HHHHHHHHCCCHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
MTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLNNPGMFAIGDTLYTGSRVEYE
CEEEEECCCCCEECCCCHHHHCCCHHHHCCCCCCCEEECCCCCCEEECCEEECCCEEEEC
GIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKN
HHHHHHHCCCCCCCEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCEEEEEC
EWNLNQLLDDHPALELSAVAPVVSGVEPISL
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHLHPPDLRHRLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKT
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELEKQRGISITSTVLQFDYADTTINLL
HHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHEEECCCCEEEEE
DTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRNIPIFTFINKMDRA
ECCCCCCCCHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
CCCCEEEHHHHCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHH
LDAFLEMAQRPVARSTSEGPVDPLRSSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKD
HHHHHHHHCCCHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
MTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLNNPGMFAIGDTLYTGSRVEYE
CEEEEECCCCCEECCCCHHHHCCCHHHHCCCCCCCEEECCCCCCEEECCEEECCCEEEEC
GIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKN
HHHHHHHCCCCCCCEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCEEEEEC
EWNLNQLLDDHPALELSAVAPVVSGVEPISL
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA