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The map label for this gene is leuA

Identifier: 124022627

GI number: 124022627

Start: 835096

End: 836718

Strand: Direct

Name: leuA

Synonym: P9303_09181

Alternate gene names: 124022627

Gene position: 835096-836718 (Clockwise)

Preceding gene: 124022625

Following gene: 124022628

Centisome position: 31.13

GC content: 53.67

Gene sequence:

>1623_bases
ATGGCCAAGGATCCAGGCCGCGTTTTGATTTTTGACACCACCCTCAGGGATGGGGAGCAGTCCCCTGGTGCAAGTCTCAA
TCTCGAGGAAAAGCTGGCTATTGCCCAGCAACTCGCTCGTTTGGGTGTGGATGTGATTGAAGCGGGCTTTCCTTTTGCCA
GCCAGGGTGATTTTTCTGCCGTGCAACGTATTGCCCAGCAGGTTGGCGGGGATGAAGGCCCAATCATTTGTGGCTTGGCT
AGGGCTTCGCGTGCTGATATCAAGGCTTGTGCTGATGCCGTTGCCCCAGCCCCTCGACGACGAATTCACACATTCATTGC
CACGAGTGATATTCACTTGGAGCACAAGCTGCGTAAATCTCGGGCGGAGGTTCTGGCGATCGTTCCAGAGATGGTCGCCT
ACGCCCGCTCACTCGTTGATGATGTTGAGTTCTCCTGTGAGGATGCCGCTCGAAGCGACCCTGAGTTTCTTTATGAGGTG
ATCGAGGCTGCGATTGCTGCGGGGGCGGGCACCATCAATATCCCCGACACAGTTGGTTTTACAACTCCCTCGGAGTTCGG
GGCGTTGATTGCTGGTATCGATTGCCATGTTCCAAATATGAATGAGGCCATTCTTTCTGTTCATGGTCACAACGATCTCG
GACTGGCTGTAGCCAATTTTTTAGAAGCTGTGAAAAGTGGAGCCCGGCAGTTTGAATGCACGATCAACGGCATTGGAGAG
CGTGCAGGGAATGCTGCGCTTGAGGAGCTGGTGATGGCTTTGTATGTGAGGCGGCGTTATTTCAATCCCTTCTTTGGGCG
TGAATCTGATAGCCCTACTCCTCTAACGGCCGTTCGCACGGAGGAAATCACCAAGACATCTCGATTGGTCTCAAATCTCA
CCGGCATGGTGGTGCAGCCCAATAAGGCGATTGTCGGCAGCAATGCTTTCGCCCATGAGTCAGGTATTCATCAAGATGGT
GTGCTTAAAAATCGTCTTACTTATGAAATTGTTGATGCCCGTACGGTTGGGCTGACCGACAATCGGATCTCGCTGGGCAA
GCTAAGTGGTCGCAGTGCCGTCCGAGCTCGCCTTGAGGAGCTCGGTTACGACTTGACTCGGGAGGATCTTGACGAAGCCT
TTGCACGATTTAAGGATCTGGCCGATCGCAAGCGCGATATAACCGATCGAGATCTTGAGGCCATTGTCAGCGAACAGGTC
CAGCAGCCTGAAGCTCGTTTTCAGTTACGACTGGTGCAAGTCAGTTGCGGTAGCAGTCTTAGGCCTACGGCTACTGTCAT
TCTTGCGCAAGAGGATGGACAGGAGCAGACTGCCGCTGCAGTGGGCACCGGTCCAGTAGATGCTGTCTGTCGTGCATTGA
ATGCTCTGGCTGGCGAACCTAATGAGCTGATCGAGTTCTCGGTTAAGTCGGTAACGGAAGGGATTGATGCGATGGGTGAA
GTCACGATTCGGCTACGTCGGGATGGGCAGCTTTTCTCAGGTCATTCCGCTGACACTGATGTGGTGGTTGCTGCAGCTCA
AGCATTTGTGAATGCGCTTAATCGGCTGGTGGCAGGTTGCGGTCGCCAGTCTCTTCATCCCCAACATGATGCTGTGCTAG
CTGATCGACGGCCAGGCATCTGA

Upstream 100 bases:

>100_bases
CGGACCTTGGCTTGCTGAGTTGTCGCTTCGCAGTAGGAAGTCCCCCCTTAGACTCGCTTAACTCATAGTCATTTCGACTA
ACTCCCTTAAGGGTGCTGTC

Downstream 100 bases:

>100_bases
ACAATGGGTCGTTTTCCCTATGTAGAGATGAGGCGTAAGAGCTTGGCTTCGCCACTGCTGAAAGGTCTTCAGTTAGGGGT
CCTTATCGTGTTGGCGCTTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSAVQRIAQQVGGDEGPIICGLA
RASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEV
IEAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
RAGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDG
VLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQV
QQPEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI

Sequences:

>Translated_540_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSAVQRIAQQVGGDEGPIICGLA
RASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEV
IEAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
RAGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDG
VLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQV
QQPEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI
>Mature_539_residues
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSAVQRIAQQVGGDEGPIICGLAR
ASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVI
EAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGER
AGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDGV
LKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQ
QPEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGEV
TIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=48.7329434697856, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320019, Length=404, Percent_Identity=27.7227722772277, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=410, Percent_Identity=28.2926829268293, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=501, Percent_Identity=26.5469061876248, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=500, Percent_Identity=25, Blast_Score=111, Evalue=3e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PROM3 (A2C859)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016934.1
- ProteinModelPortal:   A2C859
- SMR:   A2C859
- STRING:   A2C859
- GeneID:   4778733
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_09181
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 58068; Mature: 57937

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSA
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAQQVGGDEGPIICGLARASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVIEAAIAAGAGTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCC
TTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
CCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGSNAFAHESGIHQDGVLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEE
CCEEECCCCHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQQPEARFQLRLVQVSCGSSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCC
RPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
CCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFSA
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAQQVGGDEGPIICGLARASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVIEAAIAAGAGTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCC
TTPSEFGALIAGIDCHVPNMNEAILSVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
CCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELVMALYVRRRYFNPFFGRESDSPTPLTAVRTEEITKTSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGSNAFAHESGIHQDGVLKNRLTYEIVDARTVGLTDNRISLGKLSGRSAVRARLEE
CCEEECCCCHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQQPEARFQLRLVQVSCGSSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCC
RPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
CCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADRRPGI
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA