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The map label for this gene is sphR [H]

Identifier: 124022658

GI number: 124022658

Start: 859484

End: 860188

Strand: Reverse

Name: sphR [H]

Synonym: P9303_09491

Alternate gene names: 124022658

Gene position: 860188-859484 (Counterclockwise)

Preceding gene: 124022660

Following gene: 124022657

Centisome position: 32.06

GC content: 56.03

Gene sequence:

>705_bases
ATGCAGACCACGCCAAAGCAGCCCTCACGGGTCCTTGTGGTGGAACCTCACCAAACTCTTCGCACGGTGCTGGTCCATCG
CCTCCGTCAGGACGGCCATCTCACCGCTGCAGTGGCATCCGCCGTGGAAGCCCTTGACGTCTGTCGAGATCAGTCACCGG
ATCTTCTCGTGAGTGCTGAGTTACTTGAGAGGAGTTCTGCAATGCGACTCTCTCATCAGCTCCGTTGTCCTGTGATCGTG
CTAACAGCTCGAACAGGCACAGAGCCCATGGTGGGCCTCCTGAATGAAGGCGCCGACGATGTGTTGCGCAAACCATTTGG
ACTTGAAGAACTCGCCGCACGCTGTCGCACCTTGCTGAAACGGGGACATAGCGGTTTGCAGGAGCGCGTCACAGTGGGCC
CCCTTGAAGTCCACTTGTTGCTCAGACAAGTGACTCTGCGCGATAAACCTGTGGAATTAAGCCCCAGAGAATTTGCACTT
CTCTGTGCACTTTTAATGCCTCCAGGTATCGTACGAAGCCGCCAGGAATTATTGCGAATGGCTTGGCCTCCCTTCAGCGG
CGGGCCTCGTTCTGTAGACACCCAGATCCTTACCTTGCGACGCAAACTTGAGCAAGCTGGGCTTGGCGAAGGAGGTGGCA
TCACCACCATGCGCCAGCAGGGCTATCGATTCAGCCTGGATACTCTTCCTGATTCTGAATCATAA

Upstream 100 bases:

>100_bases
GGTTTAGCGTAAGCGCGATCTGTGATCACATGTGACCTCCATTCCCCGCAATCTTCATTCCACTGAGGACGCCCAAGTGT
CTTCATCGGGGTCTTTGCAG

Downstream 100 bases:

>100_bases
ATATTGGAGATCAAAACTATCGATTAGCCAATGATTAAAGACCCTGGTCGAAGTGACCAAAACAGATTCTAAGCATTACT
ACAAGTTCATGATTTATTAT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVSAELLERSSAMRLSHQLRCPVIV
LTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPREFAL
LCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMRQQGYRFSLDTLPDSES

Sequences:

>Translated_234_residues
MQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVSAELLERSSAMRLSHQLRCPVIV
LTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPREFAL
LCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMRQQGYRFSLDTLPDSES
>Mature_234_residues
MQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVSAELLERSSAMRLSHQLRCPVIV
LTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLKRGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPREFAL
LCALLMPPGIVRSRQELLRMAWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMRQQGYRFSLDTLPDSES

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=30.0448430493274, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=30.2222222222222, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786911, Length=231, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789809, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1790552, Length=148, Percent_Identity=35.8108108108108, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=29.5454545454545, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367329, Length=169, Percent_Identity=37.2781065088757, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=28.6919831223629, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789402, Length=131, Percent_Identity=37.4045801526718, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25884; Mature: 25884

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVSAE
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHH
LLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLK
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
RGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPREFALLCALLMPPGIVRSRQELLRM
HCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
AWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMRQQGYRFSLDTLPDSES
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MQTTPKQPSRVLVVEPHQTLRTVLVHRLRQDGHLTAAVASAVEALDVCRDQSPDLLVSAE
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHH
LLERSSAMRLSHQLRCPVIVLTARTGTEPMVGLLNEGADDVLRKPFGLEELAARCRTLLK
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
RGHSGLQERVTVGPLEVHLLLRQVTLRDKPVELSPREFALLCALLMPPGIVRSRQELLRM
HCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
AWPPFSGGPRSVDTQILTLRRKLEQAGLGEGGGITTMRQQGYRFSLDTLPDSES
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]